Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient
In this study, strain B2D isolated from a dental plaque sample of a human patient was studied for its general characteristics, taxonomic identification, genome features, and resistome profile. The bacterium exhibited antibiotic resistance to all beta-lactam antibiotics, nitrofuran, and sulfonamides...
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| Format: | Article |
| Language: | English |
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HH Publisher
2024-04-01
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| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/941 |
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| author | Share Yuan Goh Kah-Ooi Chua Saad Ahmed Khan Noor Hayaty Abu Kasim Yvonne Jing Mei Liew Yin Yin Lau Kar-Wai Hong Wai-Fong Yin Hoi-Sen Yong Chien-Yi Chang Kok-Gan Chan |
| author_facet | Share Yuan Goh Kah-Ooi Chua Saad Ahmed Khan Noor Hayaty Abu Kasim Yvonne Jing Mei Liew Yin Yin Lau Kar-Wai Hong Wai-Fong Yin Hoi-Sen Yong Chien-Yi Chang Kok-Gan Chan |
| author_sort | Share Yuan Goh |
| collection | DOAJ |
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In this study, strain B2D isolated from a dental plaque sample of a human patient was studied for its general characteristics, taxonomic identification, genome features, and resistome profile. The bacterium exhibited antibiotic resistance to all beta-lactam antibiotics, nitrofuran, and sulfonamides, with high minimum inhibitory concentrations. It was only sensitive to the fluoroquinolone ciprofloxacin and intermediately susceptible to aminoglycoside tobramycin. A preliminary identification through 16S rRNA gene sequences revealed that it shared the highest sequence identity with Elizabethkingia anophelis subsp. endophytica JM-87T (100%) and Elizabethkingia anophelis subsp. anophelis R26T (99.31%). The draft genome of strain B2D was approximately 3.9 Mbp with 50 contigs and 35.5% GC content. A 16S rRNA gene and core genes-based phylogenetic analyses revealed a close phylogenetic relationship between strain B2D and the other Elizabethkingia type strains. An above species level threshold average nucleotide identity value confirmed its taxonomic identity as Elizabethkingia anophelis. Furthermore, we conducted a resistome analysis of strain B2D and Elizabethkingia type strains, revealing the presence of widespread antibiotic resistance genes, including beta-lactamases and genes associated with cationic antiseptic resistance and glycopeptide resistance. Overall, the multidrug resistant profile of strain B2D as elucidated and confirmed through whole genome analysis indicated its potential as a reservoir of beta-lactamase genes. Moreover, its presence within dental plaque in the human oral cavity prompts speculation regarding its role as an opportunistic pathogen capable of causing infections, particularly in immunocompromised individuals.
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| format | Article |
| id | doaj-art-c9437d9890bc47e0b8f44e62029f3c51 |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-c9437d9890bc47e0b8f44e62029f3c512025-02-04T08:39:40ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492024-04-017110.36877/pmmb.a0000440Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of PatientShare Yuan GohKah-Ooi ChuaSaad Ahmed KhanNoor Hayaty Abu KasimYvonne Jing Mei LiewYin Yin LauKar-Wai HongWai-Fong YinHoi-Sen YongChien-Yi ChangKok-Gan Chan In this study, strain B2D isolated from a dental plaque sample of a human patient was studied for its general characteristics, taxonomic identification, genome features, and resistome profile. The bacterium exhibited antibiotic resistance to all beta-lactam antibiotics, nitrofuran, and sulfonamides, with high minimum inhibitory concentrations. It was only sensitive to the fluoroquinolone ciprofloxacin and intermediately susceptible to aminoglycoside tobramycin. A preliminary identification through 16S rRNA gene sequences revealed that it shared the highest sequence identity with Elizabethkingia anophelis subsp. endophytica JM-87T (100%) and Elizabethkingia anophelis subsp. anophelis R26T (99.31%). The draft genome of strain B2D was approximately 3.9 Mbp with 50 contigs and 35.5% GC content. A 16S rRNA gene and core genes-based phylogenetic analyses revealed a close phylogenetic relationship between strain B2D and the other Elizabethkingia type strains. An above species level threshold average nucleotide identity value confirmed its taxonomic identity as Elizabethkingia anophelis. Furthermore, we conducted a resistome analysis of strain B2D and Elizabethkingia type strains, revealing the presence of widespread antibiotic resistance genes, including beta-lactamases and genes associated with cationic antiseptic resistance and glycopeptide resistance. Overall, the multidrug resistant profile of strain B2D as elucidated and confirmed through whole genome analysis indicated its potential as a reservoir of beta-lactamase genes. Moreover, its presence within dental plaque in the human oral cavity prompts speculation regarding its role as an opportunistic pathogen capable of causing infections, particularly in immunocompromised individuals. https://journals.hh-publisher.com/index.php/pmmb/article/view/941 |
| spellingShingle | Share Yuan Goh Kah-Ooi Chua Saad Ahmed Khan Noor Hayaty Abu Kasim Yvonne Jing Mei Liew Yin Yin Lau Kar-Wai Hong Wai-Fong Yin Hoi-Sen Yong Chien-Yi Chang Kok-Gan Chan Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient Progress in Microbes and Molecular Biology |
| title | Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient |
| title_full | Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient |
| title_fullStr | Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient |
| title_full_unstemmed | Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient |
| title_short | Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient |
| title_sort | genomic and resistome analyses of elizabethkingia anophelis strain b2d isolated from dental plaque of patient |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/941 |
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