Establishing an Immune System Conferring DNA and RNA Virus Resistance in Plants Using CRISPR/Cas12a Multiplex Gene Editing

Establishing an Immune System Conferring DNA and RNA Virus Resistance in Plants Using CRISPR/Cas12a Multiplex Gene Editing

ABSTRACT Two types of CRISPR/Cas systems (Cas9 and Cas13) have been used to combat eukaryotic viruses successfully. In this study, we established resistance to the DNA virus BSCTV and RNA virus TMV in Nicotiana benthamiana using the CRISPR‐Cas12a multiplex gene editing system. We employed two effect...

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Bibliographic Details
Main Authors: Lili Luo, Liqing Miao, Xuhui Ma, Jinjin Hu, Suzhen Li, Wenzhu Yang, Shuai Ma, Rumei Chen, Xiaoqing Liu
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Plant Direct
Subjects:
BSCTV
CRISPR‐Cas12a
multiplex gene editing
TMV
virus resistance
Online Access:https://doi.org/10.1002/pld3.70070
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