Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis
Context: The lack of sensitivity and specificity of the existing diagnostic markers in Rheumatoid arthritis (RA) stimulates the search for new biomarkers to improve diagnostic sensitivity especially in seronegative cases. Aims: To estimate the levels of 14-3-3 η in RA cases, to assess the positivity...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-01-01
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| Series: | Indian Journal of Rheumatology |
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| Online Access: | http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2020;volume=15;issue=3;spage=175;epage=180;aulast=Yarlagadda |
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| author | Lakshmi Deepika Yarlagadda Rachel Jacob D Liza Rajasekhar Krishna Mohan Iyyapu Sai Baba S S. Kompella Vijaya Bhaskar Madrol N N Sreedevi Siraj Ahmed Khan Noorjahan Mohammed |
| author_facet | Lakshmi Deepika Yarlagadda Rachel Jacob D Liza Rajasekhar Krishna Mohan Iyyapu Sai Baba S S. Kompella Vijaya Bhaskar Madrol N N Sreedevi Siraj Ahmed Khan Noorjahan Mohammed |
| author_sort | Lakshmi Deepika Yarlagadda |
| collection | DOAJ |
| description | Context: The lack of sensitivity and specificity of the existing diagnostic markers in Rheumatoid arthritis (RA) stimulates the search for new biomarkers to improve diagnostic sensitivity especially in seronegative cases. Aims: To estimate the levels of 14-3-3 η in RA cases, to assess the positivity of 14-3-3 η in seronegative RA and to correlate rheumatoid factor (RF) positivity with 14-3-3 η positivity in seropositive RA. Settings and Design: This was cross sectional case control study by the Departments of Biochemistry and Rheumatology and clinical immunology. Subjects and Methods: A total of 61 RA cases and 20 healthy controls were included. Erythrocyte sedimentation rate, high sensitivity C reactive protein, RF, anti-citrullinated peptide antibodies (CCP) and 14-3-3 η protein were estimated. RA cases were further classified as seropositive (n = 23) and seronegative (n = 38) based on the positivity of either RF or anti-CCP result. Statistical Analysis: Prism 7 (GraphPad Software Inc.). Results: The median levels of 14-3-3 η (ng/ml) in cases (0.66 [0.2–1.1]) were significantly high compared to controls (0.1 [0.07–0.28]), P < 0.0001. It had a sensitivity of 74% and specificity of 90% at >0.3 ng/ml. 14-3-3 η showed statistically significant difference between seronegative cases and controls (P = 0.0003). The sensitivity and specificity of RF and anti-CCP were 33% and 85% and 37% and 90% respectively. The combination of 14-3-3 η, RF and anti-CCP showed sensitivity and specificity of 85.4% and 100% respectively with area under the curve 0.927. Conclusions: Our study demonstrated that serum levels of 14-3-3 η were significantly higher in cases compared to controls. Seronegative RA cases showed 14-3-3 η positivity in 71% of cases. 14-3-3 η appears to be a useful and highly specific marker in RA. |
| format | Article |
| id | doaj-art-c8ec00ab1d4047629f7e69e90617d5dc |
| institution | Kabale University |
| issn | 0973-3698 0973-3701 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Indian Journal of Rheumatology |
| spelling | doaj-art-c8ec00ab1d4047629f7e69e90617d5dc2025-02-03T10:51:12ZengSAGE PublishingIndian Journal of Rheumatology0973-36980973-37012020-01-0115317518010.4103/injr.injr_30_20Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritisLakshmi Deepika YarlagaddaRachel JacobD Liza RajasekharKrishna Mohan IyyapuSai Baba S S. KompellaVijaya Bhaskar MadrolN N SreedeviSiraj Ahmed KhanNoorjahan MohammedContext: The lack of sensitivity and specificity of the existing diagnostic markers in Rheumatoid arthritis (RA) stimulates the search for new biomarkers to improve diagnostic sensitivity especially in seronegative cases. Aims: To estimate the levels of 14-3-3 η in RA cases, to assess the positivity of 14-3-3 η in seronegative RA and to correlate rheumatoid factor (RF) positivity with 14-3-3 η positivity in seropositive RA. Settings and Design: This was cross sectional case control study by the Departments of Biochemistry and Rheumatology and clinical immunology. Subjects and Methods: A total of 61 RA cases and 20 healthy controls were included. Erythrocyte sedimentation rate, high sensitivity C reactive protein, RF, anti-citrullinated peptide antibodies (CCP) and 14-3-3 η protein were estimated. RA cases were further classified as seropositive (n = 23) and seronegative (n = 38) based on the positivity of either RF or anti-CCP result. Statistical Analysis: Prism 7 (GraphPad Software Inc.). Results: The median levels of 14-3-3 η (ng/ml) in cases (0.66 [0.2–1.1]) were significantly high compared to controls (0.1 [0.07–0.28]), P < 0.0001. It had a sensitivity of 74% and specificity of 90% at >0.3 ng/ml. 14-3-3 η showed statistically significant difference between seronegative cases and controls (P = 0.0003). The sensitivity and specificity of RF and anti-CCP were 33% and 85% and 37% and 90% respectively. The combination of 14-3-3 η, RF and anti-CCP showed sensitivity and specificity of 85.4% and 100% respectively with area under the curve 0.927. Conclusions: Our study demonstrated that serum levels of 14-3-3 η were significantly higher in cases compared to controls. Seronegative RA cases showed 14-3-3 η positivity in 71% of cases. 14-3-3 η appears to be a useful and highly specific marker in RA.http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2020;volume=15;issue=3;spage=175;epage=180;aulast=Yarlagaddaanti-citrullinated peptide antibodiesrheumatoid factorseronegative and seropositive |
| spellingShingle | Lakshmi Deepika Yarlagadda Rachel Jacob D Liza Rajasekhar Krishna Mohan Iyyapu Sai Baba S S. Kompella Vijaya Bhaskar Madrol N N Sreedevi Siraj Ahmed Khan Noorjahan Mohammed Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis Indian Journal of Rheumatology anti-citrullinated peptide antibodies rheumatoid factor seronegative and seropositive |
| title | Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis |
| title_full | Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis |
| title_fullStr | Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis |
| title_full_unstemmed | Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis |
| title_short | Evaluation of a new biomarker 14-3-3 Eta protein in diagnosis of rheumatoid arthritis |
| title_sort | evaluation of a new biomarker 14 3 3 eta protein in diagnosis of rheumatoid arthritis |
| topic | anti-citrullinated peptide antibodies rheumatoid factor seronegative and seropositive |
| url | http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2020;volume=15;issue=3;spage=175;epage=180;aulast=Yarlagadda |
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