Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering
In this paper, composite scaffolds consisting of sodium alginate (SA), nanoclay (NC) and hydroxyapatite (HA) were fabricated for articular cartilage tissue engineering using freeze-drying method. Central composite design (CCD)-based response surface methodology (RSM) was used to anticipate the mecha...
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| Format: | Article |
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001100 |
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| author | Padideh Shahbazi Majid Abdouss Mitra Naeimi Goldis Darbemamieh Milad Jafari-Nodoushan |
| author_facet | Padideh Shahbazi Majid Abdouss Mitra Naeimi Goldis Darbemamieh Milad Jafari-Nodoushan |
| author_sort | Padideh Shahbazi |
| collection | DOAJ |
| description | In this paper, composite scaffolds consisting of sodium alginate (SA), nanoclay (NC) and hydroxyapatite (HA) were fabricated for articular cartilage tissue engineering using freeze-drying method. Central composite design (CCD)-based response surface methodology (RSM) was used to anticipate the mechanical characteristics (in both compressive and tensile tests) and in vitro degradation of scaffolds with different amounts of SA and NC. The maximum tensile and compressive moduli (10.5 and 0.99 MPa, respectively) were found for the scaffold containing 2 g SA, 0.02 g NC and 0.2 g HA. In addition, it was found this composition had a relatively low degradation rate. Moreover, the optimized composition (S2N0.02) displayed a favorable porosity (50 %) and a proper swelling behavior. FTIR confirmed the successful integration of SA, NC and HA by presenting characteristic peaks corresponding to their functional groups, and SEM images showed the homogeneous dispersion of nanoparticles in the polymer matrix and the formation of interconnected pores suitable for cell seeding. Furthermore, MTT assay corroborated the good biocompatibility of the scaffolds for human chondrocytes. Drug release study showed that the scaffolds could effectively load glucosamine and provide its sustained release. These results confirm that SA/NC/HA composite scaffolds possess desirable properties for articular cartilage tissue engineering applications. |
| format | Article |
| id | doaj-art-c885ce3192434bf3b3473f9109e46fde |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-c885ce3192434bf3b3473f9109e46fde2025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010077110.1016/j.carpta.2025.100771Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineeringPadideh Shahbazi0Majid Abdouss1Mitra Naeimi2Goldis Darbemamieh3Milad Jafari-Nodoushan4Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Chemistry, Amirkabir University of Technology (AUT), Iran; Corresponding author.Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran; Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, IranHard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, IranIn this paper, composite scaffolds consisting of sodium alginate (SA), nanoclay (NC) and hydroxyapatite (HA) were fabricated for articular cartilage tissue engineering using freeze-drying method. Central composite design (CCD)-based response surface methodology (RSM) was used to anticipate the mechanical characteristics (in both compressive and tensile tests) and in vitro degradation of scaffolds with different amounts of SA and NC. The maximum tensile and compressive moduli (10.5 and 0.99 MPa, respectively) were found for the scaffold containing 2 g SA, 0.02 g NC and 0.2 g HA. In addition, it was found this composition had a relatively low degradation rate. Moreover, the optimized composition (S2N0.02) displayed a favorable porosity (50 %) and a proper swelling behavior. FTIR confirmed the successful integration of SA, NC and HA by presenting characteristic peaks corresponding to their functional groups, and SEM images showed the homogeneous dispersion of nanoparticles in the polymer matrix and the formation of interconnected pores suitable for cell seeding. Furthermore, MTT assay corroborated the good biocompatibility of the scaffolds for human chondrocytes. Drug release study showed that the scaffolds could effectively load glucosamine and provide its sustained release. These results confirm that SA/NC/HA composite scaffolds possess desirable properties for articular cartilage tissue engineering applications.http://www.sciencedirect.com/science/article/pii/S2666893925001100Composite scaffoldSodium alginateNanoclayHydroxyapatiteCartilage tissue engineering |
| spellingShingle | Padideh Shahbazi Majid Abdouss Mitra Naeimi Goldis Darbemamieh Milad Jafari-Nodoushan Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering Carbohydrate Polymer Technologies and Applications Composite scaffold Sodium alginate Nanoclay Hydroxyapatite Cartilage tissue engineering |
| title | Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| title_full | Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| title_fullStr | Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| title_full_unstemmed | Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| title_short | Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| title_sort | synthesis fabrication and characterization of composite scaffolds employing sodium alginate nanoclay and hydroxyapatite for articular cartilage tissue engineering |
| topic | Composite scaffold Sodium alginate Nanoclay Hydroxyapatite Cartilage tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001100 |
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