m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets

Abstract 5-Methylcytosine (m5C), an important RNA modification, has been extensively studied in colorectal cancer (CRC). In recent years, with advances in high-throughput sequencing technology and RNA modification detection tools, the role of m5C modification in tumorigenesis and development has bee...

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Main Authors: Jiawei Wang, Cenyi Qi, Ran Wang, Yuepeng Cao, Yuping Zhou, Guoliang Ye
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Journal of Translational Medicine
Subjects:
Online Access:https://doi.org/10.1186/s12967-025-06985-3
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author Jiawei Wang
Cenyi Qi
Ran Wang
Yuepeng Cao
Yuping Zhou
Guoliang Ye
author_facet Jiawei Wang
Cenyi Qi
Ran Wang
Yuepeng Cao
Yuping Zhou
Guoliang Ye
author_sort Jiawei Wang
collection DOAJ
description Abstract 5-Methylcytosine (m5C), an important RNA modification, has been extensively studied in colorectal cancer (CRC). In recent years, with advances in high-throughput sequencing technology and RNA modification detection tools, the role of m5C modification in tumorigenesis and development has been gradually elucidated and regulated by “writers,” “erasers,” and “readers,” m5C modification affects many biological processes and cancer progression, including cell proliferation, autophagy, invasion, and apoptosis. In CRC, m5C modification affects cancer cell proliferation, migration, invasion, drug resistance, and immunotherapy resistance by regulating RNA stability, metabolic reprogramming, signalling pathway activation, and immune escape. Moreover, m5C modification provides potential biomarkers and targets for early diagnosis and efficacy prediction. In addition, studies on drugs that target m5C modification related proteins have shown that intervention in m5C modification may provide new directions for immunotherapy and molecular therapy for patients with CRC. In summary, m5C modification, an important epigenetic regulators in CRC, provides a new perspective on the mechanism underlying cancer therapy and precision medicine research. Future research should focus on revealing the specific role of m5C modification in the tumor microenvironment and drug resistance mechanisms and promote its clinical translation in diagnosis and treatment.
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issn 1479-5876
language English
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publisher BMC
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spelling doaj-art-c8680ee280ef473882b04edfb285b51b2025-08-24T11:48:17ZengBMCJournal of Translational Medicine1479-58762025-08-0123111710.1186/s12967-025-06985-3m5C RNA modification in colorectal cancer: mechanisms and therapeutic targetsJiawei Wang0Cenyi Qi1Ran Wang2Yuepeng Cao3Yuping Zhou4Guoliang Ye5Department of Gastroenterology, The First Affiliated Hospital of Ningbo UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Ningbo UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Ningbo UniversityDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Ningbo UniversityAbstract 5-Methylcytosine (m5C), an important RNA modification, has been extensively studied in colorectal cancer (CRC). In recent years, with advances in high-throughput sequencing technology and RNA modification detection tools, the role of m5C modification in tumorigenesis and development has been gradually elucidated and regulated by “writers,” “erasers,” and “readers,” m5C modification affects many biological processes and cancer progression, including cell proliferation, autophagy, invasion, and apoptosis. In CRC, m5C modification affects cancer cell proliferation, migration, invasion, drug resistance, and immunotherapy resistance by regulating RNA stability, metabolic reprogramming, signalling pathway activation, and immune escape. Moreover, m5C modification provides potential biomarkers and targets for early diagnosis and efficacy prediction. In addition, studies on drugs that target m5C modification related proteins have shown that intervention in m5C modification may provide new directions for immunotherapy and molecular therapy for patients with CRC. In summary, m5C modification, an important epigenetic regulators in CRC, provides a new perspective on the mechanism underlying cancer therapy and precision medicine research. Future research should focus on revealing the specific role of m5C modification in the tumor microenvironment and drug resistance mechanisms and promote its clinical translation in diagnosis and treatment.https://doi.org/10.1186/s12967-025-06985-3Colorectal cancerRNA 5-methylcytosine (m5C)NSUN2DNMT2Early diagnosisTargeted therapy
spellingShingle Jiawei Wang
Cenyi Qi
Ran Wang
Yuepeng Cao
Yuping Zhou
Guoliang Ye
m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
Journal of Translational Medicine
Colorectal cancer
RNA 5-methylcytosine (m5C)
NSUN2
DNMT2
Early diagnosis
Targeted therapy
title m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
title_full m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
title_fullStr m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
title_full_unstemmed m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
title_short m5C RNA modification in colorectal cancer: mechanisms and therapeutic targets
title_sort m5c rna modification in colorectal cancer mechanisms and therapeutic targets
topic Colorectal cancer
RNA 5-methylcytosine (m5C)
NSUN2
DNMT2
Early diagnosis
Targeted therapy
url https://doi.org/10.1186/s12967-025-06985-3
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AT ranwang m5crnamodificationincolorectalcancermechanismsandtherapeutictargets
AT yuepengcao m5crnamodificationincolorectalcancermechanismsandtherapeutictargets
AT yupingzhou m5crnamodificationincolorectalcancermechanismsandtherapeutictargets
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