Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts
The objective of the present study was to compare recent methods for characterizing cell modifications. We studied the effect of extracellular reduced glutathione (GSH) on an olfactory neuroblast cell line (13s24). Three methods were used to monitor, in label-free, noninvasive real-time experiments,...
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2025-01-01
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| author | Alain Géloën Emmanuelle Berger |
| author_facet | Alain Géloën Emmanuelle Berger |
| author_sort | Alain Géloën |
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| description | The objective of the present study was to compare recent methods for characterizing cell modifications. We studied the effect of extracellular reduced glutathione (GSH) on an olfactory neuroblast cell line (13s24). Three methods were used to monitor, in label-free, noninvasive real-time experiments, cell surface occupancy by measuring impedance (xCELLigence), cell behavior (HoloMonitor cytometry), cell ultrastructure by measuring refractive index (3D Nanolive microscopy). Reduced glutathione dose-dependently increased cell volume and motility and decreased cell adhesion. Cell sorting analyses revealed that after short-term exposure (6 h), GSH reduced F-actin polymerization and extracellular glycoproteins leading to adhesion strength loss. Results support the hypothesis that excreted GSH could modulate disulfide bound-dependent integrin conformations involved in neurogenesis and/or neuronal plasticity. This is the first evidence of a causal link between GSH and changes in cell volume and motility required for cell division, migration, and/or differentiation. Results show the importance of real-time analysis methods, without labelling, in the study of cell responses under culture conditions. The present findings highlight important criteria in the choice of methods, beyond the parameters studied, such as cell preparation time, plate filling time, number of cells studied, friendly use of the devices, and the complexity of data processing. |
| format | Article |
| id | doaj-art-c85e61fa453e406cad3840a3146e67ad |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-c85e61fa453e406cad3840a3146e67ad2025-01-24T13:21:18ZengMDPI AGApplied Sciences2076-34172025-01-0115290810.3390/app15020908Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory NeuroblastsAlain Géloën0Emmanuelle Berger1Université de Lyon, UMR Ecologie Microbienne, CNRS 5557, INRA 1418, VetAgro Sup, Université Claude Bernard Lyon 1, Doua Campus, Dubois Bldg, 2nd Floor, 69622 Villeurbanne cedex, FranceUniversité de Lyon, UMR Ecologie Microbienne, CNRS 5557, INRA 1418, VetAgro Sup, Université Claude Bernard Lyon 1, Doua Campus, Dubois Bldg, 2nd Floor, 69622 Villeurbanne cedex, FranceThe objective of the present study was to compare recent methods for characterizing cell modifications. We studied the effect of extracellular reduced glutathione (GSH) on an olfactory neuroblast cell line (13s24). Three methods were used to monitor, in label-free, noninvasive real-time experiments, cell surface occupancy by measuring impedance (xCELLigence), cell behavior (HoloMonitor cytometry), cell ultrastructure by measuring refractive index (3D Nanolive microscopy). Reduced glutathione dose-dependently increased cell volume and motility and decreased cell adhesion. Cell sorting analyses revealed that after short-term exposure (6 h), GSH reduced F-actin polymerization and extracellular glycoproteins leading to adhesion strength loss. Results support the hypothesis that excreted GSH could modulate disulfide bound-dependent integrin conformations involved in neurogenesis and/or neuronal plasticity. This is the first evidence of a causal link between GSH and changes in cell volume and motility required for cell division, migration, and/or differentiation. Results show the importance of real-time analysis methods, without labelling, in the study of cell responses under culture conditions. The present findings highlight important criteria in the choice of methods, beyond the parameters studied, such as cell preparation time, plate filling time, number of cells studied, friendly use of the devices, and the complexity of data processing.https://www.mdpi.com/2076-3417/15/2/908olfactory neuroblast cell linecell volumecell adhesionreduced glutathioneHoloMonitorxCELLigence |
| spellingShingle | Alain Géloën Emmanuelle Berger Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts Applied Sciences olfactory neuroblast cell line cell volume cell adhesion reduced glutathione HoloMonitor xCELLigence |
| title | Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts |
| title_full | Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts |
| title_fullStr | Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts |
| title_full_unstemmed | Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts |
| title_short | Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts |
| title_sort | comparison of real time methods demonstrating the effects of reduced glutathione on olfactory neuroblasts |
| topic | olfactory neuroblast cell line cell volume cell adhesion reduced glutathione HoloMonitor xCELLigence |
| url | https://www.mdpi.com/2076-3417/15/2/908 |
| work_keys_str_mv | AT alaingeloen comparisonofrealtimemethodsdemonstratingtheeffectsofreducedglutathioneonolfactoryneuroblasts AT emmanuelleberger comparisonofrealtimemethodsdemonstratingtheeffectsofreducedglutathioneonolfactoryneuroblasts |