Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico
The blood bacterial microbiota of the Texas tortoise (Gopherus berlandieri) in Tamaulipas, Mexico, was characterized by next-generation sequencing. In 2019, blood was collected from 6 free-living tortoises. DNA was extracted, the V3-V4 region of the 16S rRNA gene was amplified, and Illumina sequen...
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| Format: | Article |
| Language: | English |
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Universidad Nacional Autónoma de México
2024-05-01
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| Series: | Revista Mexicana de Biodiversidad |
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| Online Access: | https://revista.ib.unam.mx/index.php/bio/article/view/5365 |
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| author | Eduardo Manolo Medrano-Zapata Jorge Luis Becerra-López Pedro Almaguer-Sierra Cristina García-De la Peña Ludivina Barrientos-Lozano Juan Flores-Gracia Karen Berenice Lara-Rodríguez Felipe Vaca-Paniagua Clara E. Díaz-Velásquez Aldo H. De la Cruz-Montoya |
| author_facet | Eduardo Manolo Medrano-Zapata Jorge Luis Becerra-López Pedro Almaguer-Sierra Cristina García-De la Peña Ludivina Barrientos-Lozano Juan Flores-Gracia Karen Berenice Lara-Rodríguez Felipe Vaca-Paniagua Clara E. Díaz-Velásquez Aldo H. De la Cruz-Montoya |
| author_sort | Eduardo Manolo Medrano-Zapata |
| collection | DOAJ |
| description |
The blood bacterial microbiota of the Texas tortoise (Gopherus berlandieri) in Tamaulipas, Mexico, was
characterized by next-generation sequencing. In 2019, blood was collected from 6 free-living tortoises. DNA was extracted, the V3-V4 region of the 16S rRNA gene was amplified, and Illumina sequencing was performed. The results showed 9 phyla, 20 classes, 42 orders, 81 families, 176 genera and 299 bacterial species. Firmicutes was the most abundant phylum in the blood of G. berlandieri; this taxon has been recorded as predominant in the intestine, excrement, nasal exudates and saliva of other species of the genus Gopherus. The dominant bacterial genera were Caldalkalibacillus, Anaerobacillus, Nesterenkonia and Bacillus. These taxa have been recorded in alkaline and halophilic soils, such as those found in G. berlandieri burrows. All of these bacterial taxa can enter the
bloodstream of G. berlandieri via intestinal, oral and nasal translocation. Likewise, 3 bacterial taxa (Coxiella sp., Ehrlichia sp. and Anaplasma phagocytophilum) that are transmitted by arthropod vectors, as well as the potentially pathogenic Salmonella enterica were recorded. This information is the first bacteriological reference for the blood of G. berlandieri, and is expected to be useful for health and conservation programs.
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| format | Article |
| id | doaj-art-c83e3eaebc5d48a7b048f948c51a6e6d |
| institution | Kabale University |
| issn | 1870-3453 2007-8706 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Universidad Nacional Autónoma de México |
| record_format | Article |
| series | Revista Mexicana de Biodiversidad |
| spelling | doaj-art-c83e3eaebc5d48a7b048f948c51a6e6d2025-08-20T03:52:57ZengUniversidad Nacional Autónoma de MéxicoRevista Mexicana de Biodiversidad1870-34532007-87062024-05-019510.22201/ib.20078706e.2024.95.5365Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, MexicoEduardo Manolo Medrano-Zapata0Jorge Luis Becerra-López1Pedro Almaguer-Sierra2Cristina García-De la Peña3Ludivina Barrientos-Lozano4Juan Flores-Gracia5Karen Berenice Lara-Rodríguez6Felipe Vaca-Paniagua7Clara E. Díaz-Velásquez8Aldo H. De la Cruz-Montoya9Tecnológico Nacional de MéxicoUniversidad Juárez del Estado de DurangoTecnológico Nacional de MéxicoUniversidad Juárez del Estado de DurangoTecnológico Nacional de MéxicoTecnológico Nacional de MéxicoTecnológico Nacional de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de México The blood bacterial microbiota of the Texas tortoise (Gopherus berlandieri) in Tamaulipas, Mexico, was characterized by next-generation sequencing. In 2019, blood was collected from 6 free-living tortoises. DNA was extracted, the V3-V4 region of the 16S rRNA gene was amplified, and Illumina sequencing was performed. The results showed 9 phyla, 20 classes, 42 orders, 81 families, 176 genera and 299 bacterial species. Firmicutes was the most abundant phylum in the blood of G. berlandieri; this taxon has been recorded as predominant in the intestine, excrement, nasal exudates and saliva of other species of the genus Gopherus. The dominant bacterial genera were Caldalkalibacillus, Anaerobacillus, Nesterenkonia and Bacillus. These taxa have been recorded in alkaline and halophilic soils, such as those found in G. berlandieri burrows. All of these bacterial taxa can enter the bloodstream of G. berlandieri via intestinal, oral and nasal translocation. Likewise, 3 bacterial taxa (Coxiella sp., Ehrlichia sp. and Anaplasma phagocytophilum) that are transmitted by arthropod vectors, as well as the potentially pathogenic Salmonella enterica were recorded. This information is the first bacteriological reference for the blood of G. berlandieri, and is expected to be useful for health and conservation programs. https://revista.ib.unam.mx/index.php/bio/article/view/5365SequencingCoxiellaEhrlichiaAnaplasma phagocytophilumSalmonella enterica |
| spellingShingle | Eduardo Manolo Medrano-Zapata Jorge Luis Becerra-López Pedro Almaguer-Sierra Cristina García-De la Peña Ludivina Barrientos-Lozano Juan Flores-Gracia Karen Berenice Lara-Rodríguez Felipe Vaca-Paniagua Clara E. Díaz-Velásquez Aldo H. De la Cruz-Montoya Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico Revista Mexicana de Biodiversidad Sequencing Coxiella Ehrlichia Anaplasma phagocytophilum Salmonella enterica |
| title | Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico |
| title_full | Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico |
| title_fullStr | Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico |
| title_full_unstemmed | Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico |
| title_short | Blood bacterial microbiota of the Texas tortoise, Gopherus berlandieri, in Tamaulipas, Mexico |
| title_sort | blood bacterial microbiota of the texas tortoise gopherus berlandieri in tamaulipas mexico |
| topic | Sequencing Coxiella Ehrlichia Anaplasma phagocytophilum Salmonella enterica |
| url | https://revista.ib.unam.mx/index.php/bio/article/view/5365 |
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