The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil

Studies on the influence of the rhizosphere on the growth of Sphingo- monas chlorophenolica during. Pentacholophenol (PCP) degradation in batch culture and in soil were carried out. In batch culture, a basal minimal medium with or without rhizosphere exudates extracted from winter wheat was used. In...

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Main Authors: Rosemeri Inês Dams, Graeme Paton, Ken Killhan
Format: Article
Language:English
Published: Universidade Federal de Santa Catarina 2006-01-01
Series:Biotemas
Online Access:https://periodicos.ufsc.br/index.php/biotemas/article/view/21089
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author Rosemeri Inês Dams
Graeme Paton
Ken Killhan
author_facet Rosemeri Inês Dams
Graeme Paton
Ken Killhan
author_sort Rosemeri Inês Dams
collection DOAJ
description Studies on the influence of the rhizosphere on the growth of Sphingo- monas chlorophenolica during. Pentacholophenol (PCP) degradation in batch culture and in soil were carried out. In batch culture, a basal minimal medium with or without rhizosphere exudates extracted from winter wheat was used. In soil systems, degradation experiments were performed in the presence and absence of plants. Measurements of PCP concentrations were made using high performance liquid chroma- tography analysis (HPLC). Bacterial analyses of S. chlorophenolica were carried out by plating on MSM medium. The results showed that the rhizosphere exudates stimulated the growth of the cells of S. chlorophe- nolica at concentrations of 50 and 80mg kg dry wt soil –1 as well as stimu- lating the ability of S. chlorophenolica to degrade PCP at a concentration of 80mg Kg dry wt soil -1. In addition, pentachlorophenol had an adverse effect on the growth of S. chlorophenolica. The introduction of S. chlorop- henolica into the loamy soil with plants showed a faster degradation when compared to the inocu- lated soil without plants. There was a significant increase of S. chloroph- enolica in the roots in comparison to those in thesoil. This study showed that the presence of the inoculum S. chlorophe nolica enhanced the PCP degradation in a loamy soil and it indicates the potential for a treatment process under a appropriate environmental conditions such as there present in soil systems.
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spelling doaj-art-c83e01917bcc4ab89dcad9fe11c8f69d2025-08-20T03:44:18ZengUniversidade Federal de Santa CatarinaBiotemas0103-16432175-79252006-01-01194The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soilRosemeri Inês Dams0Graeme Paton1Ken Killhan2UNIVALI – Laboratório de Microbiologia de Alimentos. Universidade do Vale do Itajaí 5ª avenida s/n, Bairro dos Municípios, CEP: 88330-000, Balneário Camboriú – SCDepartment of Plant and Soil Science, University of Aberdeen, United KingdomDepartment of Plant and Soil Science, University of Aberdeen, United KingdomStudies on the influence of the rhizosphere on the growth of Sphingo- monas chlorophenolica during. Pentacholophenol (PCP) degradation in batch culture and in soil were carried out. In batch culture, a basal minimal medium with or without rhizosphere exudates extracted from winter wheat was used. In soil systems, degradation experiments were performed in the presence and absence of plants. Measurements of PCP concentrations were made using high performance liquid chroma- tography analysis (HPLC). Bacterial analyses of S. chlorophenolica were carried out by plating on MSM medium. The results showed that the rhizosphere exudates stimulated the growth of the cells of S. chlorophe- nolica at concentrations of 50 and 80mg kg dry wt soil –1 as well as stimu- lating the ability of S. chlorophenolica to degrade PCP at a concentration of 80mg Kg dry wt soil -1. In addition, pentachlorophenol had an adverse effect on the growth of S. chlorophenolica. The introduction of S. chlorop- henolica into the loamy soil with plants showed a faster degradation when compared to the inocu- lated soil without plants. There was a significant increase of S. chloroph- enolica in the roots in comparison to those in thesoil. This study showed that the presence of the inoculum S. chlorophe nolica enhanced the PCP degradation in a loamy soil and it indicates the potential for a treatment process under a appropriate environmental conditions such as there present in soil systems.https://periodicos.ufsc.br/index.php/biotemas/article/view/21089
spellingShingle Rosemeri Inês Dams
Graeme Paton
Ken Killhan
The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
Biotemas
title The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
title_full The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
title_fullStr The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
title_full_unstemmed The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
title_short The effect of rhizosphere on growth of Sphingomonas chlorophenolica ATCC 39723 during pentachlorophenol (PCP) biodegradation in batch culture and soil
title_sort effect of rhizosphere on growth of sphingomonas chlorophenolica atcc 39723 during pentachlorophenol pcp biodegradation in batch culture and soil
url https://periodicos.ufsc.br/index.php/biotemas/article/view/21089
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