Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy
<b>Background:</b> In dogs, bacterial urinary tract infections are a frequent cause of antimicrobial prescription, increasing the risk of selecting antibiotic-resistant bacteria. This study analyzed resistance patterns, the presence of extended-spectrum β-lactamases (ESBLs) and biofilm-f...
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2025-01-01
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| author | Alessia Facchin Joel Filipe Irene Mauri Filippo Tagliasacchi Guido Grilli Tiziana Vitiello Gabriele Ratti Laura Musa Martina Penati Paola Scarpa Stefania Lauzi |
| author_facet | Alessia Facchin Joel Filipe Irene Mauri Filippo Tagliasacchi Guido Grilli Tiziana Vitiello Gabriele Ratti Laura Musa Martina Penati Paola Scarpa Stefania Lauzi |
| author_sort | Alessia Facchin |
| collection | DOAJ |
| description | <b>Background:</b> In dogs, bacterial urinary tract infections are a frequent cause of antimicrobial prescription, increasing the risk of selecting antibiotic-resistant bacteria. This study analyzed resistance patterns, the presence of extended-spectrum β-lactamases (ESBLs) and biofilm-forming capacity in <i>E. coli</i> and <i>K. pneumoniae</i> previously isolated from urine samples collected from 133 selected dogs admitted to the Veterinary Teaching Hospital of Milan, Italy, in 2021 and 2023. <b>Methods</b>: The <i>E. coli</i> and <i>K. pneumoniae</i> isolates were bacteriologically and genetically analyzed. <b>Results</b>: Overall, 53/133 (39.8%) samples had a positive microbiological culture. Thirty-four <i>E. coli</i>/<i>K. pneumoniae</i> isolates were detected, accounting for 26.5% of the examined samples. The 34 isolates included 28 <i>E. coli</i> and 6 <i>K. pneumoniae</i>. Four (11.8%) were ESBL-producing bacteria, all supported by <i>bla</i><sub>CTX-M</sub> gene belonging to group 1. The <i>K. pneumoniae</i> isolates were significantly associated with ESBL production (<i>p</i> < 0.05). MIC analysis showed 11 (32.4%) multidrug-resistant isolates. Biofilm-forming capacity was observed in 23 (67.6%) isolates, regardless of bacterial species, including 20 weakly and 3 moderately adherent bacteria. All moderate biofilm producers were <i>K. pneumoniae</i>. Multidrug resistance (MDR) was significantly more present in strains with moderate biofilm-forming ability compared to strains with weak ability to form biofilm (<i>p</i> < 0.05). <i>E. coli</i> was confirmed as the most commonly identified urinary isolate in dogs. <b>Conclusions</b>: The high presence of ESBL producers and MDR in <i>K. pneumoniae</i> suggests mandatory in vitro susceptibility testing in the presence of this bacterium in dogs with UTI. The association of moderate biofilm production with MDR highlights the need for monitoring and surveillance of bacterial prevalence and resistance patterns of urinary isolates in dogs. |
| format | Article |
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| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-c83c38cabfe24583bb19ff88841350202025-01-24T13:18:37ZengMDPI AGAntibiotics2079-63822025-01-011413110.3390/antibiotics14010031Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from ItalyAlessia Facchin0Joel Filipe1Irene Mauri2Filippo Tagliasacchi3Guido Grilli4Tiziana Vitiello5Gabriele Ratti6Laura Musa7Martina Penati8Paola Scarpa9Stefania Lauzi10Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, Italy<b>Background:</b> In dogs, bacterial urinary tract infections are a frequent cause of antimicrobial prescription, increasing the risk of selecting antibiotic-resistant bacteria. This study analyzed resistance patterns, the presence of extended-spectrum β-lactamases (ESBLs) and biofilm-forming capacity in <i>E. coli</i> and <i>K. pneumoniae</i> previously isolated from urine samples collected from 133 selected dogs admitted to the Veterinary Teaching Hospital of Milan, Italy, in 2021 and 2023. <b>Methods</b>: The <i>E. coli</i> and <i>K. pneumoniae</i> isolates were bacteriologically and genetically analyzed. <b>Results</b>: Overall, 53/133 (39.8%) samples had a positive microbiological culture. Thirty-four <i>E. coli</i>/<i>K. pneumoniae</i> isolates were detected, accounting for 26.5% of the examined samples. The 34 isolates included 28 <i>E. coli</i> and 6 <i>K. pneumoniae</i>. Four (11.8%) were ESBL-producing bacteria, all supported by <i>bla</i><sub>CTX-M</sub> gene belonging to group 1. The <i>K. pneumoniae</i> isolates were significantly associated with ESBL production (<i>p</i> < 0.05). MIC analysis showed 11 (32.4%) multidrug-resistant isolates. Biofilm-forming capacity was observed in 23 (67.6%) isolates, regardless of bacterial species, including 20 weakly and 3 moderately adherent bacteria. All moderate biofilm producers were <i>K. pneumoniae</i>. Multidrug resistance (MDR) was significantly more present in strains with moderate biofilm-forming ability compared to strains with weak ability to form biofilm (<i>p</i> < 0.05). <i>E. coli</i> was confirmed as the most commonly identified urinary isolate in dogs. <b>Conclusions</b>: The high presence of ESBL producers and MDR in <i>K. pneumoniae</i> suggests mandatory in vitro susceptibility testing in the presence of this bacterium in dogs with UTI. The association of moderate biofilm production with MDR highlights the need for monitoring and surveillance of bacterial prevalence and resistance patterns of urinary isolates in dogs.https://www.mdpi.com/2079-6382/14/1/31doguropathogenUTI<i>E. coli</i><i>K. pneumoniae</i> |
| spellingShingle | Alessia Facchin Joel Filipe Irene Mauri Filippo Tagliasacchi Guido Grilli Tiziana Vitiello Gabriele Ratti Laura Musa Martina Penati Paola Scarpa Stefania Lauzi Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy Antibiotics dog uropathogen UTI <i>E. coli</i> <i>K. pneumoniae</i> |
| title | Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy |
| title_full | Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy |
| title_fullStr | Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy |
| title_full_unstemmed | Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy |
| title_short | Antimicrobial Resistance and Biofilm-Forming Ability in ESBL-Producing and Non-ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Canine Urinary Samples from Italy |
| title_sort | antimicrobial resistance and biofilm forming ability in esbl producing and non esbl producing i escherichia coli i and i klebsiella pneumoniae i isolated from canine urinary samples from italy |
| topic | dog uropathogen UTI <i>E. coli</i> <i>K. pneumoniae</i> |
| url | https://www.mdpi.com/2079-6382/14/1/31 |
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