MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy
Background. Diabetes mellitus is the leading cause of end-stage renal disease worldwide. Microalbuminuria is the cornerstone for the diagnosis of diabetic nephropathy. However, it is an inadequate marker for early diagnosis. MicroRNAs are not only new and promising markers for early diagnosis but al...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Nephrology |
| Online Access: | http://dx.doi.org/10.1155/2020/8075376 |
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| author | Mostafa Abdelsalam A. M. Wahab Maysaa El Sayed Zaki Mohamad Motawea |
| author_facet | Mostafa Abdelsalam A. M. Wahab Maysaa El Sayed Zaki Mohamad Motawea |
| author_sort | Mostafa Abdelsalam |
| collection | DOAJ |
| description | Background. Diabetes mellitus is the leading cause of end-stage renal disease worldwide. Microalbuminuria is the cornerstone for the diagnosis of diabetic nephropathy. However, it is an inadequate marker for early diagnosis. MicroRNAs are not only new and promising markers for early diagnosis but also, but they may also play a role in the prevention of disease progression. Methods. This study included ninety patients with type 2 DM in addition to 30 control subjects. MicroRNA-451 expression in blood and plasma using real-time PCR was evaluated in addition to the classic diabetic nephropathy markers (serum creatinine, urinary albumin, and eGFR). Results. There was a significant difference between the studied groups versus control regarding serum creatinine, eGFR, urinary, and plasma microRNA-451 with p=0.0001. Patients with eGFR 60 ml/min/1.73 m2 showed a significantly higher plasma microRNA-451 (29.6 ± 1.6) and significantly lower urinary microRNA-451 (21 ± 0.9) in comparison to patients with eGFR >60 ml/min/1.73 m2 and p=0.0001. eGFR showed a positive correlation with urinary microRNA-451 and negative correlation with both plasma microRNA-451 and urinary albumin. Both plasma and urinary microRNA-451 are highly sensitive and specific markers for chronicity in diabetic nephropathy patients with sensitivity of 90.9% and 95.5% and specificity of 67.6% and 95.6%, respectively. Conclusion. MicroRNA-451 is a promising early biomarker for chronic kidney disease in diabetic nephropathy with high sensitivity and specificity. |
| format | Article |
| id | doaj-art-c82c60400cd049eebf1c63b17fb677b7 |
| institution | Kabale University |
| issn | 2090-214X 2090-2158 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Nephrology |
| spelling | doaj-art-c82c60400cd049eebf1c63b17fb677b72025-02-03T01:04:06ZengWileyInternational Journal of Nephrology2090-214X2090-21582020-01-01202010.1155/2020/80753768075376MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic NephropathyMostafa Abdelsalam0A. M. Wahab1Maysaa El Sayed Zaki2Mohamad Motawea3Mansoura Nephrology and Dialysis Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, EgyptMansoura Nephrology and Dialysis Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, EgyptClinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, EgyptEndocrinology Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, EgyptBackground. Diabetes mellitus is the leading cause of end-stage renal disease worldwide. Microalbuminuria is the cornerstone for the diagnosis of diabetic nephropathy. However, it is an inadequate marker for early diagnosis. MicroRNAs are not only new and promising markers for early diagnosis but also, but they may also play a role in the prevention of disease progression. Methods. This study included ninety patients with type 2 DM in addition to 30 control subjects. MicroRNA-451 expression in blood and plasma using real-time PCR was evaluated in addition to the classic diabetic nephropathy markers (serum creatinine, urinary albumin, and eGFR). Results. There was a significant difference between the studied groups versus control regarding serum creatinine, eGFR, urinary, and plasma microRNA-451 with p=0.0001. Patients with eGFR 60 ml/min/1.73 m2 showed a significantly higher plasma microRNA-451 (29.6 ± 1.6) and significantly lower urinary microRNA-451 (21 ± 0.9) in comparison to patients with eGFR >60 ml/min/1.73 m2 and p=0.0001. eGFR showed a positive correlation with urinary microRNA-451 and negative correlation with both plasma microRNA-451 and urinary albumin. Both plasma and urinary microRNA-451 are highly sensitive and specific markers for chronicity in diabetic nephropathy patients with sensitivity of 90.9% and 95.5% and specificity of 67.6% and 95.6%, respectively. Conclusion. MicroRNA-451 is a promising early biomarker for chronic kidney disease in diabetic nephropathy with high sensitivity and specificity.http://dx.doi.org/10.1155/2020/8075376 |
| spellingShingle | Mostafa Abdelsalam A. M. Wahab Maysaa El Sayed Zaki Mohamad Motawea MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy International Journal of Nephrology |
| title | MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy |
| title_full | MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy |
| title_fullStr | MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy |
| title_full_unstemmed | MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy |
| title_short | MicroRNA-451 as an Early Predictor of Chronic Kidney Disease in Diabetic Nephropathy |
| title_sort | microrna 451 as an early predictor of chronic kidney disease in diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2020/8075376 |
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