A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>)
Aromatase inhibitor letrozole (LZ) and 17α-methyltestosterone (MT) are commonly used for sex control breeding to induce female-to-male sex reversal in aquaculture. However, studies on the molecular regulatory mechanisms of LZ and MT are still limited. Here, a comparative transcriptome analysis was c...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/10/1/21 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588510309122048 |
|---|---|
| author | Qi Zhou Yan Wang Yuwen Guo Guangli Li Tao Wang Huapu Chen |
| author_facet | Qi Zhou Yan Wang Yuwen Guo Guangli Li Tao Wang Huapu Chen |
| author_sort | Qi Zhou |
| collection | DOAJ |
| description | Aromatase inhibitor letrozole (LZ) and 17α-methyltestosterone (MT) are commonly used for sex control breeding to induce female-to-male sex reversal in aquaculture. However, studies on the molecular regulatory mechanisms of LZ and MT are still limited. Here, a comparative transcriptome analysis was conducted on the hypothalamus, the gonad, and the liver of golden pompano (<i>Trachinotus ovatus</i>) after 30 days of LZ and MT treatment, which demonstrated significant sexual size dimorphism. Following LZ treatment, the transcriptome data identified 156, 890, and 1286 different expression genes (DEGs) in the hypothalamus, the liver, and the gonad, respectively. After MT treatment, the transcriptome data identified 114, 876, and 950 DEGs in the hypothalamus, the liver, and the gonad, respectively. Functional enrichment analysis revealed that DEGs were involved in oocyte meiosis and steroid-hormone synthesis following LZ treatment. Similarly, the analysis indicated that DEGs were associated with cysteine and methionine metabolism and steroid-hormone synthesis after MT treatment. Further analysis showed that LZ treatment can promote the first meiotic division but inhibit the second meiotic division of the oocyte. MT treatment promotes polyamine synthesis to induce oocyte apoptosis, both of which affect the trajectory of ovarian differentiation in golden pompano. However, LZ and MT have converse effects in sex hormone synthesis. LZ inhibits estrogen metabolism but promotes cholesterol synthesis by regulating <i>sqle</i>, <i>cyp3a40</i>, <i>ugt1a1</i>, and <i>ugt2a1</i>, while MT promotes estrogen metabolism but inhibits the conversion of cholesterol to pregnenolone by regulating <i>cyp19a1</i>, <i>cyp1a</i>, and <i>hsd17b7</i>. This study reveals that both LZ and MT may inhibit the ovarian differentiation and induce masculinization through different regulatory mechanisms, which provides a fundamental insight into the sex control breeding in aquaculture. |
| format | Article |
| id | doaj-art-c826624d21e149e08f4c505d3d6c8789 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-c826624d21e149e08f4c505d3d6c87892025-01-24T13:32:28ZengMDPI AGFishes2410-38882025-01-011012110.3390/fishes10010021A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>)Qi Zhou0Yan Wang1Yuwen Guo2Guangli Li3Tao Wang4Huapu Chen5Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaAromatase inhibitor letrozole (LZ) and 17α-methyltestosterone (MT) are commonly used for sex control breeding to induce female-to-male sex reversal in aquaculture. However, studies on the molecular regulatory mechanisms of LZ and MT are still limited. Here, a comparative transcriptome analysis was conducted on the hypothalamus, the gonad, and the liver of golden pompano (<i>Trachinotus ovatus</i>) after 30 days of LZ and MT treatment, which demonstrated significant sexual size dimorphism. Following LZ treatment, the transcriptome data identified 156, 890, and 1286 different expression genes (DEGs) in the hypothalamus, the liver, and the gonad, respectively. After MT treatment, the transcriptome data identified 114, 876, and 950 DEGs in the hypothalamus, the liver, and the gonad, respectively. Functional enrichment analysis revealed that DEGs were involved in oocyte meiosis and steroid-hormone synthesis following LZ treatment. Similarly, the analysis indicated that DEGs were associated with cysteine and methionine metabolism and steroid-hormone synthesis after MT treatment. Further analysis showed that LZ treatment can promote the first meiotic division but inhibit the second meiotic division of the oocyte. MT treatment promotes polyamine synthesis to induce oocyte apoptosis, both of which affect the trajectory of ovarian differentiation in golden pompano. However, LZ and MT have converse effects in sex hormone synthesis. LZ inhibits estrogen metabolism but promotes cholesterol synthesis by regulating <i>sqle</i>, <i>cyp3a40</i>, <i>ugt1a1</i>, and <i>ugt2a1</i>, while MT promotes estrogen metabolism but inhibits the conversion of cholesterol to pregnenolone by regulating <i>cyp19a1</i>, <i>cyp1a</i>, and <i>hsd17b7</i>. This study reveals that both LZ and MT may inhibit the ovarian differentiation and induce masculinization through different regulatory mechanisms, which provides a fundamental insight into the sex control breeding in aquaculture.https://www.mdpi.com/2410-3888/10/1/21<i>Trachinotus ovatus</i>transcriptomeletrozole17α-methyltestosterone |
| spellingShingle | Qi Zhou Yan Wang Yuwen Guo Guangli Li Tao Wang Huapu Chen A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) Fishes <i>Trachinotus ovatus</i> transcriptome letrozole 17α-methyltestosterone |
| title | A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_full | A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_fullStr | A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_full_unstemmed | A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_short | A Comparative Transcriptome Analysis Reveals the Effects of Letrozole and 17α-Methyltestosterone on the Expression of Reproductive-Related Genes in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_sort | comparative transcriptome analysis reveals the effects of letrozole and 17α methyltestosterone on the expression of reproductive related genes in golden pompano i trachinotus ovatus i |
| topic | <i>Trachinotus ovatus</i> transcriptome letrozole 17α-methyltestosterone |
| url | https://www.mdpi.com/2410-3888/10/1/21 |
| work_keys_str_mv | AT qizhou acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT yanwang acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT yuwenguo acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT guanglili acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT taowang acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT huapuchen acomparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT qizhou comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT yanwang comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT yuwenguo comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT guanglili comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT taowang comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi AT huapuchen comparativetranscriptomeanalysisrevealstheeffectsofletrozoleand17amethyltestosteroneontheexpressionofreproductiverelatedgenesingoldenpompanoitrachinotusovatusi |