Development and application of reverse transcriptase droplet digital PCR technology for sensitive detection of BVDV-1 and BTV in bovine semen

Development and application of reverse transcriptase droplet digital PCR technology for sensitive detection of BVDV-1 and BTV in bovine semen

Abstract Background Bovine viral diarrhoea virus genotype 1 (BVDV-1) and bluetongue virus (BTV) are potent viral pathogens that may be transmitted through semen, resulting in the spread of diseases via artificial insemination. Thus, establishing an early detection method for BVDV-1 and BTV infection...

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Bibliographic Details
Main Authors: Zhichao Yu, Linjun Chen, Qiang Cui, Han Yan, Junyan Li, Xiaoping Luo, Yingying Li, Xianghong Ju, Yanhong Yong, Namula Zhao, Zhiguo Zhao
Format: Article
Language:English
Published: BMC 2025-01-01
Series:BMC Veterinary Research
Subjects:
Bovine viral diarrhoea virus genotype 1
Bluetongue virus
Droplet digital PCR
Bovine semen
Online Access:https://doi.org/10.1186/s12917-025-04506-4
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