Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products
To study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2018/5136185 |
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| author | Jian-Min Wang Xin-Yuan Sun Jian-Ming Ouyang |
| author_facet | Jian-Min Wang Xin-Yuan Sun Jian-Ming Ouyang |
| author_sort | Jian-Min Wang |
| collection | DOAJ |
| description | To study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8.38 (APS1), 4.72 (APS2), and 2.60 kDa (APS3). The structures of these polysaccharides were characterized by 1H NMR, 13C NMR, FT-IR, and GC/MS. The degradation process did not cause significant changes in the main chain structure of APS. The monosaccharide component of APS before and after degradation was slightly changed. The antioxidant ability in vitro (removing hydroxyl and ABTS radicals and reducing ability) and in cells (superoxide dismutase and malondialdehyde generation) of these polysaccharides is closely related to their molecular weight. If the molecular weight of APS is very high or low, it is not conducive to their activity. Only APS2 with moderate molecular weight showed the greatest antioxidant activity and ability to repair human kidney epithelial (HK-2) cells. Therefore, APS2 can be used as a potential antistone polysaccharide drug. |
| format | Article |
| id | doaj-art-c8098fd41f684c81a8b36aa44746fa18 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | International Journal of Polymer Science |
| spelling | doaj-art-c8098fd41f684c81a8b36aa44746fa182025-02-03T06:12:35ZengWileyInternational Journal of Polymer Science1687-94221687-94302018-01-01201810.1155/2018/51361855136185Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation ProductsJian-Min Wang0Xin-Yuan Sun1Jian-Ming Ouyang2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaInstitute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaInstitute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaTo study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8.38 (APS1), 4.72 (APS2), and 2.60 kDa (APS3). The structures of these polysaccharides were characterized by 1H NMR, 13C NMR, FT-IR, and GC/MS. The degradation process did not cause significant changes in the main chain structure of APS. The monosaccharide component of APS before and after degradation was slightly changed. The antioxidant ability in vitro (removing hydroxyl and ABTS radicals and reducing ability) and in cells (superoxide dismutase and malondialdehyde generation) of these polysaccharides is closely related to their molecular weight. If the molecular weight of APS is very high or low, it is not conducive to their activity. Only APS2 with moderate molecular weight showed the greatest antioxidant activity and ability to repair human kidney epithelial (HK-2) cells. Therefore, APS2 can be used as a potential antistone polysaccharide drug.http://dx.doi.org/10.1155/2018/5136185 |
| spellingShingle | Jian-Min Wang Xin-Yuan Sun Jian-Ming Ouyang Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products International Journal of Polymer Science |
| title | Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products |
| title_full | Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products |
| title_fullStr | Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products |
| title_full_unstemmed | Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products |
| title_short | Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products |
| title_sort | structural characterization antioxidant activity and biomedical application of astragalus polysaccharide degradation products |
| url | http://dx.doi.org/10.1155/2018/5136185 |
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