The gut microbiota predicts and time-restricted feeding delays experimental colitis
The etiology of inflammatory bowel disease (IBD) remains unclear, treatment options unsatisfactory and disease development difficult to predict for individual patients. Dysbiosis of the gastrointestinal microbiota and disruption of the biological clock have been implicated and studied as diagnostic...
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2025.2453019 |
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| author | Hannah K. Ruple Eva Haasis Anna Bettenburg Carina Maier Carolin Fritz Laura Schüle Sarah Löcker Yvonne Soltow Lynn Schintgen Nina S. Schmidt Celine Schneider Axel Lorentz W. Florian Fricke |
| author_facet | Hannah K. Ruple Eva Haasis Anna Bettenburg Carina Maier Carolin Fritz Laura Schüle Sarah Löcker Yvonne Soltow Lynn Schintgen Nina S. Schmidt Celine Schneider Axel Lorentz W. Florian Fricke |
| author_sort | Hannah K. Ruple |
| collection | DOAJ |
| description | The etiology of inflammatory bowel disease (IBD) remains unclear, treatment options unsatisfactory and disease development difficult to predict for individual patients. Dysbiosis of the gastrointestinal microbiota and disruption of the biological clock have been implicated and studied as diagnostic and therapeutic targets. Here, we examine the relationship of IBD to biological clock and gut microbiota by using the IL-10 deficient (IL-10-/-) mouse model for microbiota-dependent spontaneous colitis in combination with altered (4 h/4 h) light/dark cycles to disrupt and time-restricted feeding (TRF) to restore circadian rhythmicity. We show that while altered light/dark cycles disrupted the intestinal clock in wild type (WT) mice, IL-10-/- mice were characterized by altered microbiota composition, impaired intestinal clock, and microbiota rhythmicity irrespective of external clock disruption, which had no consistent colitis-promoting effect on IL-10-/- mice. TRF delayed colitis onset reduced the expression of inflammatory markers and increased the expression of clock genes in the intestine, and increased gut microbiota rhythmicity in IL-10-/- mice. Compositional changes and reduced rhythmicity of the fecal microbiota preceded colitis and could predict colitis symptoms for individual IL-10-/- mice across different experiments. Our findings provide perspectives for new diagnostic and TRF-based, therapeutic applications in IBD that should be further explored. |
| format | Article |
| id | doaj-art-c7d2f92fd6104ab5a6995a7057f3a98f |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-c7d2f92fd6104ab5a6995a7057f3a98f2025-01-26T16:21:44ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2453019The gut microbiota predicts and time-restricted feeding delays experimental colitisHannah K. Ruple0Eva Haasis1Anna Bettenburg2Carina Maier3Carolin Fritz4Laura Schüle5Sarah Löcker6Yvonne Soltow7Lynn Schintgen8Nina S. Schmidt9Celine Schneider10Axel Lorentz11W. Florian Fricke12Department of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyInstitute of Nutritional Medicine, University of Hohenheim, Stuttgart, GermanyDepartment of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, GermanyThe etiology of inflammatory bowel disease (IBD) remains unclear, treatment options unsatisfactory and disease development difficult to predict for individual patients. Dysbiosis of the gastrointestinal microbiota and disruption of the biological clock have been implicated and studied as diagnostic and therapeutic targets. Here, we examine the relationship of IBD to biological clock and gut microbiota by using the IL-10 deficient (IL-10-/-) mouse model for microbiota-dependent spontaneous colitis in combination with altered (4 h/4 h) light/dark cycles to disrupt and time-restricted feeding (TRF) to restore circadian rhythmicity. We show that while altered light/dark cycles disrupted the intestinal clock in wild type (WT) mice, IL-10-/- mice were characterized by altered microbiota composition, impaired intestinal clock, and microbiota rhythmicity irrespective of external clock disruption, which had no consistent colitis-promoting effect on IL-10-/- mice. TRF delayed colitis onset reduced the expression of inflammatory markers and increased the expression of clock genes in the intestine, and increased gut microbiota rhythmicity in IL-10-/- mice. Compositional changes and reduced rhythmicity of the fecal microbiota preceded colitis and could predict colitis symptoms for individual IL-10-/- mice across different experiments. Our findings provide perspectives for new diagnostic and TRF-based, therapeutic applications in IBD that should be further explored.https://www.tandfonline.com/doi/10.1080/19490976.2025.2453019Biological clockgut microbiotacircadian rhythmtime-restricted feedingexperimental colitisIL-10 |
| spellingShingle | Hannah K. Ruple Eva Haasis Anna Bettenburg Carina Maier Carolin Fritz Laura Schüle Sarah Löcker Yvonne Soltow Lynn Schintgen Nina S. Schmidt Celine Schneider Axel Lorentz W. Florian Fricke The gut microbiota predicts and time-restricted feeding delays experimental colitis Gut Microbes Biological clock gut microbiota circadian rhythm time-restricted feeding experimental colitis IL-10 |
| title | The gut microbiota predicts and time-restricted feeding delays experimental colitis |
| title_full | The gut microbiota predicts and time-restricted feeding delays experimental colitis |
| title_fullStr | The gut microbiota predicts and time-restricted feeding delays experimental colitis |
| title_full_unstemmed | The gut microbiota predicts and time-restricted feeding delays experimental colitis |
| title_short | The gut microbiota predicts and time-restricted feeding delays experimental colitis |
| title_sort | gut microbiota predicts and time restricted feeding delays experimental colitis |
| topic | Biological clock gut microbiota circadian rhythm time-restricted feeding experimental colitis IL-10 |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2025.2453019 |
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