Methylene blue at recommended concentrations alters metabolism in early zebrafish development

Abstract Methylene blue (MB) is an antifungal agent widely used during critical stages of zebrafish development. Most guidelines recommend 0.00005% or 0.0001% of MB for embryo/larval rearing. The Organisation for Economic Co-operation and Development zebrafish embryo toxicity test guideline omits MB...

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Bibliographic Details
Main Authors: Niepukolie Nipu, Lai Wei, Lauren Hamilton, Hyojin Lee, Jith Thomas, Jan A. Mennigen
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-07471-8
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