Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits
Incurable neuroretinal degeneration diseases cause severe vision loss and blindness in millions of patients worldwide. In previous studies, we demonstrated that transplanting human bone marrow stromal cells (hBMSCs) in the extravascular spaces of the choroid (EVSC) of the Royal College of Surgeon ra...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/4061975 |
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| author | Adi Tzameret Sapir E. Kalish Ifat Sher Lea Twito Amilia Meir Itay Levy Shlomo Margel Iris Moroz Mordechai Rosner Avraham J. Treves Arnon Nagler Michael Belkin Ygal Rotenstreich |
| author_facet | Adi Tzameret Sapir E. Kalish Ifat Sher Lea Twito Amilia Meir Itay Levy Shlomo Margel Iris Moroz Mordechai Rosner Avraham J. Treves Arnon Nagler Michael Belkin Ygal Rotenstreich |
| author_sort | Adi Tzameret |
| collection | DOAJ |
| description | Incurable neuroretinal degeneration diseases cause severe vision loss and blindness in millions of patients worldwide. In previous studies, we demonstrated that transplanting human bone marrow stromal cells (hBMSCs) in the extravascular spaces of the choroid (EVSC) of the Royal College of Surgeon rats ameliorated retinal degeneration for up to 5 months. Assessing the safety of hBMSC treatment and graft survival in a large animal is a crucial step before initiating clinical trials. Here, we transplanted hBMSCs into the EVSC compartment of New Zealand White rabbits. No immunosuppressants were used. Transplanted cells were spread across the EVSC covering over 80 percent of the subretinal surface. No cells were detected in the sclera. Cells were retained in the EVSC compartment 10 weeks following transplantation. Spectral domain optical coherence tomography (SD-OCT) and histopathology analysis demonstrated no choroidal hemorrhages, retinal detachment, inflammation, or any untoward pathological reactions in any of transplanted eyes or in the control noninjected contralateral eyes. No reduction in retinal function was recorded by electroretinogram up to 10 weeks following transplantation. This study demonstrates the feasibility and safety of transplanting hBMSCs in the EVSC compartment in a large eye model of rabbits. |
| format | Article |
| id | doaj-art-c7764a28034a47b98e6070922ddee56c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c7764a28034a47b98e6070922ddee56c2025-02-03T01:24:29ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/40619754061975Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of RabbitsAdi Tzameret0Sapir E. Kalish1Ifat Sher2Lea Twito3Amilia Meir4Itay Levy5Shlomo Margel6Iris Moroz7Mordechai Rosner8Avraham J. Treves9Arnon Nagler10Michael Belkin11Ygal Rotenstreich12Maurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelCenter for Stem Cells and Regenerative Medicine, Sheba Medical Center, Tel Hashomer, IsraelDepartment of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat Gan 52900, IsraelDepartment of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat Gan 52900, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelCenter for Stem Cells and Regenerative Medicine, Sheba Medical Center, Tel Hashomer, IsraelHematology Division, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelMaurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, IsraelIncurable neuroretinal degeneration diseases cause severe vision loss and blindness in millions of patients worldwide. In previous studies, we demonstrated that transplanting human bone marrow stromal cells (hBMSCs) in the extravascular spaces of the choroid (EVSC) of the Royal College of Surgeon rats ameliorated retinal degeneration for up to 5 months. Assessing the safety of hBMSC treatment and graft survival in a large animal is a crucial step before initiating clinical trials. Here, we transplanted hBMSCs into the EVSC compartment of New Zealand White rabbits. No immunosuppressants were used. Transplanted cells were spread across the EVSC covering over 80 percent of the subretinal surface. No cells were detected in the sclera. Cells were retained in the EVSC compartment 10 weeks following transplantation. Spectral domain optical coherence tomography (SD-OCT) and histopathology analysis demonstrated no choroidal hemorrhages, retinal detachment, inflammation, or any untoward pathological reactions in any of transplanted eyes or in the control noninjected contralateral eyes. No reduction in retinal function was recorded by electroretinogram up to 10 weeks following transplantation. This study demonstrates the feasibility and safety of transplanting hBMSCs in the EVSC compartment in a large eye model of rabbits.http://dx.doi.org/10.1155/2017/4061975 |
| spellingShingle | Adi Tzameret Sapir E. Kalish Ifat Sher Lea Twito Amilia Meir Itay Levy Shlomo Margel Iris Moroz Mordechai Rosner Avraham J. Treves Arnon Nagler Michael Belkin Ygal Rotenstreich Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits Stem Cells International |
| title | Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits |
| title_full | Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits |
| title_fullStr | Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits |
| title_full_unstemmed | Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits |
| title_short | Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits |
| title_sort | long term safety of transplanting human bone marrow stromal cells into the extravascular spaces of the choroid of rabbits |
| url | http://dx.doi.org/10.1155/2017/4061975 |
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