Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the enviro...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/489569 |
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| author | Eulalia de la Torre Rocío Colello Nora Lía Padola Analía Etcheverría Edgardo Rodríguez Fabián Amanto María Ofelia Tapia Alejandro Luis Soraci |
| author_facet | Eulalia de la Torre Rocío Colello Nora Lía Padola Analía Etcheverría Edgardo Rodríguez Fabián Amanto María Ofelia Tapia Alejandro Luis Soraci |
| author_sort | Eulalia de la Torre |
| collection | DOAJ |
| description | Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health. |
| format | Article |
| id | doaj-art-c752cbfd7bb74135a24036fe9fa42331 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-c752cbfd7bb74135a24036fe9fa423312025-02-03T01:32:22ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/489569489569Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production SystemEulalia de la Torre0Rocío Colello1Nora Lía Padola2Analía Etcheverría3Edgardo Rodríguez4Fabián Amanto5María Ofelia Tapia6Alejandro Luis Soraci7Área de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Bioestadística, Departamento de SAMP, CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Producción Porcina, Departamento de Producción Animal, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaÁrea de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, ArgentinaIntegrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.http://dx.doi.org/10.1155/2014/489569 |
| spellingShingle | Eulalia de la Torre Rocío Colello Nora Lía Padola Analía Etcheverría Edgardo Rodríguez Fabián Amanto María Ofelia Tapia Alejandro Luis Soraci Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System International Journal of Microbiology |
| title | Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System |
| title_full | Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System |
| title_fullStr | Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System |
| title_full_unstemmed | Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System |
| title_short | Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System |
| title_sort | detection of integrase gene in e coli isolated from pigs at different stages of production system |
| url | http://dx.doi.org/10.1155/2014/489569 |
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