Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019
Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The study is hospital-based surveillance of rotavirus diarrhea in children from Imphal, Manipur, India conducted from December 2015 to March 2019. The positivity rate was found to be high ∼69.25% (358/517)...
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Elsevier
2025-03-01
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| author | Damayanti Yengkhom Devi Chongtham Shyamsunder Singh C. Durga Rao Nima D. Namsa |
| author_facet | Damayanti Yengkhom Devi Chongtham Shyamsunder Singh C. Durga Rao Nima D. Namsa |
| author_sort | Damayanti Yengkhom Devi |
| collection | DOAJ |
| description | Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The study is hospital-based surveillance of rotavirus diarrhea in children from Imphal, Manipur, India conducted from December 2015 to March 2019. The positivity rate was found to be high ∼69.25% (358/517) and proportion of diarrhea cases and rotavirus diarrhea was peak in winter months and mostly in children from 6 to 24 months. G3 (43%) was the most widely circulating genotype in Imphal followed by G1 (16%), G2 (8%), G9 (5%), G8 (3%), G10 (1%), and G4 (1%), while G12 (0.26%) was rarely detected. Among P-types, P[6] (22%) accounted for the highest prevalence followed by P[8] (11%) and P[4] (4%), P[11] (4%), P[10] (3%), P-type mixed infection 3%, while 53% were untypeable. In G/P combinations, we detected 22 different rotavirus strains at varying frequencies. Globally distributed G3P[8] and G1P[8] strains were observed in the study. G3P[6] emerged as the most predominant rotavirus strain followed by G3P[8], G1P[6], G1P[8], and G9P[6]. The common rotavirus strains distributed across the region namely G3P[8], G1P[8], G2P[4], G9P[4], G1P[4], G1P[6], and G9P[6] were also observed. Interestingly, our study has observed a high percentage of unusual strains namely G9P[4], G1P[11], G2P[11], G3P[10], G3P[11], G4P[11], G9P[10], G9P[11],G10P[6], and G10P[8]. Of note, the high frequency of non-typeable rotavirus P-types (56%) are suggestive of point mutations that might have accumulated in the primer-binding region of VP4 gene. The findings of the present study revealed the hospital-based prevalence of rotavirus disease and the circulating genotypes during the pre-vaccination period and highlights the need for continuous surveillance of rotavirus infection post-rotavac vaccine introduction in the state of Manipur, India. |
| format | Article |
| id | doaj-art-c74bbdda9b824c82a6dc6e96fca9e051 |
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| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-c74bbdda9b824c82a6dc6e96fca9e0512025-08-20T02:18:47ZengElsevierVaccine: X2590-13622025-03-012310061410.1016/j.jvacx.2025.100614Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019Damayanti Yengkhom Devi0Chongtham Shyamsunder Singh1C. Durga Rao2Nima D. Namsa3Department of Molecular Biology and Biotechnology, Tezpur University, Napaam 784 028, Assam, IndiaDepartment of Paediatrics, Regional Institute of Medical Sciences, Lamphelpat, Imphal, Manipur 795004, IndiaDepartment of Biological Sciences, SRM University AP, Amaravati, Mangalagiri, Andhra Pradesh 522240, IndiaDepartment of Molecular Biology and Biotechnology, Tezpur University, Napaam 784 028, Assam, India; Corresponding author.Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The study is hospital-based surveillance of rotavirus diarrhea in children from Imphal, Manipur, India conducted from December 2015 to March 2019. The positivity rate was found to be high ∼69.25% (358/517) and proportion of diarrhea cases and rotavirus diarrhea was peak in winter months and mostly in children from 6 to 24 months. G3 (43%) was the most widely circulating genotype in Imphal followed by G1 (16%), G2 (8%), G9 (5%), G8 (3%), G10 (1%), and G4 (1%), while G12 (0.26%) was rarely detected. Among P-types, P[6] (22%) accounted for the highest prevalence followed by P[8] (11%) and P[4] (4%), P[11] (4%), P[10] (3%), P-type mixed infection 3%, while 53% were untypeable. In G/P combinations, we detected 22 different rotavirus strains at varying frequencies. Globally distributed G3P[8] and G1P[8] strains were observed in the study. G3P[6] emerged as the most predominant rotavirus strain followed by G3P[8], G1P[6], G1P[8], and G9P[6]. The common rotavirus strains distributed across the region namely G3P[8], G1P[8], G2P[4], G9P[4], G1P[4], G1P[6], and G9P[6] were also observed. Interestingly, our study has observed a high percentage of unusual strains namely G9P[4], G1P[11], G2P[11], G3P[10], G3P[11], G4P[11], G9P[10], G9P[11],G10P[6], and G10P[8]. Of note, the high frequency of non-typeable rotavirus P-types (56%) are suggestive of point mutations that might have accumulated in the primer-binding region of VP4 gene. The findings of the present study revealed the hospital-based prevalence of rotavirus disease and the circulating genotypes during the pre-vaccination period and highlights the need for continuous surveillance of rotavirus infection post-rotavac vaccine introduction in the state of Manipur, India.http://www.sciencedirect.com/science/article/pii/S2590136225000087RotavirusAcute gastroenteritis (AG)EpidemiologySurveillanceGenotypeElectropherotyping |
| spellingShingle | Damayanti Yengkhom Devi Chongtham Shyamsunder Singh C. Durga Rao Nima D. Namsa Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 Vaccine: X Rotavirus Acute gastroenteritis (AG) Epidemiology Surveillance Genotype Electropherotyping |
| title | Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 |
| title_full | Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 |
| title_fullStr | Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 |
| title_full_unstemmed | Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 |
| title_short | Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019 |
| title_sort | genetic diversity and prevalence of group a rotavirus infection in children of imphal manipur india a hospital based surveillance study conducted during december 2015 to march 2019 |
| topic | Rotavirus Acute gastroenteritis (AG) Epidemiology Surveillance Genotype Electropherotyping |
| url | http://www.sciencedirect.com/science/article/pii/S2590136225000087 |
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