MOMAST<sup>®</sup> Downregulates AQP3 Expression and Function in Human Colon Cells
The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role in water transport across human colonic surface cells. Beyond water, AQP3 can mediate glycerol and H<sub>2</sub>O<sub>2</sub> transport. Abnormal expression and function of...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/1/26 |
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| Summary: | The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role in water transport across human colonic surface cells. Beyond water, AQP3 can mediate glycerol and H<sub>2</sub>O<sub>2</sub> transport. Abnormal expression and function of AQP3 have been found in various diseases often characterized by altered cell growth and proliferation. Here, the beneficial effects of MOMAST<sup>®</sup> have been evaluated. MOMAST<sup>®</sup> is an antioxidant-patented natural phenolic complex obtained from olive wastewater (OWW) of the Coratina cultivar. Treatment of human colon HCT8 cells with MOMAST<sup>®</sup> reduced cell viability. Confocal studies and Western Blotting analysis demonstrated that treatment with MOMAST<sup>®</sup> significantly decreased the staining and the expression of AQP3. Importantly, functional studies revealed that the reduction of AQP3 abundance correlates with a significant decrease in glycerol and H<sub>2</sub>O<sub>2</sub> uptake. Indeed, the H<sub>2</sub>O<sub>2</sub> transport was partially but significantly reduced in the presence of MOMAST<sup>®</sup> or DFP00173, a selective inhibitor of AQP3. In addition, the MOMAST<sup>®</sup>-induced AQP3 decrease was associated with reduced epithelial-mesenchymal transition (EMT)-related proteins such as vimentin and β-catenin. Together, these findings propose MOMAST<sup>®</sup> as a potential adjuvant in colon diseases associated with abnormal cell growth by targeting AQP3. |
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| ISSN: | 2076-3921 |