Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study
Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S phase of the cell cycle, and immunohistochemical detection of the protein represents a useful marker for the proliferating fraction of cells in tissue specimens. PCNA expression was studied in odontogenic...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/105193 |
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| author | Hiroshi Takahashi Shuichi Fujita Shigeru Yamabe Takeshi Moriishi Haruo Okabe Yoshifumi Tajima Akio Mizuno |
| author_facet | Hiroshi Takahashi Shuichi Fujita Shigeru Yamabe Takeshi Moriishi Haruo Okabe Yoshifumi Tajima Akio Mizuno |
| author_sort | Hiroshi Takahashi |
| collection | DOAJ |
| description | Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S phase of the cell cycle, and immunohistochemical detection of the protein represents a useful marker for the proliferating fraction of cells in tissue specimens. PCNA expression was studied in odontogenic keratocysts (n = 15) and ameloblastomas (n = 46) using an avidin–biotin–peroxidase complex method on routinely processed paraffin sections. The percentage of PCNA-positive cells determined by point counting was significantly lower in the ameloblastomas (mean 9.4%, standard deviation (SD) 11.0) than in odontogenic keratocysts (mean 29.9%, SD 24.0). In ameloblastomas, the mean percentage of PCNA-positive cells was lowest in the acanthomatous pattern and highest in plexiform pattern. The mean percentage of PCNA-positive cells in plexiform pattern was non-significantly higher than that in follicular pattern. The mean percentage of PCNA-positive cells in plexiform and follicular patterns was significantly higher than that in cyctic and acanthomatous patterns. The frequency of PCNA-positive cells was significantly higher in the peripheral cells of follicular and plexiform patterns than in the central cells of both patterns (p < 0.01). Therefore, peripheral cells were regarded as reserve cell of central cells. The mean percentage of PCNA-positive cells in the epithelial lining of odontogenic keratocyst was not significantly different from those in the peripheral cells of follicular and plexiform patterns of ameloblastoma. In contrast, the odontogenic keratocyst exhibited a mean percentage of PCNA-positive cells which was statistically higher than that in other histological elements of ameloblastomas. The present study suggests that odontogenic keratocyst is regarded as benign odontogenic tumour. |
| format | Article |
| id | doaj-art-c6cf24a3dac0478d8e31e4cf17f3c623 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-c6cf24a3dac0478d8e31e4cf17f3c6232025-02-03T05:46:28ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0116418519210.1155/1998/105193Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical StudyHiroshi Takahashi0Shuichi Fujita1Shigeru Yamabe2Takeshi Moriishi3Haruo Okabe4Yoshifumi Tajima5Akio Mizuno6Department of Oral Pathology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8588, JapanDepartment of Oral Pathology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8588, JapanFirst Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Nagasaki, JapanDepartment of Oral Pathology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8588, JapanDepartment of Oral Pathology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8588, JapanDepartment of Oral Pathology, Meikai University School of Dentistry, Sakado, Saitama, JapanFirst Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Nagasaki, JapanProliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S phase of the cell cycle, and immunohistochemical detection of the protein represents a useful marker for the proliferating fraction of cells in tissue specimens. PCNA expression was studied in odontogenic keratocysts (n = 15) and ameloblastomas (n = 46) using an avidin–biotin–peroxidase complex method on routinely processed paraffin sections. The percentage of PCNA-positive cells determined by point counting was significantly lower in the ameloblastomas (mean 9.4%, standard deviation (SD) 11.0) than in odontogenic keratocysts (mean 29.9%, SD 24.0). In ameloblastomas, the mean percentage of PCNA-positive cells was lowest in the acanthomatous pattern and highest in plexiform pattern. The mean percentage of PCNA-positive cells in plexiform pattern was non-significantly higher than that in follicular pattern. The mean percentage of PCNA-positive cells in plexiform and follicular patterns was significantly higher than that in cyctic and acanthomatous patterns. The frequency of PCNA-positive cells was significantly higher in the peripheral cells of follicular and plexiform patterns than in the central cells of both patterns (p < 0.01). Therefore, peripheral cells were regarded as reserve cell of central cells. The mean percentage of PCNA-positive cells in the epithelial lining of odontogenic keratocyst was not significantly different from those in the peripheral cells of follicular and plexiform patterns of ameloblastoma. In contrast, the odontogenic keratocyst exhibited a mean percentage of PCNA-positive cells which was statistically higher than that in other histological elements of ameloblastomas. The present study suggests that odontogenic keratocyst is regarded as benign odontogenic tumour.http://dx.doi.org/10.1155/1998/105193 |
| spellingShingle | Hiroshi Takahashi Shuichi Fujita Shigeru Yamabe Takeshi Moriishi Haruo Okabe Yoshifumi Tajima Akio Mizuno Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study Analytical Cellular Pathology |
| title | Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study |
| title_full | Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study |
| title_fullStr | Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study |
| title_full_unstemmed | Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study |
| title_short | Comparison of Proliferating Cell Nuclear Antigen Expression in Odontogenic Keratocyst and Ameloblastoma: An Immunohistochemical Study |
| title_sort | comparison of proliferating cell nuclear antigen expression in odontogenic keratocyst and ameloblastoma an immunohistochemical study |
| url | http://dx.doi.org/10.1155/1998/105193 |
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