Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia
In this study, for the first time, the volatile fraction from two domesticated <i>Capsicum annuum</i> accessions (“Paprika” and “Baklouti”) collected in Tunisia was investigated by two complementary analytical techniques, such as Solid-Phase Microextraction–Gas Chromatography/Mass Spectr...
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2024-11-01
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| author | Annalisa Serio Francesca Maggio Anis Ben Hsouna Rania Ben Saad Cosimo Taiti Stefania Garzoli |
| author_facet | Annalisa Serio Francesca Maggio Anis Ben Hsouna Rania Ben Saad Cosimo Taiti Stefania Garzoli |
| author_sort | Annalisa Serio |
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| description | In this study, for the first time, the volatile fraction from two domesticated <i>Capsicum annuum</i> accessions (“Paprika” and “Baklouti”) collected in Tunisia was investigated by two complementary analytical techniques, such as Solid-Phase Microextraction–Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction–Time-of-Flight–Mass Spectrometry (PTR-ToF-MS). The obtained results highlighted the presence of a high number of Volatile Organic Compounds (VOCs), including monoterpene and sesquiterpene compounds with <i>α</i>-curcumene, I-zingiberene, <i>β</i>-bisabolene and <i>β</i>-sesquiphellandrene as the major components. In addition, GC/MS was used to investigate the non-volatile chemical composition of the dried powders and their extracts, which were found to be rich in sulfur compounds, fatty acids and sugars. Eleven bacterial strains were chosen to assess the antimicrobial effectiveness of the extracts. The results showed that the extracts exhibited strain-dependent behavior, and the type strains displayed a greater susceptibility to the treatments, if compared to the wild strains, and, in particular, showed the best antimicrobial activity against Gram-positive bacteria, such as <i>Listeria monocytogenes</i> and <i>Staphylococcus aureus.</i> |
| format | Article |
| id | doaj-art-c696c1a4348643ee94e3eae723dd9a06 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-c696c1a4348643ee94e3eae723dd9a062025-08-20T02:48:01ZengMDPI AGMolecules1420-30492024-11-012922523610.3390/molecules29225236Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from TunisiaAnnalisa Serio0Francesca Maggio1Anis Ben Hsouna2Rania Ben Saad3Cosimo Taiti4Stefania Garzoli5Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyDepartment of Environmental Sciences and Nutrition, Higher Institute of Applied Sciences and Technology of Mahdia, University of Monastir, Monastir 5000, TunisiaLaboratory of Biotechnology and Plant Improvement, Centre of Biotechnology of Sfax, University of Sfax, Sfax 3018, TunisiaDepartment of Agri-Food and Environmental Science, Università di Firenze, 50019 Firenze, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Rome, ItalyIn this study, for the first time, the volatile fraction from two domesticated <i>Capsicum annuum</i> accessions (“Paprika” and “Baklouti”) collected in Tunisia was investigated by two complementary analytical techniques, such as Solid-Phase Microextraction–Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction–Time-of-Flight–Mass Spectrometry (PTR-ToF-MS). The obtained results highlighted the presence of a high number of Volatile Organic Compounds (VOCs), including monoterpene and sesquiterpene compounds with <i>α</i>-curcumene, I-zingiberene, <i>β</i>-bisabolene and <i>β</i>-sesquiphellandrene as the major components. In addition, GC/MS was used to investigate the non-volatile chemical composition of the dried powders and their extracts, which were found to be rich in sulfur compounds, fatty acids and sugars. Eleven bacterial strains were chosen to assess the antimicrobial effectiveness of the extracts. The results showed that the extracts exhibited strain-dependent behavior, and the type strains displayed a greater susceptibility to the treatments, if compared to the wild strains, and, in particular, showed the best antimicrobial activity against Gram-positive bacteria, such as <i>Listeria monocytogenes</i> and <i>Staphylococcus aureus.</i>https://www.mdpi.com/1420-3049/29/22/5236HS-SPME-GC/MS analysisPTR-ToF-MS analysissecondary metabolitesantimicrobial activity |
| spellingShingle | Annalisa Serio Francesca Maggio Anis Ben Hsouna Rania Ben Saad Cosimo Taiti Stefania Garzoli Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia Molecules HS-SPME-GC/MS analysis PTR-ToF-MS analysis secondary metabolites antimicrobial activity |
| title | Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia |
| title_full | Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia |
| title_fullStr | Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia |
| title_full_unstemmed | Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia |
| title_short | Exploring the Metabolome and Antimicrobial Properties of <i>Capsicum annuum</i> L. (Baklouti and Paprika) Dried Powders from Tunisia |
| title_sort | exploring the metabolome and antimicrobial properties of i capsicum annuum i l baklouti and paprika dried powders from tunisia |
| topic | HS-SPME-GC/MS analysis PTR-ToF-MS analysis secondary metabolites antimicrobial activity |
| url | https://www.mdpi.com/1420-3049/29/22/5236 |
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