TCTEX1D2 is essential for sperm flagellum formation in mice
Abstract Flagella and cilia are widely conserved motile structures, in mammalian, sperm possess flagella. Large protein complexes called dynein, including cytoplasmic dynein 2 and axonemal dynein, play a role in the formation of cilia and flagella. The function of each subunit component of dynein co...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-83424-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594792265023488 |
|---|---|
| author | Ryua Harima Kenshiro Hara Kentaro Tanemura |
| author_facet | Ryua Harima Kenshiro Hara Kentaro Tanemura |
| author_sort | Ryua Harima |
| collection | DOAJ |
| description | Abstract Flagella and cilia are widely conserved motile structures, in mammalian, sperm possess flagella. Large protein complexes called dynein, including cytoplasmic dynein 2 and axonemal dynein, play a role in the formation of cilia and flagella. The function of each subunit component of dynein complexes in sperm flagellum formation remains unclear. One such subunit is TCTEX1D2. Co-immunoprecipitation studies showed that TCTEX1D2 interacted with cytoplasmic dynein 2 subunits WDR34, WDR60, and DYNLT1 in the testes. Furthermore, TCTEX1D2 also interacted with WDR63 and WDR78, subunits of inner dynein arm, which is axonemal dynein. Tctex1d2 −/− mice generated in this study exhibited male infertility due to flagellar dysplasia, and the axonemal structures were disrupted inside the flagella. Further, the localization of cytoplasmic dynein 2 subunits was abnormal in in Tctex1d2 −/− mice. In contrast, the motile cilia of Tctex1d2 −/− mice were normal. Overall, we revealed that TCTEX1D2 is important for the assembly of cytoplasmic dynein 2 and inner dynein arm and functions in two distinct dynein complexes during mouse sperm flagellum formation. This is only in sperm flagellum formation, not in cilia formation. |
| format | Article |
| id | doaj-art-c685781fbaeb427cb80bcb5e470bc68b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c685781fbaeb427cb80bcb5e470bc68b2025-01-19T12:19:37ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-024-83424-1TCTEX1D2 is essential for sperm flagellum formation in miceRyua Harima0Kenshiro Hara1Kentaro Tanemura2Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku UniversityAbstract Flagella and cilia are widely conserved motile structures, in mammalian, sperm possess flagella. Large protein complexes called dynein, including cytoplasmic dynein 2 and axonemal dynein, play a role in the formation of cilia and flagella. The function of each subunit component of dynein complexes in sperm flagellum formation remains unclear. One such subunit is TCTEX1D2. Co-immunoprecipitation studies showed that TCTEX1D2 interacted with cytoplasmic dynein 2 subunits WDR34, WDR60, and DYNLT1 in the testes. Furthermore, TCTEX1D2 also interacted with WDR63 and WDR78, subunits of inner dynein arm, which is axonemal dynein. Tctex1d2 −/− mice generated in this study exhibited male infertility due to flagellar dysplasia, and the axonemal structures were disrupted inside the flagella. Further, the localization of cytoplasmic dynein 2 subunits was abnormal in in Tctex1d2 −/− mice. In contrast, the motile cilia of Tctex1d2 −/− mice were normal. Overall, we revealed that TCTEX1D2 is important for the assembly of cytoplasmic dynein 2 and inner dynein arm and functions in two distinct dynein complexes during mouse sperm flagellum formation. This is only in sperm flagellum formation, not in cilia formation.https://doi.org/10.1038/s41598-024-83424-1TCTEX1D2SpermatogenesisSperm flagellaCytoplasmic dynein 2Inner dynein armMale infertility |
| spellingShingle | Ryua Harima Kenshiro Hara Kentaro Tanemura TCTEX1D2 is essential for sperm flagellum formation in mice Scientific Reports TCTEX1D2 Spermatogenesis Sperm flagella Cytoplasmic dynein 2 Inner dynein arm Male infertility |
| title | TCTEX1D2 is essential for sperm flagellum formation in mice |
| title_full | TCTEX1D2 is essential for sperm flagellum formation in mice |
| title_fullStr | TCTEX1D2 is essential for sperm flagellum formation in mice |
| title_full_unstemmed | TCTEX1D2 is essential for sperm flagellum formation in mice |
| title_short | TCTEX1D2 is essential for sperm flagellum formation in mice |
| title_sort | tctex1d2 is essential for sperm flagellum formation in mice |
| topic | TCTEX1D2 Spermatogenesis Sperm flagella Cytoplasmic dynein 2 Inner dynein arm Male infertility |
| url | https://doi.org/10.1038/s41598-024-83424-1 |
| work_keys_str_mv | AT ryuaharima tctex1d2isessentialforspermflagellumformationinmice AT kenshirohara tctex1d2isessentialforspermflagellumformationinmice AT kentarotanemura tctex1d2isessentialforspermflagellumformationinmice |