Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.)
IntroductionFusarium crown rot (FCR) is a severe soil-borne disease that affects wheat globally and leads to significant yield reductions. Identifying the loci associated with resistance to FCR and developing corresponding markers are essential for the breeding of resistant wheat varieties.MethodsIn...
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Frontiers Media S.A.
2024-11-01
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| author | Faji Li Can Guo Qi Zhao Weie Wen Shengnan Zhai Xinyou Cao Cheng Liu Dungong Cheng Jun Guo Yan Zi Aifeng Liu Jianmin Song Jianjun Liu Jindong Liu Haosheng Li |
| author_facet | Faji Li Can Guo Qi Zhao Weie Wen Shengnan Zhai Xinyou Cao Cheng Liu Dungong Cheng Jun Guo Yan Zi Aifeng Liu Jianmin Song Jianjun Liu Jindong Liu Haosheng Li |
| author_sort | Faji Li |
| collection | DOAJ |
| description | IntroductionFusarium crown rot (FCR) is a severe soil-borne disease that affects wheat globally and leads to significant yield reductions. Identifying the loci associated with resistance to FCR and developing corresponding markers are essential for the breeding of resistant wheat varieties.MethodsIn this study, we evaluated the resistance to FCR in a recombinant inbred line (RIL) population originating from Gaocheng 8901 and Zhoumai 16 across four environments. The RILs and their parents were genotyped using a wheat 90K singlenucleotide polymorphism (SNP) array.ResultsWe identified a total of five quantitative trait loci (QTLs) related to FCR resistance: QFCR.caas-3AL, QFCR.caas-3DL, QFCR.caas-5BL, QFCR.caas-6BS, and QFCR.caas-7DS. These QTLs accounted for 4.6% to 12.8% of the phenotypic variance. Notably, QFCR.caas-5BL and QFCR.caas-6BS had been previously detected, whereas QFCR.caas-3AL, QFCR.caas-3DL, and QFCR.caas-7DS are novel loci. The favorable alleles of QFCR.caas-3DL and QFCR.caas-5BL were contributed by Zhoumai 16, while the favorable alleles for QFCR.caas-3AL, QFCR.caas-6BS, and QFCR.caas-7DS originated from Gaocheng 8901. Additionally, this study identified seven candidate genes that encode disease resistance proteins, the BTB/POZ domains, peroxidase activity, and leucine-rich repeat receptor-like protein kinase. Furthermore, we developed and validated two kompetitive allele-specific PCR (KASP) markers, Kasp_3AL_FCR (QFCR.caas-3AL) and Kasp_5BL_FCR (QFCR.caas-5BL), in a natural population of 202 wheat varieties.DiscussionThis study contributes new genetic insights and provides new stable loci and available KASP markers for breeding to enhance FCR resistance in common wheat. |
| format | Article |
| id | doaj-art-c67b94c9ceca4fc18b9bcd9222a36759 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-c67b94c9ceca4fc18b9bcd9222a367592025-02-05T14:12:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14574371457437Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.)Faji Li0Can Guo1Qi Zhao2Weie Wen3Shengnan Zhai4Xinyou Cao5Cheng Liu6Dungong Cheng7Jun Guo8Yan Zi9Aifeng Liu10Jianmin Song11Jianjun Liu12Jindong Liu13Haosheng Li14Crop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaShangqiu Academy of Agriculture and Forestry Sciences, Shangqiu, ChinaCollage of Life Science, Yantai University, Yantai, ChinaDepartment of Cell Biology, Zunyi Medical University, Zunyi, Guizhou, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaCrop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, ChinaIntroductionFusarium crown rot (FCR) is a severe soil-borne disease that affects wheat globally and leads to significant yield reductions. Identifying the loci associated with resistance to FCR and developing corresponding markers are essential for the breeding of resistant wheat varieties.MethodsIn this study, we evaluated the resistance to FCR in a recombinant inbred line (RIL) population originating from Gaocheng 8901 and Zhoumai 16 across four environments. The RILs and their parents were genotyped using a wheat 90K singlenucleotide polymorphism (SNP) array.ResultsWe identified a total of five quantitative trait loci (QTLs) related to FCR resistance: QFCR.caas-3AL, QFCR.caas-3DL, QFCR.caas-5BL, QFCR.caas-6BS, and QFCR.caas-7DS. These QTLs accounted for 4.6% to 12.8% of the phenotypic variance. Notably, QFCR.caas-5BL and QFCR.caas-6BS had been previously detected, whereas QFCR.caas-3AL, QFCR.caas-3DL, and QFCR.caas-7DS are novel loci. The favorable alleles of QFCR.caas-3DL and QFCR.caas-5BL were contributed by Zhoumai 16, while the favorable alleles for QFCR.caas-3AL, QFCR.caas-6BS, and QFCR.caas-7DS originated from Gaocheng 8901. Additionally, this study identified seven candidate genes that encode disease resistance proteins, the BTB/POZ domains, peroxidase activity, and leucine-rich repeat receptor-like protein kinase. Furthermore, we developed and validated two kompetitive allele-specific PCR (KASP) markers, Kasp_3AL_FCR (QFCR.caas-3AL) and Kasp_5BL_FCR (QFCR.caas-5BL), in a natural population of 202 wheat varieties.DiscussionThis study contributes new genetic insights and provides new stable loci and available KASP markers for breeding to enhance FCR resistance in common wheat.https://www.frontiersin.org/articles/10.3389/fpls.2024.1457437/fullcommon wheatFusarium crown rot (FCR)molecular marker-assisted selectionquantitative trait Loci (QTL)kompetitive allele-specific PCR (KASP) |
| spellingShingle | Faji Li Can Guo Qi Zhao Weie Wen Shengnan Zhai Xinyou Cao Cheng Liu Dungong Cheng Jun Guo Yan Zi Aifeng Liu Jianmin Song Jianjun Liu Jindong Liu Haosheng Li Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) Frontiers in Plant Science common wheat Fusarium crown rot (FCR) molecular marker-assisted selection quantitative trait Loci (QTL) kompetitive allele-specific PCR (KASP) |
| title | Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) |
| title_full | Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) |
| title_fullStr | Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) |
| title_short | Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.) |
| title_sort | genome wide linkage mapping of fusarium crown rot in common wheat triticum aestivum l |
| topic | common wheat Fusarium crown rot (FCR) molecular marker-assisted selection quantitative trait Loci (QTL) kompetitive allele-specific PCR (KASP) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1457437/full |
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