Genomic adaptation of Clostridium perfringens to human intestine
Abstract Clostridium perfringens is often associated with foodborne diseases, posing significant public health risks. However, genomic investigation of C. perfringens isolates from the human population has been lacking. This study aims to fill this knowledge gap by examining the genomic characterist...
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Wiley
2024-12-01
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| Series: | iMetaOmics |
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| Online Access: | https://doi.org/10.1002/imo2.38 |
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| author | Ke Wu Juan Wang Zelin Yan Yanyan Zhu Shaolin Wang Bo Fu Chengtao Sun Ruichao Li Edward M. Fox Séamus Fanning Li Bai Yang Wang Yizhi Tang Zhe Yin Rong Zhang Hongning Wang |
| author_facet | Ke Wu Juan Wang Zelin Yan Yanyan Zhu Shaolin Wang Bo Fu Chengtao Sun Ruichao Li Edward M. Fox Séamus Fanning Li Bai Yang Wang Yizhi Tang Zhe Yin Rong Zhang Hongning Wang |
| author_sort | Ke Wu |
| collection | DOAJ |
| description | Abstract Clostridium perfringens is often associated with foodborne diseases, posing significant public health risks. However, genomic investigation of C. perfringens isolates from the human population has been lacking. This study aims to fill this knowledge gap by examining the genomic characteristics of C. perfringens isolates from 699 individuals at a provincial hospital in China. We further conducted evolutionary and pan‐genomic analyses, incorporating isolates from humans and animals worldwide. The results reveal potential regional and transregional transmission of C. perfringens among individuals, along with the common transfer of small gene clusters during this process. Notably, the food poisoning‐associated toxin gene cpe was identified in a fusion plasmid for the first time in an isolate, indicating fusion of pCP13‐like and pCW3‐like plasmids and the potential for transfer of cpe across genetic backgrounds. Moreover, we observed that the genomic characteristics of C. perfringens correlate with host species, with specific toxin genes, such as pfoA and colA, potentially influencing host selectivity. Through this comprehensive genomic analysis, we provide novel insights into the fusion of pCW3‐like and pCP13‐like plasmids, the genetic location of cpe, the transmission dynamics of C. perfringens strains, and the relationship between toxin genes and host relevance. These findings expand our understanding of C. perfringens and its implications for public health. |
| format | Article |
| id | doaj-art-c66b880991b24ba2a40a994307b14ffd |
| institution | Kabale University |
| issn | 2996-9506 2996-9514 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | iMetaOmics |
| spelling | doaj-art-c66b880991b24ba2a40a994307b14ffd2025-01-31T16:15:24ZengWileyiMetaOmics2996-95062996-95142024-12-0112n/an/a10.1002/imo2.38Genomic adaptation of Clostridium perfringens to human intestineKe Wu0Juan Wang1Zelin Yan2Yanyan Zhu3Shaolin Wang4Bo Fu5Chengtao Sun6Ruichao Li7Edward M. Fox8Séamus Fanning9Li Bai10Yang Wang11Yizhi Tang12Zhe Yin13Rong Zhang14Hongning Wang15Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio‐Resource and Eco‐Environment of Ministry of Education, College of Life Sciences, Sichuan University Chengdu ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine Northwest A&F University Yangling ChinaDepartment of Clinical Laboratory Second Affiliated Hospital of Zhejiang University, School of Medicine Hangzhou ChinaDepartment of Clinical Laboratory Second Affiliated Hospital of Zhejiang University, School of Medicine Hangzhou ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine China Agricultural University Beijing ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu ChinaDepartment of Applied Sciences Northumbria University Newcastle upon Tyne UKResearch Unit of Food Safety Chinese Academy of Medical Sciences, NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA) Beijing ChinaResearch Unit of Food Safety Chinese Academy of Medical Sciences, NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA) Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine China Agricultural University Beijing ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio‐Resource and Eco‐Environment of Ministry of Education, College of Life Sciences, Sichuan University Chengdu ChinaState Key Laboratory of Pathogen and Biosecurity Academy of Military Medical Science Beijing ChinaDepartment of Clinical Laboratory Second Affiliated Hospital of Zhejiang University, School of Medicine Hangzhou ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio‐Resource and Eco‐Environment of Ministry of Education, College of Life Sciences, Sichuan University Chengdu ChinaAbstract Clostridium perfringens is often associated with foodborne diseases, posing significant public health risks. However, genomic investigation of C. perfringens isolates from the human population has been lacking. This study aims to fill this knowledge gap by examining the genomic characteristics of C. perfringens isolates from 699 individuals at a provincial hospital in China. We further conducted evolutionary and pan‐genomic analyses, incorporating isolates from humans and animals worldwide. The results reveal potential regional and transregional transmission of C. perfringens among individuals, along with the common transfer of small gene clusters during this process. Notably, the food poisoning‐associated toxin gene cpe was identified in a fusion plasmid for the first time in an isolate, indicating fusion of pCP13‐like and pCW3‐like plasmids and the potential for transfer of cpe across genetic backgrounds. Moreover, we observed that the genomic characteristics of C. perfringens correlate with host species, with specific toxin genes, such as pfoA and colA, potentially influencing host selectivity. Through this comprehensive genomic analysis, we provide novel insights into the fusion of pCW3‐like and pCP13‐like plasmids, the genetic location of cpe, the transmission dynamics of C. perfringens strains, and the relationship between toxin genes and host relevance. These findings expand our understanding of C. perfringens and its implications for public health.https://doi.org/10.1002/imo2.38Clostridium perfringenshost relevancepopulation structurevirulence factor |
| spellingShingle | Ke Wu Juan Wang Zelin Yan Yanyan Zhu Shaolin Wang Bo Fu Chengtao Sun Ruichao Li Edward M. Fox Séamus Fanning Li Bai Yang Wang Yizhi Tang Zhe Yin Rong Zhang Hongning Wang Genomic adaptation of Clostridium perfringens to human intestine iMetaOmics Clostridium perfringens host relevance population structure virulence factor |
| title | Genomic adaptation of Clostridium perfringens to human intestine |
| title_full | Genomic adaptation of Clostridium perfringens to human intestine |
| title_fullStr | Genomic adaptation of Clostridium perfringens to human intestine |
| title_full_unstemmed | Genomic adaptation of Clostridium perfringens to human intestine |
| title_short | Genomic adaptation of Clostridium perfringens to human intestine |
| title_sort | genomic adaptation of clostridium perfringens to human intestine |
| topic | Clostridium perfringens host relevance population structure virulence factor |
| url | https://doi.org/10.1002/imo2.38 |
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