Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts
In the present investigation, we have described the green biosynthesis of silver nanoparticles (AgNPs) using ripened fruit aqueous extract of Nothapodytes nimmoniana (Graham) Mabb. as capping agent. The antioxidant, anticancer and antimicrobial activities of AgNPs were also studied. UV analysis reve...
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| Language: | English |
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Tsinghua University Press
2016-12-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016301409 |
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| author | G. Mahendran B.D. Ranjitha Kumari |
| author_facet | G. Mahendran B.D. Ranjitha Kumari |
| author_sort | G. Mahendran |
| collection | DOAJ |
| description | In the present investigation, we have described the green biosynthesis of silver nanoparticles (AgNPs) using ripened fruit aqueous extract of Nothapodytes nimmoniana (Graham) Mabb. as capping agent. The antioxidant, anticancer and antimicrobial activities of AgNPs were also studied. UV analysis revealed that AgNPs had a sharp peak at 416 nm. X-ray diffraction (XRD) result confirmed the characteristic peaks indicated at 1 1 1, 2 0 0, 2 2 0 and 3 1 1 for the crystalline of the face centered cubic silver. The Scanning Electron Microscopy analysis results confirmed the spherical shaped of AgNPs with difference sizes of the particles and an average from 44 to 64 nm. Further, fruit extract and AgNPs were evaluated total phenolic, tannin and flavonoid contents and were subjected to assess their antioxidant potential using various in vitro systems such as using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), metal chelating, phosphomolybdenum and ferric reducing antioxidant power (FRAP) activities and antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli. When compared to AgNPs, fruit extract exhibited uppermost radical scavenging activities. In addition, the cytotoxicity activity was determined by MTT assay. Our results clearly proved that biosynthesized AgNPs inhibited proliferation of HeLA cell line with an IC50 of 87.32 ± 1.43 μg/mL and antibacterial activity. |
| format | Article |
| id | doaj-art-c65f2e403f414f16be36903fd94dcc11 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c65f2e403f414f16be36903fd94dcc112025-02-03T10:35:41ZengTsinghua University PressFood Science and Human Wellness2213-45302016-12-015420721810.1016/j.fshw.2016.10.001Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extractsG. MahendranB.D. Ranjitha KumariIn the present investigation, we have described the green biosynthesis of silver nanoparticles (AgNPs) using ripened fruit aqueous extract of Nothapodytes nimmoniana (Graham) Mabb. as capping agent. The antioxidant, anticancer and antimicrobial activities of AgNPs were also studied. UV analysis revealed that AgNPs had a sharp peak at 416 nm. X-ray diffraction (XRD) result confirmed the characteristic peaks indicated at 1 1 1, 2 0 0, 2 2 0 and 3 1 1 for the crystalline of the face centered cubic silver. The Scanning Electron Microscopy analysis results confirmed the spherical shaped of AgNPs with difference sizes of the particles and an average from 44 to 64 nm. Further, fruit extract and AgNPs were evaluated total phenolic, tannin and flavonoid contents and were subjected to assess their antioxidant potential using various in vitro systems such as using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), metal chelating, phosphomolybdenum and ferric reducing antioxidant power (FRAP) activities and antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli. When compared to AgNPs, fruit extract exhibited uppermost radical scavenging activities. In addition, the cytotoxicity activity was determined by MTT assay. Our results clearly proved that biosynthesized AgNPs inhibited proliferation of HeLA cell line with an IC50 of 87.32 ± 1.43 μg/mL and antibacterial activity.http://www.sciencedirect.com/science/article/pii/S2213453016301409Silver nanoparticlesGreen synthesisCytotoxicityAntioxidantDPPHFRAPXRDNothapodytes nimmoniana |
| spellingShingle | G. Mahendran B.D. Ranjitha Kumari Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts Food Science and Human Wellness Silver nanoparticles Green synthesis Cytotoxicity Antioxidant DPPH FRAP XRD Nothapodytes nimmoniana |
| title | Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts |
| title_full | Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts |
| title_fullStr | Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts |
| title_full_unstemmed | Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts |
| title_short | Biological activities of silver nanoparticles from Nothapodytes nimmoniana (Graham) Mabb. fruit extracts |
| title_sort | biological activities of silver nanoparticles from nothapodytes nimmoniana graham mabb fruit extracts |
| topic | Silver nanoparticles Green synthesis Cytotoxicity Antioxidant DPPH FRAP XRD Nothapodytes nimmoniana |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016301409 |
| work_keys_str_mv | AT gmahendran biologicalactivitiesofsilvernanoparticlesfromnothapodytesnimmonianagrahammabbfruitextracts AT bdranjithakumari biologicalactivitiesofsilvernanoparticlesfromnothapodytesnimmonianagrahammabbfruitextracts |