Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects
<i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza)</i> is one of the most commonly used bulk herbs in China; however, root rot can seriously affect its quality and yields. To minimize the use of chemical pesticides for managing this plant fungal disease, biological control...
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2025-01-01
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| author | Mingli Han Jing Wang Hang Lin Chen Yang Jing Zhang Jiawei Liu Guojun Pan Na Xiao |
| author_facet | Mingli Han Jing Wang Hang Lin Chen Yang Jing Zhang Jiawei Liu Guojun Pan Na Xiao |
| author_sort | Mingli Han |
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| description | <i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza)</i> is one of the most commonly used bulk herbs in China; however, root rot can seriously affect its quality and yields. To minimize the use of chemical pesticides for managing this plant fungal disease, biological control utilizing microbial bio-pesticides offers a promising alternative. This study aimed to enhance the biocontrol resources available for combating <i>S. miltiorrhiza</i> root rot by isolating actinomycetes with antifungal activity from the rhizosphere soil of <i>S. miltiorrhiza</i> and identifying biocontrol actinomycetes with a preventive effect on root rot. A total of 35 actinomycetes were successfully screened from the rhizosphere soil of <i>S. miltiorrhizae.</i> The strain B-35 with the strongest antifungal activity was screened out through antagonizing the pathogen <i>Fusarium solani</i> of <i>S. miltiorrhiza</i>, strain morphology and 16S rRNA analysis. The antagonistic actinomycetes fermentation filtrate and crude extract could significantly destroy the mycelium and spores of <i>Fusarium solani</i>; the biocontrol effect of mature <i>S. miltiorrhiza</i> rhizome reached 83.3%, and the number of leaves, plant height and biomass in the B-35 treatment group were significantly increased compared with the control group. B-35 has a certain application potential in the biological control of root rot and the promotion of <i>S. miltiorrhizae</i>. The antifungal activity of actinomycetes sourced from the rhizosphere soil of <i>S. miltiorrhiza</i> has been demonstrated for the first time, potentially enhancing future crop quality and production. |
| format | Article |
| id | doaj-art-c63f6e65f96846859d5c03d29a6ae272 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-c63f6e65f96846859d5c03d29a6ae2722025-01-24T13:16:43ZengMDPI AGAgronomy2073-43952025-01-0115110210.3390/agronomy15010102Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting EffectsMingli Han0Jing Wang1Hang Lin2Chen Yang3Jing Zhang4Jiawei Liu5Guojun Pan6Na Xiao7College of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, ChinaCollege of Life Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an 271000, ChinaCollege of Agronomy, Shandong Agriculture University, Tai’an 271018, China<i>Salvia miltiorrhiza</i> Bunge (<i>S. miltiorrhiza)</i> is one of the most commonly used bulk herbs in China; however, root rot can seriously affect its quality and yields. To minimize the use of chemical pesticides for managing this plant fungal disease, biological control utilizing microbial bio-pesticides offers a promising alternative. This study aimed to enhance the biocontrol resources available for combating <i>S. miltiorrhiza</i> root rot by isolating actinomycetes with antifungal activity from the rhizosphere soil of <i>S. miltiorrhiza</i> and identifying biocontrol actinomycetes with a preventive effect on root rot. A total of 35 actinomycetes were successfully screened from the rhizosphere soil of <i>S. miltiorrhizae.</i> The strain B-35 with the strongest antifungal activity was screened out through antagonizing the pathogen <i>Fusarium solani</i> of <i>S. miltiorrhiza</i>, strain morphology and 16S rRNA analysis. The antagonistic actinomycetes fermentation filtrate and crude extract could significantly destroy the mycelium and spores of <i>Fusarium solani</i>; the biocontrol effect of mature <i>S. miltiorrhiza</i> rhizome reached 83.3%, and the number of leaves, plant height and biomass in the B-35 treatment group were significantly increased compared with the control group. B-35 has a certain application potential in the biological control of root rot and the promotion of <i>S. miltiorrhizae</i>. The antifungal activity of actinomycetes sourced from the rhizosphere soil of <i>S. miltiorrhiza</i> has been demonstrated for the first time, potentially enhancing future crop quality and production.https://www.mdpi.com/2073-4395/15/1/102actinomycetes B-35antifungal activitybiocontrol<i>Salvia miltiorrhiza</i>plant growth promotion |
| spellingShingle | Mingli Han Jing Wang Hang Lin Chen Yang Jing Zhang Jiawei Liu Guojun Pan Na Xiao Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects Agronomy actinomycetes B-35 antifungal activity biocontrol <i>Salvia miltiorrhiza</i> plant growth promotion |
| title | Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects |
| title_full | Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects |
| title_fullStr | Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects |
| title_full_unstemmed | Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects |
| title_short | Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects |
| title_sort | screening and evaluation of actinomycetes b 35 from rhizosphere of i salvia miltiorrhiza i bunge and its biocontrol and growth promoting effects |
| topic | actinomycetes B-35 antifungal activity biocontrol <i>Salvia miltiorrhiza</i> plant growth promotion |
| url | https://www.mdpi.com/2073-4395/15/1/102 |
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