Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces
This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evalua...
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000556 |
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| author | Hui Zhang Xiaomei Guo Lei Tian Na Wang Yuqing Li Ariel Kushmaro Robert Marks Qun Sun |
| author_facet | Hui Zhang Xiaomei Guo Lei Tian Na Wang Yuqing Li Ariel Kushmaro Robert Marks Qun Sun |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay. DIM in the concentration range of 31.2−62.5 μmol/L demonstrated a dose-dependent antibiofilm activity to S. aureus, as confirmed by light microscopic (LM), confocal laser scanning microscopic (CLSM), and scanning electron microscopic (SEM) analyses. At DIM of 62.5 μmol/L, the biomass of S. aureus biofilm was significantly reduced by 97 % and its average thickness by 58 % (P < 0.05). DIM of 62.5 μmol/L inhibited the bacterial initial adhesion and proliferation, as well as cell motility; the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) were reduced by 75 % and 69 %, respectively. DIM exhibited a strong inhibition to S. aureus biofilm formation on common food-contact surfaces, including 304 stainless steel, glass, and polyvinyl chloride (PVC) but not disperse the mature biofilm. Overall, our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S. aureus on common food-contact surfaces utilized during food processing. |
| format | Article |
| id | doaj-art-c5fbbb87cad14bb4899fd6abc6cfc2e7 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c5fbbb87cad14bb4899fd6abc6cfc2e72025-02-03T02:27:47ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111512221232Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfacesHui Zhang0Xiaomei Guo1Lei Tian2Na Wang3Yuqing Li4Ariel Kushmaro5Robert Marks6Qun Sun7College of Biomass Science and Engineering, Sichuan University, Chengdu 610064, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610065, ChinaAvram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer- Sheva 84105, Israel; The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelAvram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer- Sheva 84105, Israel; The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelKey Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; Corresponding author at: College of Life Sciences, Sichuan University, Chengdu 610064, China,This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay. DIM in the concentration range of 31.2−62.5 μmol/L demonstrated a dose-dependent antibiofilm activity to S. aureus, as confirmed by light microscopic (LM), confocal laser scanning microscopic (CLSM), and scanning electron microscopic (SEM) analyses. At DIM of 62.5 μmol/L, the biomass of S. aureus biofilm was significantly reduced by 97 % and its average thickness by 58 % (P < 0.05). DIM of 62.5 μmol/L inhibited the bacterial initial adhesion and proliferation, as well as cell motility; the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) were reduced by 75 % and 69 %, respectively. DIM exhibited a strong inhibition to S. aureus biofilm formation on common food-contact surfaces, including 304 stainless steel, glass, and polyvinyl chloride (PVC) but not disperse the mature biofilm. Overall, our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S. aureus on common food-contact surfaces utilized during food processing.http://www.sciencedirect.com/science/article/pii/S2213453022000556Biofilm inhibition3,3'-DiindolylmethaneExtracellular DNAExtracellular polysaccharideFood-contact surfaces |
| spellingShingle | Hui Zhang Xiaomei Guo Lei Tian Na Wang Yuqing Li Ariel Kushmaro Robert Marks Qun Sun Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces Food Science and Human Wellness Biofilm inhibition 3,3'-Diindolylmethane Extracellular DNA Extracellular polysaccharide Food-contact surfaces |
| title | Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces |
| title_full | Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces |
| title_fullStr | Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces |
| title_full_unstemmed | Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces |
| title_short | Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces |
| title_sort | antibiofilm activity of 3 3 diindolylmethane on staphylococcus aureus and its disinfection on common food contact surfaces |
| topic | Biofilm inhibition 3,3'-Diindolylmethane Extracellular DNA Extracellular polysaccharide Food-contact surfaces |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000556 |
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