Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2
Seahorses are specialized teleosts due to their unique male pregnancy and reproductive physiology. In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. However, the molecular mechanism of germ cell development in seahorses remains unkn...
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772735124000842 |
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| author | Lingzhen Chen Lu Tang Qiang Lin Yanhong Zhang |
| author_facet | Lingzhen Chen Lu Tang Qiang Lin Yanhong Zhang |
| author_sort | Lingzhen Chen |
| collection | DOAJ |
| description | Seahorses are specialized teleosts due to their unique male pregnancy and reproductive physiology. In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. However, the molecular mechanism of germ cell development in seahorses remains unknown. Nanos, which encodes conserved zinc-finger RNA-binding proteins, play essential roles for germline development in both vertebrates and invertebrates. In this study, we characterized the nanos gene family members and their potential roles in germline development in the lined seahorse, Hippocampus erectus. We showed that the nanos family members in the seahorse are the nanos1a, nanos1b, and nanos3 genes, while nanos2, a key germline stem cell (GSC) maintenance factor, is intriguingly absent. More interestingly, nanos3, which is reported to be a female-specific gene, was also highly expressed in male gonads, as well as in the early developmental stages of testes and ovaries. Our results also showed that nanos3 was highly expressed in early meiotic germ cells, and that expression levels of nanos3 were lower in the gonad after TBT exposure. These findings suggest that nanos3 may function during male germ cell development, and in female initial germ cell production – processes which are regulated by nanos2 in other teleosts. This is the first research into how nanos governs germ cell development in an ovoviviparous fish. Our study provides insights into the regulatory mechanisms of germ cells development, as well as the early events during gametogenesis in the family Syngnathidae. |
| format | Article |
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| institution | Kabale University |
| issn | 2772-7351 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
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| series | Water Biology and Security |
| spelling | doaj-art-c5ef7a2e4dc342dba94bab900860e3232025-01-27T04:22:40ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512025-01-0141100312Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2Lingzhen Chen0Lu Tang1Qiang Lin2Yanhong Zhang3CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Corresponding author. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Corresponding author. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.Seahorses are specialized teleosts due to their unique male pregnancy and reproductive physiology. In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. However, the molecular mechanism of germ cell development in seahorses remains unknown. Nanos, which encodes conserved zinc-finger RNA-binding proteins, play essential roles for germline development in both vertebrates and invertebrates. In this study, we characterized the nanos gene family members and their potential roles in germline development in the lined seahorse, Hippocampus erectus. We showed that the nanos family members in the seahorse are the nanos1a, nanos1b, and nanos3 genes, while nanos2, a key germline stem cell (GSC) maintenance factor, is intriguingly absent. More interestingly, nanos3, which is reported to be a female-specific gene, was also highly expressed in male gonads, as well as in the early developmental stages of testes and ovaries. Our results also showed that nanos3 was highly expressed in early meiotic germ cells, and that expression levels of nanos3 were lower in the gonad after TBT exposure. These findings suggest that nanos3 may function during male germ cell development, and in female initial germ cell production – processes which are regulated by nanos2 in other teleosts. This is the first research into how nanos governs germ cell development in an ovoviviparous fish. Our study provides insights into the regulatory mechanisms of germ cells development, as well as the early events during gametogenesis in the family Syngnathidae.http://www.sciencedirect.com/science/article/pii/S2772735124000842Hippocampus erectusOvaryTestisNanosGerm cell developmentGametogenesis |
| spellingShingle | Lingzhen Chen Lu Tang Qiang Lin Yanhong Zhang Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 Water Biology and Security Hippocampus erectus Ovary Testis Nanos Germ cell development Gametogenesis |
| title | Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| title_full | Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| title_fullStr | Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| title_full_unstemmed | Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| title_short | Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| title_sort | seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 |
| topic | Hippocampus erectus Ovary Testis Nanos Germ cell development Gametogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2772735124000842 |
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