Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity
Silver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified from Euphorbia (Euphorbia amygdaloides) plant. Optimum pH, temperatur...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/486948 |
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| author | Semra Cicek Azize Alaylı Gungor Ahmet Adiguzel Hayrunnisa Nadaroglu |
| author_facet | Semra Cicek Azize Alaylı Gungor Ahmet Adiguzel Hayrunnisa Nadaroglu |
| author_sort | Semra Cicek |
| collection | DOAJ |
| description | Silver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified from Euphorbia (Euphorbia amygdaloides) plant. Optimum pH, temperature and time period were determined to obtain silver nanoparticles using the peroxidase enzyme. The result shows that higher silver nanoparticle was synthesized for 4 hours and at 20°C and pH 8. Also, optimal concentration of metal ions was found as 0.5 mM. The synthesized silver nanoparticles were characterized by UV spectrum, scanning electron microscope (SEM) and X-ray diffraction. Antibacterial activity of silver nanoparticles was measured against some microorganisms such as Serratia marcescens, Yersinia pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. Synthesized silver nanoparticles have wide spectrum antibacterial activity in low concentration and may be a good alternative therapeutic approach in medicine and pharmaceutical fields in future. |
| format | Article |
| id | doaj-art-c5eaa83bb39b4246916dfaaa73f7606c |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-c5eaa83bb39b4246916dfaaa73f7606c2025-02-03T01:03:29ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/486948486948Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial ActivitySemra Cicek0Azize Alaylı Gungor1Ahmet Adiguzel2Hayrunnisa Nadaroglu3Department of Nanoscience and Nanoengineering, Faculty of Engineering, Ataturk University, 25240 Erzurum, TurkeyDepartment of Chemical Technology, Erzurum Vocational Training School, Ataturk University, 25240 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240 Erzurum, TurkeyDepartment of Nanoscience and Nanoengineering, Faculty of Engineering, Ataturk University, 25240 Erzurum, TurkeySilver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified from Euphorbia (Euphorbia amygdaloides) plant. Optimum pH, temperature and time period were determined to obtain silver nanoparticles using the peroxidase enzyme. The result shows that higher silver nanoparticle was synthesized for 4 hours and at 20°C and pH 8. Also, optimal concentration of metal ions was found as 0.5 mM. The synthesized silver nanoparticles were characterized by UV spectrum, scanning electron microscope (SEM) and X-ray diffraction. Antibacterial activity of silver nanoparticles was measured against some microorganisms such as Serratia marcescens, Yersinia pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. Synthesized silver nanoparticles have wide spectrum antibacterial activity in low concentration and may be a good alternative therapeutic approach in medicine and pharmaceutical fields in future.http://dx.doi.org/10.1155/2015/486948 |
| spellingShingle | Semra Cicek Azize Alaylı Gungor Ahmet Adiguzel Hayrunnisa Nadaroglu Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity Journal of Chemistry |
| title | Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity |
| title_full | Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity |
| title_fullStr | Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity |
| title_full_unstemmed | Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity |
| title_short | Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity |
| title_sort | biochemical evaluation and green synthesis of nano silver using peroxidase from euphorbia euphorbia amygdaloides and its antibacterial activity |
| url | http://dx.doi.org/10.1155/2015/486948 |
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