Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis
Aim: For the determination of genetic structure of Acipayam Sheep, two enzyme loci were studied by using starch gel electrophoresis; carbonic anhidrase 1 (CA 1) and malic enzyme (ME). Materials and Methods: In this study, blood samples were taken from 50 sheeps in "Denizli Acıpayam State Farm&...
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=582 |
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| author | Ümmühan Özdemir Mehmet Nizamlıoğlu |
| author_facet | Ümmühan Özdemir Mehmet Nizamlıoğlu |
| author_sort | Ümmühan Özdemir |
| collection | DOAJ |
| description | Aim: For the determination of genetic structure of Acipayam Sheep, two enzyme loci were studied by using starch gel electrophoresis; carbonic anhidrase 1 (CA 1) and malic enzyme (ME).
Materials and Methods: In this study, blood samples were taken from 50 sheeps in "Denizli Acıpayam State Farm". Hemolysates were examined by electrophoresis. Tris-ED-TA-Borat pH:8.6 buffers were used for both of the enzymes. In staining after electrophoresis, MUA, acetone, agar noble, phosphate buffer pH:6.5 was used for carbonic anhidrase 1. NADP, MTT, PMS, DL Malic acid, MgCl2, NaOH, agar noble, Tris-HCl pH:8.0 buffer was used for malic enzyme. Bands were observed in ultraviolet lamps for CA 1 and appropriate light condition for ME.
Results: No polymorphism was observed for the CA 1 enzyme. All sheep were found to be homozygote for the same allele. For ME loci, population were observed polymorphism, having F, FS and S bands, the difference between F and S bands in ME loci were unimportant (p>0.05) and allels were determined in the Hardy-Weinberg equilibrium.
Conclusion: In this study, ME polymorphism was observed but no polymorphism was determined for CA 1 locus. It was concluded that it is necessary to investigate other enzymes in terms of polymorphism. |
| format | Article |
| id | doaj-art-c5a76178652741fc95e953618b3ccaca |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-c5a76178652741fc95e953618b3ccaca2025-02-03T11:25:40ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-1953273139143582Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresisÜmmühan Özdemir0Mehmet Nizamlıoğlu1İbrahim Hakkı Konyalı İlköğretim Okulu Fen ve Teknoloji Öğretmeni, Meram, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampus, Konya, TürkiyeAim: For the determination of genetic structure of Acipayam Sheep, two enzyme loci were studied by using starch gel electrophoresis; carbonic anhidrase 1 (CA 1) and malic enzyme (ME). Materials and Methods: In this study, blood samples were taken from 50 sheeps in "Denizli Acıpayam State Farm". Hemolysates were examined by electrophoresis. Tris-ED-TA-Borat pH:8.6 buffers were used for both of the enzymes. In staining after electrophoresis, MUA, acetone, agar noble, phosphate buffer pH:6.5 was used for carbonic anhidrase 1. NADP, MTT, PMS, DL Malic acid, MgCl2, NaOH, agar noble, Tris-HCl pH:8.0 buffer was used for malic enzyme. Bands were observed in ultraviolet lamps for CA 1 and appropriate light condition for ME. Results: No polymorphism was observed for the CA 1 enzyme. All sheep were found to be homozygote for the same allele. For ME loci, population were observed polymorphism, having F, FS and S bands, the difference between F and S bands in ME loci were unimportant (p>0.05) and allels were determined in the Hardy-Weinberg equilibrium. Conclusion: In this study, ME polymorphism was observed but no polymorphism was determined for CA 1 locus. It was concluded that it is necessary to investigate other enzymes in terms of polymorphism.http://eurasianjvetsci.org/pdf.php3?id=582acipayam sheepelectrophoresisgeneticpolymorphism |
| spellingShingle | Ümmühan Özdemir Mehmet Nizamlıoğlu Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis Eurasian Journal of Veterinary Sciences acipayam sheep electrophoresis genetic polymorphism |
| title | Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis |
| title_full | Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis |
| title_fullStr | Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis |
| title_full_unstemmed | Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis |
| title_short | Investigation of carbonic anhidrase 1 (ca 1) and malic enzyme (me) in acıpayam sheep by using starch gel electrophoresis |
| title_sort | investigation of carbonic anhidrase 1 ca 1 and malic enzyme me in acipayam sheep by using starch gel electrophoresis |
| topic | acipayam sheep electrophoresis genetic polymorphism |
| url | http://eurasianjvetsci.org/pdf.php3?id=582 |
| work_keys_str_mv | AT ummuhanozdemir investigationofcarbonicanhidrase1ca1andmalicenzymemeinacıpayamsheepbyusingstarchgelelectrophoresis AT mehmetnizamlıoglu investigationofcarbonicanhidrase1ca1andmalicenzymemeinacıpayamsheepbyusingstarchgelelectrophoresis |