1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides
A heteropolysaccharide, named L2, from Lentinula edodes has been proved to possess immunostimulating and anti-ageing activities in previous studies, but its acting mechanism was not completely understood. In this study, 1H NMR spectroscopy approach was employed to investigate the metabolic profiles...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2018-09-01
|
| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/pjfns.2018.68.issue-3/pjfns-2017-0029/pjfns-2017-0029.xml?format=INT |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562636924911616 |
|---|---|
| author | Xu Xiaofei Yang Jiguo Ning Zhengxiang Zhang Xuewu |
| author_facet | Xu Xiaofei Yang Jiguo Ning Zhengxiang Zhang Xuewu |
| author_sort | Xu Xiaofei |
| collection | DOAJ |
| description | A heteropolysaccharide, named L2, from Lentinula edodes has been proved to possess immunostimulating and anti-ageing activities in previous studies, but its acting mechanism was not completely understood. In this study, 1H NMR spectroscopy approach was employed to investigate the metabolic profiles of the urine from adult mice after L2 intervention. Using principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA), 22 potential biomarkers were found to be mainly involved in some metabolic pathways: amino acid metabolism, energy metabolism, lipid metabolism, tricarboxylic acid (TCA) cycle, urea cycle and gut microbiota metabolism. Among them, the significantly altered metabolites include: elevated glutamate (75%) and creatine (64%); decreased proline (65%), betaine (58%), fucose (63%) and dimethylamine (59%). In conclusion, the present data is helpful to understand the mechanisms related to previously confirmed immunomodulation and anti-aging effects of L2, and provide valuable information for mining new functions of L2. |
| format | Article |
| id | doaj-art-c581330f1d864226bbd0c87b143f31d9 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-c581330f1d864226bbd0c87b143f31d92025-02-03T01:22:18ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072018-09-0168320721610.1515/pjfns-2017-0029pjfns-2017-00291H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived PolysaccharidesXu Xiaofei0Yang Jiguo1Ning Zhengxiang2Zhang Xuewu3College of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou510640, ChinaCollege of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou510640, ChinaCollege of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou510640, ChinaCollege of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou510640, ChinaA heteropolysaccharide, named L2, from Lentinula edodes has been proved to possess immunostimulating and anti-ageing activities in previous studies, but its acting mechanism was not completely understood. In this study, 1H NMR spectroscopy approach was employed to investigate the metabolic profiles of the urine from adult mice after L2 intervention. Using principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA), 22 potential biomarkers were found to be mainly involved in some metabolic pathways: amino acid metabolism, energy metabolism, lipid metabolism, tricarboxylic acid (TCA) cycle, urea cycle and gut microbiota metabolism. Among them, the significantly altered metabolites include: elevated glutamate (75%) and creatine (64%); decreased proline (65%), betaine (58%), fucose (63%) and dimethylamine (59%). In conclusion, the present data is helpful to understand the mechanisms related to previously confirmed immunomodulation and anti-aging effects of L2, and provide valuable information for mining new functions of L2.http://www.degruyter.com/view/j/pjfns.2018.68.issue-3/pjfns-2017-0029/pjfns-2017-0029.xml?format=INTLentinula edodespolysaccharidemetabolomicsNMR spectroscopymetabolic pathways |
| spellingShingle | Xu Xiaofei Yang Jiguo Ning Zhengxiang Zhang Xuewu 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides Polish Journal of Food and Nutrition Sciences Lentinula edodes polysaccharide metabolomics NMR spectroscopy metabolic pathways |
| title | 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides |
| title_full | 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides |
| title_fullStr | 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides |
| title_full_unstemmed | 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides |
| title_short | 1H NMR-Based Metabolic Profiling of Urine from Mice Fed Lentinula edodes-Derived Polysaccharides |
| title_sort | 1h nmr based metabolic profiling of urine from mice fed lentinula edodes derived polysaccharides |
| topic | Lentinula edodes polysaccharide metabolomics NMR spectroscopy metabolic pathways |
| url | http://www.degruyter.com/view/j/pjfns.2018.68.issue-3/pjfns-2017-0029/pjfns-2017-0029.xml?format=INT |
| work_keys_str_mv | AT xuxiaofei 1hnmrbasedmetabolicprofilingofurinefrommicefedlentinulaedodesderivedpolysaccharides AT yangjiguo 1hnmrbasedmetabolicprofilingofurinefrommicefedlentinulaedodesderivedpolysaccharides AT ningzhengxiang 1hnmrbasedmetabolicprofilingofurinefrommicefedlentinulaedodesderivedpolysaccharides AT zhangxuewu 1hnmrbasedmetabolicprofilingofurinefrommicefedlentinulaedodesderivedpolysaccharides |