The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats
Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasinginsulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT)and moderate-intensity continuous training (MICT) exercises a...
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Royan Institute (ACECR), Tehran
2024-09-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_718758_17d4195e343538f79d799f55eb038e7f.pdf |
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| author | Mojdeh Khajehlandi Lotfali Bolboli |
| author_facet | Mojdeh Khajehlandi Lotfali Bolboli |
| author_sort | Mojdeh Khajehlandi |
| collection | DOAJ |
| description | Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasinginsulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT)and moderate-intensity continuous training (MICT) exercises along with quercetin administration on apoptosis andcardiomyopathy in diabetic obese rats.Materials and Methods: In this experimental study, 35 male Wistar rats [diabetic rats for experimental groups andnormal rats for healthy control (HC)] were divided into seven groups (for each group n=5): HC, diabetic control (DC),diabetic quercetin control (DQC), diabetic HIIT (DHT), diabetic MICT (DMT), DHT with quercetin (DQHT) and DMTwith quercetin (DQMT). The rats were fed a high-fat diet (HFD) for 8 weeks and a low dose of streptozotocin (STZ)was administered to create a model of type 2 diabetes mellitus (T2DM). Eight weeks of HIIT and MICT with or withoutquercetin treatment were performed. Quercetin was used at 15 mg/kg, as a suspension in carboxymethyl cellulose(CMC) at a concentration of 0.5%. One-way analysis of variance with LSD’s post-hoc test with a significant level ofP≤0.05 was used to analyze data.Results: Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (Casp-8) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, whileimproving inflammatory markers and the lipid profile (P<0.05).Conclusion: Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile byquercetin injection may be a promising approach for the development of new antidiabetic medications. In addition, bothtraining protocols showed potentially successful diabetic cardiomyopathy treatments through modulating the FOXO3and PI3K/AKT pathways. |
| format | Article |
| id | doaj-art-c56d0b730f3a4bafab8c1e838ae94581 |
| institution | Kabale University |
| issn | 2228-5806 2228-5814 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj-art-c56d0b730f3a4bafab8c1e838ae945812025-01-19T09:34:57ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142024-09-0126955956810.22074/cellj.2024.2042044.1682718758The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese RatsMojdeh Khajehlandi0Lotfali Bolboli1Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, IranDepartment of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, IranObjective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasinginsulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT)and moderate-intensity continuous training (MICT) exercises along with quercetin administration on apoptosis andcardiomyopathy in diabetic obese rats.Materials and Methods: In this experimental study, 35 male Wistar rats [diabetic rats for experimental groups andnormal rats for healthy control (HC)] were divided into seven groups (for each group n=5): HC, diabetic control (DC),diabetic quercetin control (DQC), diabetic HIIT (DHT), diabetic MICT (DMT), DHT with quercetin (DQHT) and DMTwith quercetin (DQMT). The rats were fed a high-fat diet (HFD) for 8 weeks and a low dose of streptozotocin (STZ)was administered to create a model of type 2 diabetes mellitus (T2DM). Eight weeks of HIIT and MICT with or withoutquercetin treatment were performed. Quercetin was used at 15 mg/kg, as a suspension in carboxymethyl cellulose(CMC) at a concentration of 0.5%. One-way analysis of variance with LSD’s post-hoc test with a significant level ofP≤0.05 was used to analyze data.Results: Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (Casp-8) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, whileimproving inflammatory markers and the lipid profile (P<0.05).Conclusion: Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile byquercetin injection may be a promising approach for the development of new antidiabetic medications. In addition, bothtraining protocols showed potentially successful diabetic cardiomyopathy treatments through modulating the FOXO3and PI3K/AKT pathways.https://www.celljournal.org/article_718758_17d4195e343538f79d799f55eb038e7f.pdfapoptosisdiabetesexerciseheartquercetin |
| spellingShingle | Mojdeh Khajehlandi Lotfali Bolboli The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats Cell Journal apoptosis diabetes exercise heart quercetin |
| title | The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats |
| title_full | The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats |
| title_fullStr | The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats |
| title_full_unstemmed | The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats |
| title_short | The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats |
| title_sort | role of quercetin and exercise in modulating apoptosis and cardiomyopathy via pi3k akt foxo3 pathways in diabetic obese rats |
| topic | apoptosis diabetes exercise heart quercetin |
| url | https://www.celljournal.org/article_718758_17d4195e343538f79d799f55eb038e7f.pdf |
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