Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate
A fluorescence resonance energy transfer (FRET)- based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′- FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH...
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| Format: | Article |
| Language: | English |
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Wiley
2016-05-01
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| Series: | Nanomaterials and Nanotechnology |
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| Online Access: | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/selective-and-sensitive-fluorescence-aptamer-biosensors-of-adenosine-triphosphate |
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| author | Chao Meng Zhao Dai Wenjuan Guo Yuanyuan Chu Guangping Chen |
| author_facet | Chao Meng Zhao Dai Wenjuan Guo Yuanyuan Chu Guangping Chen |
| author_sort | Chao Meng |
| collection | DOAJ |
| description | A fluorescence resonance energy transfer (FRET)- based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′- FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH-DNA by Au-S bond. This aptasensor was obtained by the hybridization between the complementary DNA strands of the 5′-FAM and AuNPs. The detecting results indicated that the FRET increase efficiency (Fr) of detecting system at 519 nm was linear when the concentration of ATP target was about 0.1–10 mmol/L. Moreover, the aptasensors showed a good stability in different buffer conditions and a low- detection limit (15.2 nmol/L) for ATP. |
| format | Article |
| id | doaj-art-c567a97869214e5b84cb3b4034f38c0c |
| institution | Kabale University |
| issn | 1847-9804 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Nanomaterials and Nanotechnology |
| spelling | doaj-art-c567a97869214e5b84cb3b4034f38c0c2025-02-03T06:42:54ZengWileyNanomaterials and Nanotechnology1847-98042016-05-01633http://dx.doi.org/10.5772/6398550667Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine TriphosphateChao MengZhao DaiWenjuan GuoYuanyuan ChuGuangping ChenA fluorescence resonance energy transfer (FRET)- based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′- FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH-DNA by Au-S bond. This aptasensor was obtained by the hybridization between the complementary DNA strands of the 5′-FAM and AuNPs. The detecting results indicated that the FRET increase efficiency (Fr) of detecting system at 519 nm was linear when the concentration of ATP target was about 0.1–10 mmol/L. Moreover, the aptasensors showed a good stability in different buffer conditions and a low- detection limit (15.2 nmol/L) for ATP.http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/selective-and-sensitive-fluorescence-aptamer-biosensors-of-adenosine-triphosphateATPFluorescence Resonance Energy TransferBiosensorAptamerAptasensor |
| spellingShingle | Chao Meng Zhao Dai Wenjuan Guo Yuanyuan Chu Guangping Chen Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate Nanomaterials and Nanotechnology ATP Fluorescence Resonance Energy Transfer Biosensor Aptamer Aptasensor |
| title | Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate |
| title_full | Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate |
| title_fullStr | Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate |
| title_full_unstemmed | Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate |
| title_short | Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate |
| title_sort | selective and sensitive fluorescence aptamer biosensors of adenosine triphosphate |
| topic | ATP Fluorescence Resonance Energy Transfer Biosensor Aptamer Aptasensor |
| url | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/selective-and-sensitive-fluorescence-aptamer-biosensors-of-adenosine-triphosphate |
| work_keys_str_mv | AT chaomeng selectiveandsensitivefluorescenceaptamerbiosensorsofadenosinetriphosphate AT zhaodai selectiveandsensitivefluorescenceaptamerbiosensorsofadenosinetriphosphate AT wenjuanguo selectiveandsensitivefluorescenceaptamerbiosensorsofadenosinetriphosphate AT yuanyuanchu selectiveandsensitivefluorescenceaptamerbiosensorsofadenosinetriphosphate AT guangpingchen selectiveandsensitivefluorescenceaptamerbiosensorsofadenosinetriphosphate |