Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers
Aims: The purpose of this study was to detect potential cytokines markers involved in the Graves’ orbitopathy (GO). Materials and Methods: We measured 25 plasma cytokines using label-free quantitative proteomic analysis in the pilot cohort. In validation cohort, NORCH3, TBP, α-enolase (ENO1), GPX3,...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-04-01
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| Series: | Environmental Disease |
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| Online Access: | https://journals.lww.com/10.4103/ed.ed_3_24 |
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| _version_ | 1832590691864150016 |
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| author | Ying Fu Yuanyuan Zhang Yaxin An Bin Cao Dong Zhao |
| author_facet | Ying Fu Yuanyuan Zhang Yaxin An Bin Cao Dong Zhao |
| author_sort | Ying Fu |
| collection | DOAJ |
| description | Aims:
The purpose of this study was to detect potential cytokines markers involved in the Graves’ orbitopathy (GO).
Materials and Methods:
We measured 25 plasma cytokines using label-free quantitative proteomic analysis in the pilot cohort. In validation cohort, NORCH3, TBP, α-enolase (ENO1), GPX3, ORM1, extracellular matrix protein-1 (ECM1), SERPINE, IGF1, IGKV1, and SPARC were validated using ELISA kits. We used label-free liquid chromatography–mass spectrometry/MS (LC–MS/MS) quantitative proteomic to analyze plasma cytokines content of Graves’ disease and GO patients.
Results:
The validation cohort showed that the concentrations of ECM1, ENO1, and GPX3, (244.4276 pg/ml, 4.361807 ng/ml, and 17.72794 pmol/ml, respectively) were significantly lower in GO patients samples than those in Graves’ patients (257.3197 pg/ml, 4.601592 ng/ml, and 21.33118 pmol/ml, respectively). Both univariate and multivariate logistic analyses indicated that GPX3, ECM1, and ENO might be predictors of Graves’ ophthalmopathy. A receiver-operating characteristic curve with an area under the curve of 0.938, a sensitivity of 84.4%, and a specificity of 90.5% underscored the applicability of this method.
Conclusions:
ECM1, ENO1, and GPX3 were associated with the presence of the GO group and may be good biomarkers that play an important role in the pathogenesis of GO. |
| format | Article |
| id | doaj-art-c5637d304a3a401abd0d5914e37cdd91 |
| institution | Kabale University |
| issn | 2468-5704 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Environmental Disease |
| spelling | doaj-art-c5637d304a3a401abd0d5914e37cdd912025-01-23T09:09:46ZengWolters Kluwer Medknow PublicationsEnvironmental Disease2468-57042024-04-0192465210.4103/ed.ed_3_24Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markersYing FuYuanyuan ZhangYaxin AnBin CaoDong ZhaoAims: The purpose of this study was to detect potential cytokines markers involved in the Graves’ orbitopathy (GO). Materials and Methods: We measured 25 plasma cytokines using label-free quantitative proteomic analysis in the pilot cohort. In validation cohort, NORCH3, TBP, α-enolase (ENO1), GPX3, ORM1, extracellular matrix protein-1 (ECM1), SERPINE, IGF1, IGKV1, and SPARC were validated using ELISA kits. We used label-free liquid chromatography–mass spectrometry/MS (LC–MS/MS) quantitative proteomic to analyze plasma cytokines content of Graves’ disease and GO patients. Results: The validation cohort showed that the concentrations of ECM1, ENO1, and GPX3, (244.4276 pg/ml, 4.361807 ng/ml, and 17.72794 pmol/ml, respectively) were significantly lower in GO patients samples than those in Graves’ patients (257.3197 pg/ml, 4.601592 ng/ml, and 21.33118 pmol/ml, respectively). Both univariate and multivariate logistic analyses indicated that GPX3, ECM1, and ENO might be predictors of Graves’ ophthalmopathy. A receiver-operating characteristic curve with an area under the curve of 0.938, a sensitivity of 84.4%, and a specificity of 90.5% underscored the applicability of this method. Conclusions: ECM1, ENO1, and GPX3 were associated with the presence of the GO group and may be good biomarkers that play an important role in the pathogenesis of GO.https://journals.lww.com/10.4103/ed.ed_3_24graves’ orbitopathylabel-free liquid chromatography–mass spectrometry/mass spectrometryplasma proteinpotential markers |
| spellingShingle | Ying Fu Yuanyuan Zhang Yaxin An Bin Cao Dong Zhao Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers Environmental Disease graves’ orbitopathy label-free liquid chromatography–mass spectrometry/mass spectrometry plasma protein potential markers |
| title | Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers |
| title_full | Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers |
| title_fullStr | Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers |
| title_full_unstemmed | Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers |
| title_short | Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers |
| title_sort | changes of plasma cytokines content in graves orbitopathy patients could be potential markers |
| topic | graves’ orbitopathy label-free liquid chromatography–mass spectrometry/mass spectrometry plasma protein potential markers |
| url | https://journals.lww.com/10.4103/ed.ed_3_24 |
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