Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don
Allium cepa L. var. agrogatum Don (ALAP) is commonly consumed in China as well as other regions and has various beneficial health effects. A novel acidic polysaccharide (named ALAP-21) was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchang...
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| Language: | English |
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Tsinghua University Press
2020-03-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453019301375 |
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| author | Hongcheng Liu Hongxiu Fan Jing Zhang Shanshan Zhang Wenting Zhao Tingting Liu Dawei Wang |
| author_facet | Hongcheng Liu Hongxiu Fan Jing Zhang Shanshan Zhang Wenting Zhao Tingting Liu Dawei Wang |
| author_sort | Hongcheng Liu |
| collection | DOAJ |
| description | Allium cepa L. var. agrogatum Don (ALAP) is commonly consumed in China as well as other regions and has various beneficial health effects. A novel acidic polysaccharide (named ALAP-21) was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchange and Sephadex G-100 columns. The monosaccharide composition, structural and antioxidative properties of ALAP-21 were investigated by GC–MS chromatography, FT-IR and NMR spectroscopies and three antioxidative activity tests in vitro. The results showed that ALAP-21 was a heteropolysaccharide composed of glucose, galacturonic acid, mannose, galactose, arabinose, rhamnose, xylose, fructose and glucuronic acid with a relative molar ratio of 26.282: 27.546: 11.400: 4.781: 2.467: 2.445: 3.622: 1.106: 1.753, owning (1→4)-α-d-Glcp, (1→4)-α-d-GalAp6Me3OA, (1→4)-β-d-Galp6OMe, (1→2)-α-l-Rhap, (1→4)-β-d-Manp glycosidic linkages. (1→4)-α-d-Glcp and (1→4)-α-d-GalAp6Me3OA residues might be the main components of the sugar chain backbone of ALAP-21. Furthermore, ALAP-21 exhibited high potential for DPPH radicals (82.02%), hydroxyl radicals (53.33%) and superoxide anion radicals (50.28%). These results provide a reference for further research and rational development of ALAP polysaccharides. Keywords: Polysaccharides, Allium cepa L. var. agrogatum Don, Structural characterization, Antioxidative property |
| format | Article |
| id | doaj-art-c558316692924ee6ac01bc363fbeef7d |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c558316692924ee6ac01bc363fbeef7d2025-02-03T10:32:07ZengTsinghua University PressFood Science and Human Wellness2213-45302020-03-01917179Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum DonHongcheng Liu0Hongxiu Fan1Jing Zhang2Shanshan Zhang3Wenting Zhao4Tingting Liu5Dawei Wang6School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Scientific Research Base of Edible Mushroom Processing Technology Integration of Ministry of Agriculture and Rural Affairs, Changchun, 130118, ChinaSchool of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Engineering Research Center of Grain Deep-processing and High-effeciency Utilization of Jilin Province, Changchun, 130118, ChinaSchool of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Technological Innovations for Grain Deep-processing and High-effeciency Utilization of By-products of Jilin Province, Changchun, 130118, ChinaSchool of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Technological Innovations for Grain Deep-processing and High-effeciency Utilization of By-products of Jilin Province, Changchun, 130118, ChinaSchool of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Scientific Research Base of Edible Mushroom Processing Technology Integration of Ministry of Agriculture and Rural Affairs, Changchun, 130118, ChinaSchool of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Engineering Research Center of Grain Deep-processing and High-effeciency Utilization of Jilin Province, Changchun, 130118, China; Corresponding authors at: School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China.School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Scientific Research Base of Edible Mushroom Processing Technology Integration of Ministry of Agriculture and Rural Affairs, Changchun, 130118, China; Corresponding authors at: School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China.Allium cepa L. var. agrogatum Don (ALAP) is commonly consumed in China as well as other regions and has various beneficial health effects. A novel acidic polysaccharide (named ALAP-21) was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchange and Sephadex G-100 columns. The monosaccharide composition, structural and antioxidative properties of ALAP-21 were investigated by GC–MS chromatography, FT-IR and NMR spectroscopies and three antioxidative activity tests in vitro. The results showed that ALAP-21 was a heteropolysaccharide composed of glucose, galacturonic acid, mannose, galactose, arabinose, rhamnose, xylose, fructose and glucuronic acid with a relative molar ratio of 26.282: 27.546: 11.400: 4.781: 2.467: 2.445: 3.622: 1.106: 1.753, owning (1→4)-α-d-Glcp, (1→4)-α-d-GalAp6Me3OA, (1→4)-β-d-Galp6OMe, (1→2)-α-l-Rhap, (1→4)-β-d-Manp glycosidic linkages. (1→4)-α-d-Glcp and (1→4)-α-d-GalAp6Me3OA residues might be the main components of the sugar chain backbone of ALAP-21. Furthermore, ALAP-21 exhibited high potential for DPPH radicals (82.02%), hydroxyl radicals (53.33%) and superoxide anion radicals (50.28%). These results provide a reference for further research and rational development of ALAP polysaccharides. Keywords: Polysaccharides, Allium cepa L. var. agrogatum Don, Structural characterization, Antioxidative propertyhttp://www.sciencedirect.com/science/article/pii/S2213453019301375 |
| spellingShingle | Hongcheng Liu Hongxiu Fan Jing Zhang Shanshan Zhang Wenting Zhao Tingting Liu Dawei Wang Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don Food Science and Human Wellness |
| title | Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don |
| title_full | Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don |
| title_fullStr | Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don |
| title_full_unstemmed | Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don |
| title_short | Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don |
| title_sort | isolation purification structural characteristic and antioxidative property of polysaccharides from a cepa l var agrogatum don |
| url | http://www.sciencedirect.com/science/article/pii/S2213453019301375 |
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