Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts
Background: Platelet-rich fibrin (PRF) and Photobiomodulation (PBM) are established methods for promoting bone healing. PRF enhances cell proliferation and migration due to its rich concentration of growth factors, while PBM stimulates tissue repair through mitochondrial activation. Despite their ef...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004721 |
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| author | Ling-Hung Chou Tzu-Sen Yang Pei-Chun Wong Ying-Chun Chen |
| author_facet | Ling-Hung Chou Tzu-Sen Yang Pei-Chun Wong Ying-Chun Chen |
| author_sort | Ling-Hung Chou |
| collection | DOAJ |
| description | Background: Platelet-rich fibrin (PRF) and Photobiomodulation (PBM) are established methods for promoting bone healing. PRF enhances cell proliferation and migration due to its rich concentration of growth factors, while PBM stimulates tissue repair through mitochondrial activation. Despite their efficacies, no in-depth studies have explored the synergistic effects of combining PRF and PBM. Methods: PRF was prepared at 50 % and 100 % concentrations, and PBM was applied using an 830 nm near-infrared laser at a dose of 5 J/cm². Cell viability, migration, and calcium deposition were assessed over seven and fourteen days. Results: The combination of PRF and PBM significantly improved cell viability, migration, and calcium deposition, with the most notable effects observed after seven and fourteen days. However, a slight decrease in calcium deposition was noted in the 100 % PRF combined with the PBM group, suggesting a potential feedback mechanism at higher PRF concentrations. Conclusions: This study explores the synergistic effects of PRF and PBM, offering new insights into optimizing bone tissue engineering strategies. The findings highlight the potential of this combined approach in enhancing bone regeneration, although further research is needed to refine the optimal conditions for these therapies. |
| format | Article |
| id | doaj-art-c547406873ef48db991374d773b7a5cf |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-c547406873ef48db991374d773b7a5cf2025-02-01T04:11:42ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-02-0151104436Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 PreosteoblastsLing-Hung Chou0Tzu-Sen Yang1Pei-Chun Wong2Ying-Chun Chen3Department of Mechanical Engineering, National Taipei University of Technology, Taipei, TaiwanGraduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei, TaiwanGraduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei, TaiwanDepartment of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan; Corresponding author at: Department of Mechanical Engineering, National Taiwan University of Science and Technology, No.43, Keelung Rd., Sec.4, Da'an Dist., Taipei, 106335, Taiwan.Background: Platelet-rich fibrin (PRF) and Photobiomodulation (PBM) are established methods for promoting bone healing. PRF enhances cell proliferation and migration due to its rich concentration of growth factors, while PBM stimulates tissue repair through mitochondrial activation. Despite their efficacies, no in-depth studies have explored the synergistic effects of combining PRF and PBM. Methods: PRF was prepared at 50 % and 100 % concentrations, and PBM was applied using an 830 nm near-infrared laser at a dose of 5 J/cm². Cell viability, migration, and calcium deposition were assessed over seven and fourteen days. Results: The combination of PRF and PBM significantly improved cell viability, migration, and calcium deposition, with the most notable effects observed after seven and fourteen days. However, a slight decrease in calcium deposition was noted in the 100 % PRF combined with the PBM group, suggesting a potential feedback mechanism at higher PRF concentrations. Conclusions: This study explores the synergistic effects of PRF and PBM, offering new insights into optimizing bone tissue engineering strategies. The findings highlight the potential of this combined approach in enhancing bone regeneration, although further research is needed to refine the optimal conditions for these therapies.http://www.sciencedirect.com/science/article/pii/S1572100024004721Platelet-rich fibrinPhotobiomodulationBone regenerationMC3T3-E1 preosteoblastsSynergistic effectsLow level light |
| spellingShingle | Ling-Hung Chou Tzu-Sen Yang Pei-Chun Wong Ying-Chun Chen Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts Photodiagnosis and Photodynamic Therapy Platelet-rich fibrin Photobiomodulation Bone regeneration MC3T3-E1 preosteoblasts Synergistic effects Low level light |
| title | Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts |
| title_full | Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts |
| title_fullStr | Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts |
| title_full_unstemmed | Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts |
| title_short | Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts |
| title_sort | synergistic effects of platelet rich fibrin and photobiomodulation on bone regeneration in mc3t3 e1 preosteoblasts |
| topic | Platelet-rich fibrin Photobiomodulation Bone regeneration MC3T3-E1 preosteoblasts Synergistic effects Low level light |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004721 |
| work_keys_str_mv | AT linghungchou synergisticeffectsofplateletrichfibrinandphotobiomodulationonboneregenerationinmc3t3e1preosteoblasts AT tzusenyang synergisticeffectsofplateletrichfibrinandphotobiomodulationonboneregenerationinmc3t3e1preosteoblasts AT peichunwong synergisticeffectsofplateletrichfibrinandphotobiomodulationonboneregenerationinmc3t3e1preosteoblasts AT yingchunchen synergisticeffectsofplateletrichfibrinandphotobiomodulationonboneregenerationinmc3t3e1preosteoblasts |