First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China
ABSTRACT Background Carnivore parvovirus is commonly detected in canines and felines and results in a range of symptoms, including diarrhoea, with a high lethality rate. In recent years, the gradual restoration of urban ecosystems coupled with a lack of natural predators means that wild raccoon dogs...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70071 |
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| author | Chen Chen Jin Tao Ling Tang Tong Sun Zhuyun Sun Huan Xu Shuaitao Deng Cheng Xue Xing Ji Rui Wang Hongjun Chen |
| author_facet | Chen Chen Jin Tao Ling Tang Tong Sun Zhuyun Sun Huan Xu Shuaitao Deng Cheng Xue Xing Ji Rui Wang Hongjun Chen |
| author_sort | Chen Chen |
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| description | ABSTRACT Background Carnivore parvovirus is commonly detected in canines and felines and results in a range of symptoms, including diarrhoea, with a high lethality rate. In recent years, the gradual restoration of urban ecosystems coupled with a lack of natural predators means that wild raccoon dogs have gradually entered residential neighbourhoods in Shanghai, and the carnivore parvovirus carried by such canines may seriously affect their health and that of dogs and cats in these neighbourhoods. Objectives Therefore, it is essential to monitor wild raccoon dogs for carnivore parvovirus. Methods The wild raccoon samples were collected from the residential area of Shanghai and screened for parvovirus, followed by virus isolation and sequence comparison analysis. Results In this study, three strains of parvovirus, YSH1‐2022, YSH2‐2022 and GSW1‐2023, were isolated from anal and pharyngeal swab mixtures of 42 live raccoon dogs and two dead raccoon dogs in Shanghai, China. After alignment, it was found that the three strains of parvovirus were all located in the same branch as feline panleukopenia virus (FPV), and the three VP2 genes had 100% homology with FPV VP2 genes isolated from Shanghai and Yangzhou, Jiangsu Province. The homology of the three NS1 genes with FPV NS1 isolated from Shanghai and other places was 99.9%–100%. Conclusion This is the first report of FPV isolated from raccoon dogs. In this study, the host range and risk of infection of parvovirus were evaluated, providing data to support wildlife management and policy formulation in Shanghai. |
| format | Article |
| id | doaj-art-c545ab833d3f41d79431ced3241a3536 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-c545ab833d3f41d79431ced3241a35362025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70071First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, ChinaChen Chen0Jin Tao1Ling Tang2Tong Sun3Zhuyun Sun4Huan Xu5Shuaitao Deng6Cheng Xue7Xing Ji8Rui Wang9Hongjun Chen10Shanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaKey Laboratory of Animal Biosafety Risk Prevention and Control (North) of Ministry of Agriculture and Rural Affairs Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Shanghai ChinaKey Laboratory of Animal Biosafety Risk Prevention and Control (North) of Ministry of Agriculture and Rural Affairs Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Shanghai ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaJiangsu Key Laboratory for Food Quality and Safety—State Key Laboratory Cultivation Base of Ministry of Science and Technology Institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences Nanjing ChinaShanghai Wildlife and Protected Natural Areas Research Center Shanghai ChinaKey Laboratory of Animal Biosafety Risk Prevention and Control (North) of Ministry of Agriculture and Rural Affairs Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Shanghai ChinaABSTRACT Background Carnivore parvovirus is commonly detected in canines and felines and results in a range of symptoms, including diarrhoea, with a high lethality rate. In recent years, the gradual restoration of urban ecosystems coupled with a lack of natural predators means that wild raccoon dogs have gradually entered residential neighbourhoods in Shanghai, and the carnivore parvovirus carried by such canines may seriously affect their health and that of dogs and cats in these neighbourhoods. Objectives Therefore, it is essential to monitor wild raccoon dogs for carnivore parvovirus. Methods The wild raccoon samples were collected from the residential area of Shanghai and screened for parvovirus, followed by virus isolation and sequence comparison analysis. Results In this study, three strains of parvovirus, YSH1‐2022, YSH2‐2022 and GSW1‐2023, were isolated from anal and pharyngeal swab mixtures of 42 live raccoon dogs and two dead raccoon dogs in Shanghai, China. After alignment, it was found that the three strains of parvovirus were all located in the same branch as feline panleukopenia virus (FPV), and the three VP2 genes had 100% homology with FPV VP2 genes isolated from Shanghai and Yangzhou, Jiangsu Province. The homology of the three NS1 genes with FPV NS1 isolated from Shanghai and other places was 99.9%–100%. Conclusion This is the first report of FPV isolated from raccoon dogs. In this study, the host range and risk of infection of parvovirus were evaluated, providing data to support wildlife management and policy formulation in Shanghai.https://doi.org/10.1002/vms3.70071feline panleukopenia virusisolationphylogenetic analysisresidential arearisk analysiswild raccoon dogs |
| spellingShingle | Chen Chen Jin Tao Ling Tang Tong Sun Zhuyun Sun Huan Xu Shuaitao Deng Cheng Xue Xing Ji Rui Wang Hongjun Chen First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China Veterinary Medicine and Science feline panleukopenia virus isolation phylogenetic analysis residential area risk analysis wild raccoon dogs |
| title | First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China |
| title_full | First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China |
| title_fullStr | First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China |
| title_full_unstemmed | First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China |
| title_short | First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China |
| title_sort | first isolation and characterization of feline panleukopenia virus from wild raccoon dogs in the residential area of shanghai china |
| topic | feline panleukopenia virus isolation phylogenetic analysis residential area risk analysis wild raccoon dogs |
| url | https://doi.org/10.1002/vms3.70071 |
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