Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis
BackgroundGliomas are highly aggressive, life-threatening tumors with poor prognosis, and remain a leading cause of mortality among brain cancers. Although the role of mitochondrial proteins in cancer has garnered increasing attention, their specific functions in the nervous system, particularly in...
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Frontiers Media S.A.
2025-06-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1581850/full |
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| author | Yanyu Ding Yanyu Ding Lizhi Xiao Lizhi Xiao Xiaoling Zhou Xiaoling Zhou Jiaxin Zhao Jiaxin Zhao Jianli Ke Jianli Ke Huabao Cai Mengyu Zhao Cunzhi Wang Tianhang Yu Zhijie Zhao Yucai Wang Yucai Wang Yucai Wang Jiyuan Ke Jiyuan Ke |
| author_facet | Yanyu Ding Yanyu Ding Lizhi Xiao Lizhi Xiao Xiaoling Zhou Xiaoling Zhou Jiaxin Zhao Jiaxin Zhao Jianli Ke Jianli Ke Huabao Cai Mengyu Zhao Cunzhi Wang Tianhang Yu Zhijie Zhao Yucai Wang Yucai Wang Yucai Wang Jiyuan Ke Jiyuan Ke |
| author_sort | Yanyu Ding |
| collection | DOAJ |
| description | BackgroundGliomas are highly aggressive, life-threatening tumors with poor prognosis, and remain a leading cause of mortality among brain cancers. Although the role of mitochondrial proteins in cancer has garnered increasing attention, their specific functions in the nervous system, particularly in gliomas, remain poorly understood.MethodsWe integrated single-cell RNA sequencing with cellular assays and flow cytometry to investigate the molecular characteristics and cellular interactions within glioblastoma subpopulations during tumor progression.ResultsSingle-cell RNA sequencing revealed several differentially expressed genes (DEGs) within glioblastoma subpopulations. Trajectory analysis identified CHCHD2P9 as a pivotal marker for the terminal subpopulation. Moreover, elevated expression of CHCHD2P9 was found to correlate with poorer clinical outcomes. Subsequent cellular experiments further explored the underlying mechanisms driving these observations.ConclusionsCHCHD2P9 is significantly overexpressed in glioma patients, and its differential expression plays a crucial role in regulating glioma cell proliferation and migration. A CHCHD2P9-based risk model holds promise as both a prognostic biomarker and a potential therapeutic target, providing novel insights into the pathogenesis of gliomas and opening avenues for personalized treatment strategies. |
| format | Article |
| id | doaj-art-c52a6408edce4502ba7677d85f7a54e8 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-c52a6408edce4502ba7677d85f7a54e82025-08-20T02:21:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.15818501581850Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosisYanyu Ding0Yanyu Ding1Lizhi Xiao2Lizhi Xiao3Xiaoling Zhou4Xiaoling Zhou5Jiaxin Zhao6Jiaxin Zhao7Jianli Ke8Jianli Ke9Huabao Cai10Mengyu Zhao11Cunzhi Wang12Tianhang Yu13Zhijie Zhao14Yucai Wang15Yucai Wang16Yucai Wang17Jiyuan Ke18Jiyuan Ke19Department of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaThe Chinese Academy of Sciences (CAS) Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, ChinaDepartment of Immunology, School of Basic Medical Sciences, Center for Big Data and Population Health of Institute of Health and Medicine (IHM), Anhui Medical University, Hefei, ChinaInstitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei Economic and Technological Development Zone, Hefei, Anhui, ChinaBackgroundGliomas are highly aggressive, life-threatening tumors with poor prognosis, and remain a leading cause of mortality among brain cancers. Although the role of mitochondrial proteins in cancer has garnered increasing attention, their specific functions in the nervous system, particularly in gliomas, remain poorly understood.MethodsWe integrated single-cell RNA sequencing with cellular assays and flow cytometry to investigate the molecular characteristics and cellular interactions within glioblastoma subpopulations during tumor progression.ResultsSingle-cell RNA sequencing revealed several differentially expressed genes (DEGs) within glioblastoma subpopulations. Trajectory analysis identified CHCHD2P9 as a pivotal marker for the terminal subpopulation. Moreover, elevated expression of CHCHD2P9 was found to correlate with poorer clinical outcomes. Subsequent cellular experiments further explored the underlying mechanisms driving these observations.ConclusionsCHCHD2P9 is significantly overexpressed in glioma patients, and its differential expression plays a crucial role in regulating glioma cell proliferation and migration. A CHCHD2P9-based risk model holds promise as both a prognostic biomarker and a potential therapeutic target, providing novel insights into the pathogenesis of gliomas and opening avenues for personalized treatment strategies.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1581850/fullglioblastomaCHCHD2P9diagnosisprognosiscentral nervous systemtumor microenvironment |
| spellingShingle | Yanyu Ding Yanyu Ding Lizhi Xiao Lizhi Xiao Xiaoling Zhou Xiaoling Zhou Jiaxin Zhao Jiaxin Zhao Jianli Ke Jianli Ke Huabao Cai Mengyu Zhao Cunzhi Wang Tianhang Yu Zhijie Zhao Yucai Wang Yucai Wang Yucai Wang Jiyuan Ke Jiyuan Ke Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis Frontiers in Immunology glioblastoma CHCHD2P9 diagnosis prognosis central nervous system tumor microenvironment |
| title | Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis |
| title_full | Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis |
| title_fullStr | Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis |
| title_full_unstemmed | Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis |
| title_short | Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis |
| title_sort | molecular insights into glioblastoma progression role of chchd2p9 in tumor heterogeneity and prognosis |
| topic | glioblastoma CHCHD2P9 diagnosis prognosis central nervous system tumor microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1581850/full |
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