TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis
Abstract Background Sucrose non-fermenting-1-related protein kinases (SnRKs) have been implicated in plant growth and stress responses. Although SnRK3.23 is known to be involved in drought stress, the underlying mechanism of resistance differs between Arabidopsis and rice, and little is known about...
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BMC
2025-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06091-y |
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| author | Feiyan Dong Yide Liu Huadong Zhang Yaqian Li Jinghan Song Sheng Chen Shuailei Wang Zhanwang Zhu Yan Li Yike Liu |
| author_facet | Feiyan Dong Yide Liu Huadong Zhang Yaqian Li Jinghan Song Sheng Chen Shuailei Wang Zhanwang Zhu Yan Li Yike Liu |
| author_sort | Feiyan Dong |
| collection | DOAJ |
| description | Abstract Background Sucrose non-fermenting-1-related protein kinases (SnRKs) have been implicated in plant growth and stress responses. Although SnRK3.23 is known to be involved in drought stress, the underlying mechanism of resistance differs between Arabidopsis and rice, and little is known about its function in wheat. Results In the current work, TaSnRK3.23B was detected on the cell membrane and in the nucleus. TaSnRK3.23B overexpression in Arabidopsis promoted reactive oxygen species (ROS) scavenging via the accumulation of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and then conferred significant tolerance to drought. The prediction analysis, yeast two-hybrid, and bimolecular fluorescence complementation (BiFC) assays revealed that TaSnRK3.23B interacted with TaCBL2B and TaCBL6B, which are calcineurin B-like (CBL) proteins. The predicted model demonstrated that TaSnRK3 subfamily proteins participate in Ca2+ signaling mediated by TaCBL2B/TaCBL6B and subsequently provide drought stress tolerance by promoting ROS scavenging in wheat. Conclusions Altogether, the obtained findings contribute to a better understanding of the functions of SnRK3.23 in wheat and offer genetic suggestions for improving drought resistance of wheat. |
| format | Article |
| id | doaj-art-c4e78ac8c0714dfcb44ee20a90e41fe3 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-c4e78ac8c0714dfcb44ee20a90e41fe32025-01-19T12:16:52ZengBMCBMC Plant Biology1471-22292025-01-0125111410.1186/s12870-025-06091-yTaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in ArabidopsisFeiyan Dong0Yide Liu1Huadong Zhang2Yaqian Li3Jinghan Song4Sheng Chen5Shuailei Wang6Zhanwang Zhu7Yan Li8Yike Liu9Institute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang UniversityInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementKey Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Coconstruction by Ministry and Province)/College of Agriculture, Yangtze UniversityInstitute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementAbstract Background Sucrose non-fermenting-1-related protein kinases (SnRKs) have been implicated in plant growth and stress responses. Although SnRK3.23 is known to be involved in drought stress, the underlying mechanism of resistance differs between Arabidopsis and rice, and little is known about its function in wheat. Results In the current work, TaSnRK3.23B was detected on the cell membrane and in the nucleus. TaSnRK3.23B overexpression in Arabidopsis promoted reactive oxygen species (ROS) scavenging via the accumulation of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and then conferred significant tolerance to drought. The prediction analysis, yeast two-hybrid, and bimolecular fluorescence complementation (BiFC) assays revealed that TaSnRK3.23B interacted with TaCBL2B and TaCBL6B, which are calcineurin B-like (CBL) proteins. The predicted model demonstrated that TaSnRK3 subfamily proteins participate in Ca2+ signaling mediated by TaCBL2B/TaCBL6B and subsequently provide drought stress tolerance by promoting ROS scavenging in wheat. Conclusions Altogether, the obtained findings contribute to a better understanding of the functions of SnRK3.23 in wheat and offer genetic suggestions for improving drought resistance of wheat.https://doi.org/10.1186/s12870-025-06091-yWheatTaSnRK3.23BEctopic expressionDrought stressCBL proteins |
| spellingShingle | Feiyan Dong Yide Liu Huadong Zhang Yaqian Li Jinghan Song Sheng Chen Shuailei Wang Zhanwang Zhu Yan Li Yike Liu TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis BMC Plant Biology Wheat TaSnRK3.23B Ectopic expression Drought stress CBL proteins |
| title | TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis |
| title_full | TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis |
| title_fullStr | TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis |
| title_full_unstemmed | TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis |
| title_short | TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis |
| title_sort | tasnrk3 23b a cbl interacting protein kinase of wheat confers drought stress tolerance by promoting ros scavenging in arabidopsis |
| topic | Wheat TaSnRK3.23B Ectopic expression Drought stress CBL proteins |
| url | https://doi.org/10.1186/s12870-025-06091-y |
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