The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation
Summary: Understanding the regulatory mechanisms underlying the plant heat stress response is important for developing climate-resilient crops, including rice (Oryza sativa). Here, we report that OsEIL5, one member of the ETHYLENE INSENSITIVE3-LIKE family, positively regulates rice heat tolerance at...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725000427 |
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| author | Jianping Liu Ke Wang Guining Wang Zhi Peng Tao Wang Yang Meng Jinli Huang Jiaohan Huo Xin Li Xiaoqi Zhu Feiyun Xu Qian Zhang Zhengrui Wang Hao Chen Ya Wang Weifeng Xu |
| author_facet | Jianping Liu Ke Wang Guining Wang Zhi Peng Tao Wang Yang Meng Jinli Huang Jiaohan Huo Xin Li Xiaoqi Zhu Feiyun Xu Qian Zhang Zhengrui Wang Hao Chen Ya Wang Weifeng Xu |
| author_sort | Jianping Liu |
| collection | DOAJ |
| description | Summary: Understanding the regulatory mechanisms underlying the plant heat stress response is important for developing climate-resilient crops, including rice (Oryza sativa). Here, we report that OsEIL5, one member of the ETHYLENE INSENSITIVE3-LIKE family, positively regulates rice heat tolerance at the seedling and reproductive stages. OsEIL5 directly binds to the promoter of OsPP91 (encoding one type 2C protein phosphatase) and activates its expression. OsPP91 is required for rice thermotolerance, and overexpressing OsPP91 in oseil5-1 partially rescues its heat sensitivity. The F box protein OsEBF1 interacts with OsEIL5 and degrades it through the ubiquitination pathway, resulting in the reduction of OsPP91 expression and ultimately weakening rice heat tolerance. Knocking out OsEIL5 in the EBF1R13 line partially reduces its extremely high heat tolerance. Taken together, our work uncovers a mechanism that finely regulates rice thermotolerance through the OsEBF1-OsEIL5-OsPP91 module at the posttranslational and transcriptional levels. |
| format | Article |
| id | doaj-art-c4d76168ca28477bb4ae92e6f4de4986 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-c4d76168ca28477bb4ae92e6f4de49862025-02-06T05:11:32ZengElsevierCell Reports2211-12472025-02-01442115271The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activationJianping Liu0Ke Wang1Guining Wang2Zhi Peng3Tao Wang4Yang Meng5Jinli Huang6Jiaohan Huo7Xin Li8Xiaoqi Zhu9Feiyun Xu10Qian Zhang11Zhengrui Wang12Hao Chen13Ya Wang14Weifeng Xu15Center for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350000, ChinaCenter for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCenter for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCenter for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCenter for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaCereal Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaCenter for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding authorSummary: Understanding the regulatory mechanisms underlying the plant heat stress response is important for developing climate-resilient crops, including rice (Oryza sativa). Here, we report that OsEIL5, one member of the ETHYLENE INSENSITIVE3-LIKE family, positively regulates rice heat tolerance at the seedling and reproductive stages. OsEIL5 directly binds to the promoter of OsPP91 (encoding one type 2C protein phosphatase) and activates its expression. OsPP91 is required for rice thermotolerance, and overexpressing OsPP91 in oseil5-1 partially rescues its heat sensitivity. The F box protein OsEBF1 interacts with OsEIL5 and degrades it through the ubiquitination pathway, resulting in the reduction of OsPP91 expression and ultimately weakening rice heat tolerance. Knocking out OsEIL5 in the EBF1R13 line partially reduces its extremely high heat tolerance. Taken together, our work uncovers a mechanism that finely regulates rice thermotolerance through the OsEBF1-OsEIL5-OsPP91 module at the posttranslational and transcriptional levels.http://www.sciencedirect.com/science/article/pii/S2211124725000427CP: Plants |
| spellingShingle | Jianping Liu Ke Wang Guining Wang Zhi Peng Tao Wang Yang Meng Jinli Huang Jiaohan Huo Xin Li Xiaoqi Zhu Feiyun Xu Qian Zhang Zhengrui Wang Hao Chen Ya Wang Weifeng Xu The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation Cell Reports CP: Plants |
| title | The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| title_full | The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| title_fullStr | The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| title_full_unstemmed | The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| title_short | The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| title_sort | osebf1 oseil5 ospp91 module regulates rice heat tolerance via ubiquitination and transcriptional activation |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725000427 |
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