Endothelin-1 Stimulates Monocytes in vitro to Release Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1
In the present study we examined whether endothelin-1 stimulation of human monocytes causes release of chemotactic factors. It was found that monocytes released neutrophil- and monocyte-chemotactic activity in a dose- and time-dependent manner in response to ET-1. ET-1 did not show any chemotactic a...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000207 |
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| _version_ | 1849766135154606080 |
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| author | E. Helset T. Sildnes Z. S. Konopski |
| author_facet | E. Helset T. Sildnes Z. S. Konopski |
| author_sort | E. Helset |
| collection | DOAJ |
| description | In the present study we examined whether endothelin-1 stimulation of
human monocytes causes release of chemotactic factors. It was found
that monocytes released neutrophil- and monocyte-chemotactic
activity in a dose- and time-dependent manner in response to ET-1.
ET-1 did not show any chemotactic activity by itself. NCA was
detected in monocyte supernatants in response to ET-1
(0.01–100 nM) after 1, 4, 8 and 24 h stimulation. MCA was
detected only after 24 h stimulation with ET-1 (0.1–100 nM).
Preincubation of the monocyte cultures with the lipoxygenase
inhibitors nordihydroguaiaretic acid (10−4 M) or
diethylcarbamazine (10−9 M) completely abolished
the appearance of NCA and MCA. NCA was neutralized by >
75% using a polyclonal antibody against human interleuktn-8.
The ET-1 induced release of IL-8 was confirmed by IL-8 ELISA. A
monoclonal antibody against human monocyte chemotactic protein-1
neutralized MCA by > 80%. It is concluded that ET-1
stimulation of monocytes in vitro causes release of
neutrophil- and monocyte-chemotactic activity identified as IL-8 and
MCP-I respectively. An intact lipoxygenase pathway is crucial for
this effect of ET-1 to occur. |
| format | Article |
| id | doaj-art-c4d753b39e6f45dfa49e15e75f8ca727 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c4d753b39e6f45dfa49e15e75f8ca7272025-08-20T03:04:40ZengWileyMediators of Inflammation0962-93511466-18611994-01-013215516010.1155/S0962935194000207Endothelin-1 Stimulates Monocytes in vitro to Release Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1E. Helset0T. Sildnes1Z. S. Konopski2Department of Anaesthesiology, Institute of Clinical Medicine, University of Tromsø, Tromsø N-9038, NorwayDepartment of Anaesthesiology, Institute of Clinical Medicine, University of Tromsø, Tromsø N-9038, NorwayDepartment of Experimental Pathology, Institute of Medical Biology, University of Tromsø, Tromsø N-9038, NorwayIn the present study we examined whether endothelin-1 stimulation of human monocytes causes release of chemotactic factors. It was found that monocytes released neutrophil- and monocyte-chemotactic activity in a dose- and time-dependent manner in response to ET-1. ET-1 did not show any chemotactic activity by itself. NCA was detected in monocyte supernatants in response to ET-1 (0.01–100 nM) after 1, 4, 8 and 24 h stimulation. MCA was detected only after 24 h stimulation with ET-1 (0.1–100 nM). Preincubation of the monocyte cultures with the lipoxygenase inhibitors nordihydroguaiaretic acid (10−4 M) or diethylcarbamazine (10−9 M) completely abolished the appearance of NCA and MCA. NCA was neutralized by > 75% using a polyclonal antibody against human interleuktn-8. The ET-1 induced release of IL-8 was confirmed by IL-8 ELISA. A monoclonal antibody against human monocyte chemotactic protein-1 neutralized MCA by > 80%. It is concluded that ET-1 stimulation of monocytes in vitro causes release of neutrophil- and monocyte-chemotactic activity identified as IL-8 and MCP-I respectively. An intact lipoxygenase pathway is crucial for this effect of ET-1 to occur.http://dx.doi.org/10.1155/S0962935194000207 |
| spellingShingle | E. Helset T. Sildnes Z. S. Konopski Endothelin-1 Stimulates Monocytes in vitro to Release Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 Mediators of Inflammation |
| title | Endothelin-1 Stimulates Monocytes in vitro to Release
Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 |
| title_full | Endothelin-1 Stimulates Monocytes in vitro to Release
Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 |
| title_fullStr | Endothelin-1 Stimulates Monocytes in vitro to Release
Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 |
| title_full_unstemmed | Endothelin-1 Stimulates Monocytes in vitro to Release
Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 |
| title_short | Endothelin-1 Stimulates Monocytes in vitro to Release
Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1 |
| title_sort | endothelin 1 stimulates monocytes in vitro to release chemotactic activity identified as interleukin 8 and monocyte chemotactic protein 1 |
| url | http://dx.doi.org/10.1155/S0962935194000207 |
| work_keys_str_mv | AT ehelset endothelin1stimulatesmonocytesinvitrotoreleasechemotacticactivityidentifiedasinterleukin8andmonocytechemotacticprotein1 AT tsildnes endothelin1stimulatesmonocytesinvitrotoreleasechemotacticactivityidentifiedasinterleukin8andmonocytechemotacticprotein1 AT zskonopski endothelin1stimulatesmonocytesinvitrotoreleasechemotacticactivityidentifiedasinterleukin8andmonocytechemotacticprotein1 |