Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction
Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in...
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Universitas Airlangga, Faculty of Veterinary Medicine
2024-10-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/56251 |
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| author | Reza Yesica Andreas Bandang Hardian Yudit Oktanella Shelly Kusumarini Gabriela Jeanne Giacynta Prasetyo |
| author_facet | Reza Yesica Andreas Bandang Hardian Yudit Oktanella Shelly Kusumarini Gabriela Jeanne Giacynta Prasetyo |
| author_sort | Reza Yesica |
| collection | DOAJ |
| description | Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61). |
| format | Article |
| id | doaj-art-c4cfaaac924e41de9f23012a4f4c8e02 |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-c4cfaaac924e41de9f23012a4f4c8e022025-02-02T23:06:15ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-10-017233534810.20473/jmv.vol7.iss2.2024.335-34854415Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain ReactionReza Yesica0https://orcid.org/0009-0009-2413-164XAndreas Bandang Hardian1https://orcid.org/0000-0002-2705-2412Yudit Oktanella2https://orcid.org/0000-0002-9573-882XShelly Kusumarini3https://orcid.org/0000-0002-7737-6044Gabriela Jeanne Giacynta Prasetyo4Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Brawijaya, IndonesiaLaboratory of Anatomic Pathology, Faculty of Veterinary Medicine, Universitas Brawijaya, IndonesiaLaboratory of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Brawijaya, IndonesiaLaboratory of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Brawijaya, IndonesiaUndergraduate Program, Faculty of Veterinary Medicine, Universitas Brawijaya, IndonesiaMurine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61).https://e-journal.unair.ac.id/JMV/article/view/56251its1polymerase chain reactionratstrypanosoma lewisi |
| spellingShingle | Reza Yesica Andreas Bandang Hardian Yudit Oktanella Shelly Kusumarini Gabriela Jeanne Giacynta Prasetyo Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction Jurnal Medik Veteriner its1 polymerase chain reaction rats trypanosoma lewisi |
| title | Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction |
| title_full | Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction |
| title_fullStr | Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction |
| title_full_unstemmed | Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction |
| title_short | Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction |
| title_sort | phylogenetic and genetic variation analysis of its1 gene of trypanosoma lewisi in wild rats using polymerase chain reaction |
| topic | its1 polymerase chain reaction rats trypanosoma lewisi |
| url | https://e-journal.unair.ac.id/JMV/article/view/56251 |
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