A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model
Objectives: This work aims at ameliorative evaluation of in vivo and in silico aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of Phyllanthus urinaria L. and Adhatoda vasica nees in High Fat Diet (HFD) induced Swiss albino mice. Methods: Male Swiss albino mice (23–25 g, n = 6)...
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Elsevier
2025-05-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031325000491 |
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| author | Pritimoni Das Manas Das Pranjan Barman Naba Kumar Hazarika Nabajyoti Goswami |
| author_facet | Pritimoni Das Manas Das Pranjan Barman Naba Kumar Hazarika Nabajyoti Goswami |
| author_sort | Pritimoni Das |
| collection | DOAJ |
| description | Objectives: This work aims at ameliorative evaluation of in vivo and in silico aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of Phyllanthus urinaria L. and Adhatoda vasica nees in High Fat Diet (HFD) induced Swiss albino mice. Methods: Male Swiss albino mice (23–25 g, n = 6) fed with 60 % HFD were treated with a low dose (550 mg/kg b.wt.) and high dose (920 mg/kg b.wt.) of the PHF for 12 weeks. In vivo experiments were performed in subcutaneous adipose tissue and white adipose tissue in HFD induced obese Swiss Albino mice through serum lipid profiling, qPCR analysis of adipogenic, lipolytic genes as well as in silico studies for detection of potent anti-obesity compound through LCMS, molecular docking and simulation based molecular dynamics analysis of target protein CD36. Results: The PHF-treated mice showed significant (p = 0.01) reduction of obesity by improving lipolysis and lowered adipogenesis than the non-treated obese group. The identified compound Granisetron showed higher binding energy as well as standard stability and notably higher interaction towards Granisetron in 100 ns with CD36 receptor than standard drug (∆Gbind value -106.32 ± 2.51). Conclusion: The study validated the efficacy of our novel PHF against HFD-induced obesity in a dose-related manner thereby establishing itself as a potential translational medicine, ensuring the utmost therapeutic value. |
| format | Article |
| id | doaj-art-c4adb742b4504bd8b05704e36fb6e2e2 |
| institution | DOAJ |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| series | Phytomedicine Plus |
| spelling | doaj-art-c4adb742b4504bd8b05704e36fb6e2e22025-08-20T03:09:34ZengElsevierPhytomedicine Plus2667-03132025-05-015210077610.1016/j.phyplu.2025.100776A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine modelPritimoni Das0Manas Das1Pranjan Barman2Naba Kumar Hazarika3Nabajyoti Goswami4Department of Zoology, Mangaldai College. Mangaldai 784125, India; Department of Zoology, Gauhati University, Guwahati 781014, IndiaCorresponding author.; Department of Zoology, Gauhati University, Guwahati 781014, IndiaDepartment of Biotechnology, Gauhati University, Guwahati 781014Department of Rasa Shasra and Bhaishajya Kalpana, Govt. Ayurvedic College & Hospital, Assam, Guwahati 781014Department of Bioengineering, Gauhati University, Guwahati 781014Objectives: This work aims at ameliorative evaluation of in vivo and in silico aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of Phyllanthus urinaria L. and Adhatoda vasica nees in High Fat Diet (HFD) induced Swiss albino mice. Methods: Male Swiss albino mice (23–25 g, n = 6) fed with 60 % HFD were treated with a low dose (550 mg/kg b.wt.) and high dose (920 mg/kg b.wt.) of the PHF for 12 weeks. In vivo experiments were performed in subcutaneous adipose tissue and white adipose tissue in HFD induced obese Swiss Albino mice through serum lipid profiling, qPCR analysis of adipogenic, lipolytic genes as well as in silico studies for detection of potent anti-obesity compound through LCMS, molecular docking and simulation based molecular dynamics analysis of target protein CD36. Results: The PHF-treated mice showed significant (p = 0.01) reduction of obesity by improving lipolysis and lowered adipogenesis than the non-treated obese group. The identified compound Granisetron showed higher binding energy as well as standard stability and notably higher interaction towards Granisetron in 100 ns with CD36 receptor than standard drug (∆Gbind value -106.32 ± 2.51). Conclusion: The study validated the efficacy of our novel PHF against HFD-induced obesity in a dose-related manner thereby establishing itself as a potential translational medicine, ensuring the utmost therapeutic value.http://www.sciencedirect.com/science/article/pii/S2667031325000491ObesityPolyherbal formulationAdipogenesisLipolysisMolecular dynamics simulation |
| spellingShingle | Pritimoni Das Manas Das Pranjan Barman Naba Kumar Hazarika Nabajyoti Goswami A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model Phytomedicine Plus Obesity Polyherbal formulation Adipogenesis Lipolysis Molecular dynamics simulation |
| title | A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model |
| title_full | A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model |
| title_fullStr | A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model |
| title_full_unstemmed | A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model |
| title_short | A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model |
| title_sort | synergistic anti obesity effects of phyllanthus urinaria and adhatoda vasica nees formulation an investigation in swiss albino murine model |
| topic | Obesity Polyherbal formulation Adipogenesis Lipolysis Molecular dynamics simulation |
| url | http://www.sciencedirect.com/science/article/pii/S2667031325000491 |
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