Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice
The therapeutic effect of gamma-aminobutyric acid (GABA) on diabetes was spread as one of the alarming epidemics worldwide. The study aims to investigate the function of Lactobacillus brevis KLDS1.0727 and KLDS1.0373 strains as glutamic acid decarboxylase 65 (GAD65) carriers capable of generating GA...
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000835 |
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| author | Amro Abdelazez Heba Abdelmotaal Smith Etareri Evivie Maha Bikheet Rokayya Sami Hassan Mohamed Xiangchen Meng |
| author_facet | Amro Abdelazez Heba Abdelmotaal Smith Etareri Evivie Maha Bikheet Rokayya Sami Hassan Mohamed Xiangchen Meng |
| author_sort | Amro Abdelazez |
| collection | DOAJ |
| description | The therapeutic effect of gamma-aminobutyric acid (GABA) on diabetes was spread as one of the alarming epidemics worldwide. The study aims to investigate the function of Lactobacillus brevis KLDS1.0727 and KLDS1.0373 strains as glutamic acid decarboxylase 65 (GAD65) carriers capable of generating GABA by comparing in vitro free and freeze-dried models and GABA intervention in vivo. PCR amplification of gad and in vitro i.e., (growth rate, viability at different pH, bile tolerance, and survivability in simulated gastric juice) were performed. In vivo experiments were conducted in 7 groups of C57BL/6J mice. Each group was injected with streptozotocin (ContSTZ, INSSTZ, LAC1STZ, LAC1MFDSTZ, LAC2STZ, LAC2MFDSTZ) daily except for the control (Cont). One group was injected with insulin (INSSTZ). The body weight and hyperglycemia in the blood were assessed weekly, post-euthanasia blood plasma parameters, insulin, and histological examination were evaluated. Results indicated L. brevis strains demonstrated a great tolerance to bile and simulated gastric juice in vitro (P < 0.05). ContSTZ had the highest average glucose level (6.84 ± 6.46) mmol/L while INSSTZ expressed dramatically decreed in glucose level and displayed a significant decline in the average of weekly blood glucose (−5.74 ± 3.08) mmol/L. The lowest body weight (ContSTZ) was (19.30 ± 0.25) g. Based on the blood plasma analysis, L. brevis strains improved good cholesterol properties, liver and kidney functions, where most of these parameters fall within the average the reference range and prevent the development of symptoms of type 1 diabetes in vivo. As recommended, L. brevis should be commonly distributed as a postbiotic GABA in pharmaceutical and nutritional applications. |
| format | Article |
| id | doaj-art-c4661d3f9c424a17a08a5c6a34b61f0f |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c4661d3f9c424a17a08a5c6a34b61f0f2025-02-03T06:51:02ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-01111165176Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic miceAmro Abdelazez0Heba Abdelmotaal1Smith Etareri Evivie2Maha Bikheet3Rokayya Sami4Hassan Mohamed5Xiangchen Meng6Key Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin 150030, China; College of Agriculture and Forestry, Linyi University, Linyi 276005, China; Department of Dairy Microbiology, Animal Production Research Institute, Agriculture Research Centre, Dokki, Giza 12618, Egypt; Corresponding authors at: Key Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin 150030, China.College of Agriculture and Forestry, Linyi University, Linyi 276005, China; Department of Microbiology, Soil, Water, Environment, and Microbiology Research Institute, Agriculture Research Centre, Giza 12619, EgyptKey Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin 150030, China; Department of Food Science & Hunan Nutrition, Faculty of Agriculture, University of Benin, Benin City 300001, NigeriaDepartment of Dairy Science, Faculty of Agriculture, Minia University, Minia 61511, EgyptDepartment of Food Science and Nutrition, College of Sciences, Taif University, P. O. 11099, Taif 21944, Saudi ArabiaColin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo 255049, China; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut 71524, EgyptKey Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin 150030, China; Corresponding authors at: Key Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin 150030, China.The therapeutic effect of gamma-aminobutyric acid (GABA) on diabetes was spread as one of the alarming epidemics worldwide. The study aims to investigate the function of Lactobacillus brevis KLDS1.0727 and KLDS1.0373 strains as glutamic acid decarboxylase 65 (GAD65) carriers capable of generating GABA by comparing in vitro free and freeze-dried models and GABA intervention in vivo. PCR amplification of gad and in vitro i.e., (growth rate, viability at different pH, bile tolerance, and survivability in simulated gastric juice) were performed. In vivo experiments were conducted in 7 groups of C57BL/6J mice. Each group was injected with streptozotocin (ContSTZ, INSSTZ, LAC1STZ, LAC1MFDSTZ, LAC2STZ, LAC2MFDSTZ) daily except for the control (Cont). One group was injected with insulin (INSSTZ). The body weight and hyperglycemia in the blood were assessed weekly, post-euthanasia blood plasma parameters, insulin, and histological examination were evaluated. Results indicated L. brevis strains demonstrated a great tolerance to bile and simulated gastric juice in vitro (P < 0.05). ContSTZ had the highest average glucose level (6.84 ± 6.46) mmol/L while INSSTZ expressed dramatically decreed in glucose level and displayed a significant decline in the average of weekly blood glucose (−5.74 ± 3.08) mmol/L. The lowest body weight (ContSTZ) was (19.30 ± 0.25) g. Based on the blood plasma analysis, L. brevis strains improved good cholesterol properties, liver and kidney functions, where most of these parameters fall within the average the reference range and prevent the development of symptoms of type 1 diabetes in vivo. As recommended, L. brevis should be commonly distributed as a postbiotic GABA in pharmaceutical and nutritional applications.http://www.sciencedirect.com/science/article/pii/S2213453021000835DiabetesGamma-aminobutyric acidPostbioticsLactobacillus brevisIn vitroC57BL/6J mice |
| spellingShingle | Amro Abdelazez Heba Abdelmotaal Smith Etareri Evivie Maha Bikheet Rokayya Sami Hassan Mohamed Xiangchen Meng Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice Food Science and Human Wellness Diabetes Gamma-aminobutyric acid Postbiotics Lactobacillus brevis In vitro C57BL/6J mice |
| title | Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice |
| title_full | Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice |
| title_fullStr | Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice |
| title_full_unstemmed | Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice |
| title_short | Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice |
| title_sort | verification of lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma aminobutyric acid in streptozotocin induced diabetic mice |
| topic | Diabetes Gamma-aminobutyric acid Postbiotics Lactobacillus brevis In vitro C57BL/6J mice |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000835 |
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