Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses
Plant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA)...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/7214087 |
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| author | Deepak M. Kasote Ritesh Ghosh Jun Young Chung Jonggeun Kim Inhwan Bae Hanhong Bae |
| author_facet | Deepak M. Kasote Ritesh Ghosh Jun Young Chung Jonggeun Kim Inhwan Bae Hanhong Bae |
| author_sort | Deepak M. Kasote |
| collection | DOAJ |
| description | Plant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) fluctuate upon abiotic stress exposure. At present, there is not any single suitable liquid chromatography-mass spectrometry (LC-MS) method for simultaneous analysis of BR and other plant hormones involved in abiotic stresses. In the present study, we developed a simple, sensitive, and rapid method for simultaneous analysis of five major plant hormones, ZA, ABA, JA, SA, and BR, which are directly or indirectly involved in drought and salt stresses. The optimized extraction procedure was simple and easy to use for simultaneous measurement of these plant hormones in Arabidopsis thaliana. The developed method is highly reproducible and can be adapted for simultaneous measurement of changes in plant hormones (ZA, ABA, JA, SA, and BR) in response to abiotic stresses in plants like A. thaliana and tomato. |
| format | Article |
| id | doaj-art-c44b0d033cf74bcf982685bbdde30dba |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-c44b0d033cf74bcf982685bbdde30dba2025-08-20T02:18:47ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/72140877214087Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic StressesDeepak M. Kasote0Ritesh Ghosh1Jun Young Chung2Jonggeun Kim3Inhwan Bae4Hanhong Bae5School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of KoreaDepartment of Orthopedic Surgery, School of Medicine, Ajou University, Suwon 442-749, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of KoreaCollege of Pharmacy, Chungang University, Seoul 156-756, Republic of KoreaSchool of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of KoreaPlant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) fluctuate upon abiotic stress exposure. At present, there is not any single suitable liquid chromatography-mass spectrometry (LC-MS) method for simultaneous analysis of BR and other plant hormones involved in abiotic stresses. In the present study, we developed a simple, sensitive, and rapid method for simultaneous analysis of five major plant hormones, ZA, ABA, JA, SA, and BR, which are directly or indirectly involved in drought and salt stresses. The optimized extraction procedure was simple and easy to use for simultaneous measurement of these plant hormones in Arabidopsis thaliana. The developed method is highly reproducible and can be adapted for simultaneous measurement of changes in plant hormones (ZA, ABA, JA, SA, and BR) in response to abiotic stresses in plants like A. thaliana and tomato.http://dx.doi.org/10.1155/2016/7214087 |
| spellingShingle | Deepak M. Kasote Ritesh Ghosh Jun Young Chung Jonggeun Kim Inhwan Bae Hanhong Bae Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses International Journal of Analytical Chemistry |
| title | Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses |
| title_full | Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses |
| title_fullStr | Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses |
| title_full_unstemmed | Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses |
| title_short | Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses |
| title_sort | multiple reaction monitoring mode based liquid chromatography mass spectrometry method for simultaneous quantification of brassinolide and other plant hormones involved in abiotic stresses |
| url | http://dx.doi.org/10.1155/2016/7214087 |
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