Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers

Six hybrids were subjected to chromatographic analyses by HPLC for the determination of phytochemicals such as capsaicinoid, polyphenol, and vitamin C. The dynamics of ripening of 4 of the hybrids were also characterised. Seven capsaicinoids could be separated and determined; the major compounds wer...

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Main Authors: Zsuzsa Nagy, Hussein Daood, Zsuzsanna Ambrózy, Lajos Helyes
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2015/102125
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author Zsuzsa Nagy
Hussein Daood
Zsuzsanna Ambrózy
Lajos Helyes
author_facet Zsuzsa Nagy
Hussein Daood
Zsuzsanna Ambrózy
Lajos Helyes
author_sort Zsuzsa Nagy
collection DOAJ
description Six hybrids were subjected to chromatographic analyses by HPLC for the determination of phytochemicals such as capsaicinoid, polyphenol, and vitamin C. The dynamics of ripening of 4 of the hybrids were also characterised. Seven capsaicinoids could be separated and determined; the major compounds were nordihydrocapsaicin, capsaicin, and dihydrocapsaicin, while homocapsaicin and homodihydrocapsaicin derivatives were detected as minor constituents. Capsaicin content ranged between 95.5 ± 4.15 and 1610.2 ± 91.46 μg/g FW, and the highest value was found in Bandai (C. frutescens) at the green ripening stage. The major capsaicinoids had a decreasing tendency in Bandai and Chili 3735 hybrids, while no change was observed in Beibeihong and Lolo during ripening. Nine polyphenol compounds were detected including 8 flavonoids and a nonflavonoid compound in the pods of all hybrids. The major components were naringenin-diglucoside, catechin, and vanillic acid-derivative and luteolin-glucoside. Naringenin-diglucoside ranged from 93.5 ± 4.26 to 368.8 ± 30.77 μg/g FW. Except vanillic acid-derivative, dominant polyphenols increased or remained unchanged during ripening. As for vitamin C, its content tended to increase with the advance in ripening in all hybrids included in this study. The highest value of 3689.4 ± 39.50 μg/g FW was recorded in Fire Flame hybrid.
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spelling doaj-art-c42acebae49e4b39b37166a404eeb19b2025-02-03T05:58:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/102125102125Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili PeppersZsuzsa Nagy0Hussein Daood1Zsuzsanna Ambrózy2Lajos Helyes3Institute of Horticulture, Faculty of Agriculture and Environmental Sciences, Szent István University, Páter Károly Street 1, Gödöllő 2100, HungaryRegional Knowledge Centre, Szent István University, Páter Károly Street 1, Gödöllő 2100, HungaryInstitute of Horticulture, Faculty of Agriculture and Environmental Sciences, Szent István University, Páter Károly Street 1, Gödöllő 2100, HungaryInstitute of Horticulture, Faculty of Agriculture and Environmental Sciences, Szent István University, Páter Károly Street 1, Gödöllő 2100, HungarySix hybrids were subjected to chromatographic analyses by HPLC for the determination of phytochemicals such as capsaicinoid, polyphenol, and vitamin C. The dynamics of ripening of 4 of the hybrids were also characterised. Seven capsaicinoids could be separated and determined; the major compounds were nordihydrocapsaicin, capsaicin, and dihydrocapsaicin, while homocapsaicin and homodihydrocapsaicin derivatives were detected as minor constituents. Capsaicin content ranged between 95.5 ± 4.15 and 1610.2 ± 91.46 μg/g FW, and the highest value was found in Bandai (C. frutescens) at the green ripening stage. The major capsaicinoids had a decreasing tendency in Bandai and Chili 3735 hybrids, while no change was observed in Beibeihong and Lolo during ripening. Nine polyphenol compounds were detected including 8 flavonoids and a nonflavonoid compound in the pods of all hybrids. The major components were naringenin-diglucoside, catechin, and vanillic acid-derivative and luteolin-glucoside. Naringenin-diglucoside ranged from 93.5 ± 4.26 to 368.8 ± 30.77 μg/g FW. Except vanillic acid-derivative, dominant polyphenols increased or remained unchanged during ripening. As for vitamin C, its content tended to increase with the advance in ripening in all hybrids included in this study. The highest value of 3689.4 ± 39.50 μg/g FW was recorded in Fire Flame hybrid.http://dx.doi.org/10.1155/2015/102125
spellingShingle Zsuzsa Nagy
Hussein Daood
Zsuzsanna Ambrózy
Lajos Helyes
Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
Journal of Analytical Methods in Chemistry
title Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
title_full Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
title_fullStr Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
title_full_unstemmed Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
title_short Determination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
title_sort determination of polyphenols capsaicinoids and vitamin c in new hybrids of chili peppers
url http://dx.doi.org/10.1155/2015/102125
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AT zsuzsannaambrozy determinationofpolyphenolscapsaicinoidsandvitamincinnewhybridsofchilipeppers
AT lajoshelyes determinationofpolyphenolscapsaicinoidsandvitamincinnewhybridsofchilipeppers