The Construction of Heterothallic Strains of <i>Komagataella kurtzmanii</i> Using the I-SceI Meganuclease

The Construction of Heterothallic Strains of <i>Komagataella kurtzmanii</i> Using the I-SceI Meganuclease

The methylotrophic yeast <i>Komagataella kurtzmanii</i> belongs to the group of homothallic fungi that are able to spontaneously change their mating type by inversion of chromosomal DNA in the MAT locus region. As a result, natural and genetically engineered cultures of these yeasts typi...

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Bibliographic Details
Main Authors: Daria D. Sokolova, Philipp I. Akentyev, Kristina O. Petrova, Lyudmila V. Lyutova, Aleksei A. Korzhenkov, Irek I. Gubaidullin, Stepan V. Toshchakov, Dmitry G. Kozlov
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biomolecules
Subjects:
<i>Komagataella kurtzmanii</i>
<i>Komagataella phaffii</i>
heterothallic yeast
cell mating type
I-SceI meganuclease
repair
Online Access:https://www.mdpi.com/2218-273X/15/1/97
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