Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
Cinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm g...
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2012-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1100/2012/549265 |
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author | Vineeta Singh Atul Kumar Gupta S. P. Singh Anil Kumar |
author_facet | Vineeta Singh Atul Kumar Gupta S. P. Singh Anil Kumar |
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description | Cinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm genotypes, a newly emerging mass spectral ionization technique direct time in real time (DART) is very helpful. The DART ion source has been used to analyze an extremely wide range of phytochemicals present in leaves of Cinnamomum tamala. Ten genotypes were assessed for the presence of different phytochemicals. Phytochemical analysis showed the presence of mainly terpenes and phenols. These constituents vary in the different genotypes of Cinnamomum tamala. Principal component analysis has also been employed to analyze the DART data of these Cinnamomum genotypes. The result shows that the genotype of Cinnamomum tamala could be differentiated using DART MS data. The active components present in Cinnamomum tamala may be contributing significantly to high amount of antioxidant property of leaves and, in turn, conditional effects for diabetic patients. |
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language | English |
publishDate | 2012-01-01 |
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spelling | doaj-art-c3e75180b18543308062f90d4070cd702025-02-03T01:22:48ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/549265549265Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm GenotypesVineeta Singh0Atul Kumar Gupta1S. P. Singh2Anil Kumar3Department of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Pharmacology & Toxicology, College of Veterinary Science, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaCinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm genotypes, a newly emerging mass spectral ionization technique direct time in real time (DART) is very helpful. The DART ion source has been used to analyze an extremely wide range of phytochemicals present in leaves of Cinnamomum tamala. Ten genotypes were assessed for the presence of different phytochemicals. Phytochemical analysis showed the presence of mainly terpenes and phenols. These constituents vary in the different genotypes of Cinnamomum tamala. Principal component analysis has also been employed to analyze the DART data of these Cinnamomum genotypes. The result shows that the genotype of Cinnamomum tamala could be differentiated using DART MS data. The active components present in Cinnamomum tamala may be contributing significantly to high amount of antioxidant property of leaves and, in turn, conditional effects for diabetic patients.http://dx.doi.org/10.1100/2012/549265 |
spellingShingle | Vineeta Singh Atul Kumar Gupta S. P. Singh Anil Kumar Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes The Scientific World Journal |
title | Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes |
title_full | Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes |
title_fullStr | Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes |
title_full_unstemmed | Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes |
title_short | Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes |
title_sort | direct analysis in real time by mass spectrometric technique for determining the variation in metabolite profiles of cinnamomum tamala nees and eberm genotypes |
url | http://dx.doi.org/10.1100/2012/549265 |
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