Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens
Polyphenols are among the natural antioxidants that have been exploited in recent years for their safe and effective ability against oxidative stress. This project aimed to optimize 3 factor-conditions affecting total polyphenols extracted from Perilla frutescens (L.) Britt as well as DNA protectio...
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HUJOS
2024-06-01
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| Series: | Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên |
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| Online Access: | https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7087 |
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| author | Ngoc Thien Kim Nguyen Huynh Anh Vu Truong Hoang Khue Tu Nguyen |
| author_facet | Ngoc Thien Kim Nguyen Huynh Anh Vu Truong Hoang Khue Tu Nguyen |
| author_sort | Ngoc Thien Kim Nguyen |
| collection | DOAJ |
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Polyphenols are among the natural antioxidants that have been exploited in recent years for their safe and effective ability against oxidative stress. This project aimed to optimize 3 factor-conditions affecting total polyphenols extracted from Perilla frutescens (L.) Britt as well as DNA protection activity of polyphenols for applications in the fields of dietary supplements, pharmaceuticals, and health care. All extracts contained phenolic compounds and exhibited good antioxidant ability through a ferric reducing antioxidant power assay. The total polyphenolic compounds varied from 6.056 ± 0.08 to 9.630 ± 0.127 mg of gallic acid equivalents (GAE) per 1 g dry weight (dw). The highest phenolic content yield was extracted at 70°C for 60 minutes at a pH of 7.0. However, a sample with the highest polyphenol content had a lower residual DNA concentration than the extract (55°C, 60 min, and pH 6.0) with the greatest reducing power. The result of the DNA protection assay also indicated that the extraction concentration and pH condition had a significant effect on preventing DNA from being damaged by free radicals. The study found the conditions for improving polyphenol in the extraction of P. frutescens (L.) Britt with the aid of Box-Behnken Design. This research also proposed that P. frutescens (L.) Britt is a good source showing DNA protection and antioxidant activity for healthcare.
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| format | Article |
| id | doaj-art-c3e46585ebea4072b943ffd7644113a5 |
| institution | Kabale University |
| issn | 1859-1388 2615-9678 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | HUJOS |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên |
| spelling | doaj-art-c3e46585ebea4072b943ffd7644113a52025-02-03T01:57:38ZengHUJOSTạp chí Khoa học Đại học Huế: Khoa học Tự nhiên1859-13882615-96782024-06-011331B10.26459/hueunijns.v133i1B.7087Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescensNgoc Thien Kim Nguyen0Huynh Anh Vu Truong1Hoang Khue Tu Nguyen2School of Biotechnology, HCMC International University, VietnamMicrobiology laboratory, Center of Analytical Services and Experimentation HCMC (CASE), VietnamSchool of Biotechnology, HCMC International University, Vietnam Polyphenols are among the natural antioxidants that have been exploited in recent years for their safe and effective ability against oxidative stress. This project aimed to optimize 3 factor-conditions affecting total polyphenols extracted from Perilla frutescens (L.) Britt as well as DNA protection activity of polyphenols for applications in the fields of dietary supplements, pharmaceuticals, and health care. All extracts contained phenolic compounds and exhibited good antioxidant ability through a ferric reducing antioxidant power assay. The total polyphenolic compounds varied from 6.056 ± 0.08 to 9.630 ± 0.127 mg of gallic acid equivalents (GAE) per 1 g dry weight (dw). The highest phenolic content yield was extracted at 70°C for 60 minutes at a pH of 7.0. However, a sample with the highest polyphenol content had a lower residual DNA concentration than the extract (55°C, 60 min, and pH 6.0) with the greatest reducing power. The result of the DNA protection assay also indicated that the extraction concentration and pH condition had a significant effect on preventing DNA from being damaged by free radicals. The study found the conditions for improving polyphenol in the extraction of P. frutescens (L.) Britt with the aid of Box-Behnken Design. This research also proposed that P. frutescens (L.) Britt is a good source showing DNA protection and antioxidant activity for healthcare. https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7087Perilla frutescens (L.) BrittOptimizationBox-Behnken design (BBD)total polyphenol content (TPC)ferric reducing antioxidant power (FRAP)DNA protection |
| spellingShingle | Ngoc Thien Kim Nguyen Huynh Anh Vu Truong Hoang Khue Tu Nguyen Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên Perilla frutescens (L.) Britt Optimization Box-Behnken design (BBD) total polyphenol content (TPC) ferric reducing antioxidant power (FRAP) DNA protection |
| title | Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens |
| title_full | Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens |
| title_fullStr | Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens |
| title_full_unstemmed | Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens |
| title_short | Optimizing conditions to improve polyphenol content and screening antioxidant capacity with DNA protection activity of Perilla frutescens |
| title_sort | optimizing conditions to improve polyphenol content and screening antioxidant capacity with dna protection activity of perilla frutescens |
| topic | Perilla frutescens (L.) Britt Optimization Box-Behnken design (BBD) total polyphenol content (TPC) ferric reducing antioxidant power (FRAP) DNA protection |
| url | https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7087 |
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