Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran
ABSTRACT Background Liver flukes from the genus Fasciola are the causative agents for human and livestock fascioliasis. Accurate identification of Fasciola spp. is essential to understanding the epidemiology of fascioliasis. This study aimed to determine the morphometric and molecular characterizati...
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| Format: | Article |
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70097 |
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| author | Hossein Galavani Saber Raeghi Mehdi Karamian Negin Torabi Ali Haniloo |
| author_facet | Hossein Galavani Saber Raeghi Mehdi Karamian Negin Torabi Ali Haniloo |
| author_sort | Hossein Galavani |
| collection | DOAJ |
| description | ABSTRACT Background Liver flukes from the genus Fasciola are the causative agents for human and livestock fascioliasis. Accurate identification of Fasciola spp. is essential to understanding the epidemiology of fascioliasis. This study aimed to determine the morphometric and molecular characterization of Fasciola spp. in livestock from Northwestern provinces of Iran. Methods Five hundred adult Fasciola flukes were obtained from different definitive hosts (cattle, sheep, goats, and buffaloes) in four local abattoirs in the northwestern provinces of Iran (West‐Azerbaijan, East‐Azerbaijan, Ardabil, and Zanjan) from September 2021 to August 2022. All samples were examined by morphometric criteria; then, 49 samples were identified using PCR‐RFLP based on ITS1 region and 23 sequence of isolates analyzed by cox1 marker. PCR‐RFLP methods compared morphometric results, and cox1 gene sequences were used to confirm PCR‐RFLP results and phylogenetic analysis. Results The differences between the body length, body width, cephalic cone length, cephalic cone width, body area, and distance between the ventral sucker and posterior end of the body in two species were significant (p < 0.05). Based on the morphometric criteria, 139 samples (27.8%) were identified as Fasciola gigantica and 361 (72.2%) as Fasciola hepatica. Similarly, PCR‐RFLP analysis of ITS1 region confirmed morphometric results. No hybrid forms of Fasciola were detected. Partial sequences of cox1 showed 13 variable sites with eight haplotypes in F. hepatica and 12 variable sites with five haplotypes in F. gigantica. Conclusion Based on the results of this study, the PCR‐RFLP method can be used to confirm the morphological method of Fasciola species, but it is insufficient to study their genetic diversity. Also, sequences of cox1 results of the present study showed that F. hepatica and F. gigantica species in the Northern provinces of Iran have different genetic structures and haplotypes. |
| format | Article |
| id | doaj-art-c3e17c84855840b4a038a40902a415ed |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-c3e17c84855840b4a038a40902a415ed2025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70097Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of IranHossein Galavani0Saber Raeghi1Mehdi Karamian2Negin Torabi3Ali Haniloo4Department of Medical Parasitology and Mycology School of Medicine Zanjan University of Medical Sciences Zanjan IranDepartment of Medical Parasitology and Mycology Urmia University of Medical Sciences Urmia IranDepartment of Medical Parasitology and Mycology School of Medicine Zanjan University of Medical Sciences Zanjan IranDepartment of Medical Parasitology and Mycology School of Medicine Zanjan University of Medical Sciences Zanjan IranDepartment of Medical Parasitology and Mycology School of Medicine Zanjan University of Medical Sciences Zanjan IranABSTRACT Background Liver flukes from the genus Fasciola are the causative agents for human and livestock fascioliasis. Accurate identification of Fasciola spp. is essential to understanding the epidemiology of fascioliasis. This study aimed to determine the morphometric and molecular characterization of Fasciola spp. in livestock from Northwestern provinces of Iran. Methods Five hundred adult Fasciola flukes were obtained from different definitive hosts (cattle, sheep, goats, and buffaloes) in four local abattoirs in the northwestern provinces of Iran (West‐Azerbaijan, East‐Azerbaijan, Ardabil, and Zanjan) from September 2021 to August 2022. All samples were examined by morphometric criteria; then, 49 samples were identified using PCR‐RFLP based on ITS1 region and 23 sequence of isolates analyzed by cox1 marker. PCR‐RFLP methods compared morphometric results, and cox1 gene sequences were used to confirm PCR‐RFLP results and phylogenetic analysis. Results The differences between the body length, body width, cephalic cone length, cephalic cone width, body area, and distance between the ventral sucker and posterior end of the body in two species were significant (p < 0.05). Based on the morphometric criteria, 139 samples (27.8%) were identified as Fasciola gigantica and 361 (72.2%) as Fasciola hepatica. Similarly, PCR‐RFLP analysis of ITS1 region confirmed morphometric results. No hybrid forms of Fasciola were detected. Partial sequences of cox1 showed 13 variable sites with eight haplotypes in F. hepatica and 12 variable sites with five haplotypes in F. gigantica. Conclusion Based on the results of this study, the PCR‐RFLP method can be used to confirm the morphological method of Fasciola species, but it is insufficient to study their genetic diversity. Also, sequences of cox1 results of the present study showed that F. hepatica and F. gigantica species in the Northern provinces of Iran have different genetic structures and haplotypes.https://doi.org/10.1002/vms3.70097cox1Fasciola sppIranITS1PCR‐RFLPphylogenetic |
| spellingShingle | Hossein Galavani Saber Raeghi Mehdi Karamian Negin Torabi Ali Haniloo Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran Veterinary Medicine and Science cox1 Fasciola spp Iran ITS1 PCR‐RFLP phylogenetic |
| title | Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran |
| title_full | Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran |
| title_fullStr | Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran |
| title_full_unstemmed | Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran |
| title_short | Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran |
| title_sort | morphometric and molecular characterization of fasciola spp in livestock from northwestern provinces of iran |
| topic | cox1 Fasciola spp Iran ITS1 PCR‐RFLP phylogenetic |
| url | https://doi.org/10.1002/vms3.70097 |
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